Predictive value and regulatory mechanism of serum miR-499a-5p on myocardial dysfunction in sepsis
Abstract Background This study sought to investigate the predictive value and regulatory mechanism of serum miR-499a-5p in sepsis-induced myocardial dysfunction (SIMD). Methods A total of 60 patients with sepsis and 60 healthy volunteers were enrolled in this study. The serum levels of miRNAs (miR-4...
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | Journal of Cardiothoracic Surgery |
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| Online Access: | https://doi.org/10.1186/s13019-021-01679-5 |
| _version_ | 1819109182535630848 |
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| author | Chuang Yang Kun Wen |
| author_facet | Chuang Yang Kun Wen |
| author_sort | Chuang Yang |
| collection | DOAJ |
| description | Abstract Background This study sought to investigate the predictive value and regulatory mechanism of serum miR-499a-5p in sepsis-induced myocardial dysfunction (SIMD). Methods A total of 60 patients with sepsis and 60 healthy volunteers were enrolled in this study. The serum levels of miRNAs (miR-451, miR-378 and miR-499a-5p) were detected. Receiver operating characteristic curve and logistic regression analysis were used to evaluate the diagnostic and prognostic value of miR-499a-5p in SIMD patients. AC16 cells were used to establish SIMD model in vitro using lipopolysaccharide (LPS). An analysis was conducted for miR-499a-5p expression, cell viability, and the concentration of creatine kinase-MB isoform (CK-MB), brain natriuretic peptide (BNP), superoxide dismutase (SOD) and cytochrome C oxidase IV (COX IV). The downstream target of miR-499a-5p was verified. Results Our results revealed a poor expression of miR-499a-5p in the serum of SIMD patients, while no significant difference was evident for miR-451 and miR-378. The level of miR-499a-5p in the survival group was higher than the non-survival group. miR-499a-5p elicited good diagnostic and prognostic value for SIMD. Our findings revealed that miR-499a-5p was decreased significantly in LPS-treated cardiomyocytes. After overexpression of miR-499a-5p, the cell viability increased, and the concentrations of CK-MB and BNP were decreased, while the concentrations of SOD and COX IV were increased. EIF4E was validated as the target of miR-499a-5p. After overexpression of EIF4E, the cell viability was decreased and the concentrations of CK-MB and BNP were increased while the concentrations of SOD and COX IV were decreased. Conclusion The level of miR-499a-5p is weak in SIMD patients. miR-499a-5p has a good diagnostic and prognostic value for SIMD by inhibiting EIF4E transcription. |
| first_indexed | 2024-12-22T03:21:46Z |
| format | Article |
| id | doaj.art-15a34a0fb93349539f0dd25f11f1fab0 |
| institution | Directory Open Access Journal |
| issn | 1749-8090 |
| language | English |
| last_indexed | 2024-12-22T03:21:46Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Cardiothoracic Surgery |
| spelling | doaj.art-15a34a0fb93349539f0dd25f11f1fab02022-12-21T18:40:42ZengBMCJournal of Cardiothoracic Surgery1749-80902021-10-0116111010.1186/s13019-021-01679-5Predictive value and regulatory mechanism of serum miR-499a-5p on myocardial dysfunction in sepsisChuang Yang0Kun Wen1Department of Critical Care Medicine, The Second Hospital of Shandong UniversityDepartment of Critical Care Medicine, The Second Hospital of Shandong UniversityAbstract Background This study sought to investigate the predictive value and regulatory mechanism of serum miR-499a-5p in sepsis-induced myocardial dysfunction (SIMD). Methods A total of 60 patients with sepsis and 60 healthy volunteers were enrolled in this study. The serum levels of miRNAs (miR-451, miR-378 and miR-499a-5p) were detected. Receiver operating characteristic curve and logistic regression analysis were used to evaluate the diagnostic and prognostic value of miR-499a-5p in SIMD patients. AC16 cells were used to establish SIMD model in vitro using lipopolysaccharide (LPS). An analysis was conducted for miR-499a-5p expression, cell viability, and the concentration of creatine kinase-MB isoform (CK-MB), brain natriuretic peptide (BNP), superoxide dismutase (SOD) and cytochrome C oxidase IV (COX IV). The downstream target of miR-499a-5p was verified. Results Our results revealed a poor expression of miR-499a-5p in the serum of SIMD patients, while no significant difference was evident for miR-451 and miR-378. The level of miR-499a-5p in the survival group was higher than the non-survival group. miR-499a-5p elicited good diagnostic and prognostic value for SIMD. Our findings revealed that miR-499a-5p was decreased significantly in LPS-treated cardiomyocytes. After overexpression of miR-499a-5p, the cell viability increased, and the concentrations of CK-MB and BNP were decreased, while the concentrations of SOD and COX IV were increased. EIF4E was validated as the target of miR-499a-5p. After overexpression of EIF4E, the cell viability was decreased and the concentrations of CK-MB and BNP were increased while the concentrations of SOD and COX IV were decreased. Conclusion The level of miR-499a-5p is weak in SIMD patients. miR-499a-5p has a good diagnostic and prognostic value for SIMD by inhibiting EIF4E transcription.https://doi.org/10.1186/s13019-021-01679-5SepsisMyocardial dysfunctionmiR-499a-5pEIF4ECK-MBBNP |
| spellingShingle | Chuang Yang Kun Wen Predictive value and regulatory mechanism of serum miR-499a-5p on myocardial dysfunction in sepsis Journal of Cardiothoracic Surgery Sepsis Myocardial dysfunction miR-499a-5p EIF4E CK-MB BNP |
| title | Predictive value and regulatory mechanism of serum miR-499a-5p on myocardial dysfunction in sepsis |
| title_full | Predictive value and regulatory mechanism of serum miR-499a-5p on myocardial dysfunction in sepsis |
| title_fullStr | Predictive value and regulatory mechanism of serum miR-499a-5p on myocardial dysfunction in sepsis |
| title_full_unstemmed | Predictive value and regulatory mechanism of serum miR-499a-5p on myocardial dysfunction in sepsis |
| title_short | Predictive value and regulatory mechanism of serum miR-499a-5p on myocardial dysfunction in sepsis |
| title_sort | predictive value and regulatory mechanism of serum mir 499a 5p on myocardial dysfunction in sepsis |
| topic | Sepsis Myocardial dysfunction miR-499a-5p EIF4E CK-MB BNP |
| url | https://doi.org/10.1186/s13019-021-01679-5 |
| work_keys_str_mv | AT chuangyang predictivevalueandregulatorymechanismofserummir499a5ponmyocardialdysfunctioninsepsis AT kunwen predictivevalueandregulatorymechanismofserummir499a5ponmyocardialdysfunctioninsepsis |