Acetylation of Phenylalanine Hydroxylase and Tryptophan 2,3-Dioxygenase Alters Hepatic Aromatic Amino Acid Metabolism in Weaned Piglets
Weaning significantly alters hepatic aromatic amino acid (AAA) metabolism and physiological functions. However, less is known about the regulating mechanism of hepatic AAA metabolism after weaning. A total of 200 21-day-old piglets (Duroc × Landrace) were assigned randomly to the control group and t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/10/4/146 |
| _version_ | 1797571086944043008 |
|---|---|
| author | Lu Huang Weilei Yao Tongxin Wang Juan Li Qiongyu He Feiruo Huang |
| author_facet | Lu Huang Weilei Yao Tongxin Wang Juan Li Qiongyu He Feiruo Huang |
| author_sort | Lu Huang |
| collection | DOAJ |
| description | Weaning significantly alters hepatic aromatic amino acid (AAA) metabolism and physiological functions. However, less is known about the regulating mechanism of hepatic AAA metabolism after weaning. A total of 200 21-day-old piglets (Duroc × Landrace) were assigned randomly to the control group and the weaning group. In this study, weaning significantly decreased the concentration of phenylalanine, tryptophan, and tyrosine in piglet livers (<i>p</i> < 0.05). Additionally, through the detection of liver AAA metabolites and metabolic enzyme activity, it was observed that hepatic tryptophan catabolism was enhanced, while that of phenylalanine was weakened (<i>p</i> < 0.05). Intriguingly, acetyl-proteome profiling of liver from weaned piglets showed that weaning exacerbated the acetylation of phenylalanine hydroxylase (PAH) and the deacetylation of tryptophan 2,3-dioxygenase (TDO). Analysis of PAH and TDO acetylation in Chang liver cells showed that acetylation decreased the PAH activity, while deacetylation increased the TDO activity (<i>p</i> < 0.05). Furthermore, metabolites of AAAs and the acetylation statuses of PAH and TDO in primary hepatocytes from weaned piglets were consistent with the results <i>in vivo</i>. These findings indicated that weaning altered the PAH and TDO activity by affecting the acetylation state of the enzyme in piglets’’ livers. Lysine acetylation may be a potential regulatory mechanism for AAA metabolism in response to weaning. |
| first_indexed | 2024-03-10T20:34:31Z |
| format | Article |
| id | doaj.art-15a987bf507843a5861e374b5860e5f5 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T20:34:31Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-15a987bf507843a5861e374b5860e5f52023-11-19T21:06:53ZengMDPI AGMetabolites2218-19892020-04-0110414610.3390/metabo10040146Acetylation of Phenylalanine Hydroxylase and Tryptophan 2,3-Dioxygenase Alters Hepatic Aromatic Amino Acid Metabolism in Weaned PigletsLu Huang0Weilei Yao1Tongxin Wang2Juan Li3Qiongyu He4Feiruo Huang5Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaWeaning significantly alters hepatic aromatic amino acid (AAA) metabolism and physiological functions. However, less is known about the regulating mechanism of hepatic AAA metabolism after weaning. A total of 200 21-day-old piglets (Duroc × Landrace) were assigned randomly to the control group and the weaning group. In this study, weaning significantly decreased the concentration of phenylalanine, tryptophan, and tyrosine in piglet livers (<i>p</i> < 0.05). Additionally, through the detection of liver AAA metabolites and metabolic enzyme activity, it was observed that hepatic tryptophan catabolism was enhanced, while that of phenylalanine was weakened (<i>p</i> < 0.05). Intriguingly, acetyl-proteome profiling of liver from weaned piglets showed that weaning exacerbated the acetylation of phenylalanine hydroxylase (PAH) and the deacetylation of tryptophan 2,3-dioxygenase (TDO). Analysis of PAH and TDO acetylation in Chang liver cells showed that acetylation decreased the PAH activity, while deacetylation increased the TDO activity (<i>p</i> < 0.05). Furthermore, metabolites of AAAs and the acetylation statuses of PAH and TDO in primary hepatocytes from weaned piglets were consistent with the results <i>in vivo</i>. These findings indicated that weaning altered the PAH and TDO activity by affecting the acetylation state of the enzyme in piglets’’ livers. Lysine acetylation may be a potential regulatory mechanism for AAA metabolism in response to weaning.https://www.mdpi.com/2218-1989/10/4/146liverweaned pigletsaromatic amino acids metabolismphenylalanine hydroxylasetryptophan 2,3-dioxygenaseacetylation |
| spellingShingle | Lu Huang Weilei Yao Tongxin Wang Juan Li Qiongyu He Feiruo Huang Acetylation of Phenylalanine Hydroxylase and Tryptophan 2,3-Dioxygenase Alters Hepatic Aromatic Amino Acid Metabolism in Weaned Piglets Metabolites liver weaned piglets aromatic amino acids metabolism phenylalanine hydroxylase tryptophan 2,3-dioxygenase acetylation |
| title | Acetylation of Phenylalanine Hydroxylase and Tryptophan 2,3-Dioxygenase Alters Hepatic Aromatic Amino Acid Metabolism in Weaned Piglets |
| title_full | Acetylation of Phenylalanine Hydroxylase and Tryptophan 2,3-Dioxygenase Alters Hepatic Aromatic Amino Acid Metabolism in Weaned Piglets |
| title_fullStr | Acetylation of Phenylalanine Hydroxylase and Tryptophan 2,3-Dioxygenase Alters Hepatic Aromatic Amino Acid Metabolism in Weaned Piglets |
| title_full_unstemmed | Acetylation of Phenylalanine Hydroxylase and Tryptophan 2,3-Dioxygenase Alters Hepatic Aromatic Amino Acid Metabolism in Weaned Piglets |
| title_short | Acetylation of Phenylalanine Hydroxylase and Tryptophan 2,3-Dioxygenase Alters Hepatic Aromatic Amino Acid Metabolism in Weaned Piglets |
| title_sort | acetylation of phenylalanine hydroxylase and tryptophan 2 3 dioxygenase alters hepatic aromatic amino acid metabolism in weaned piglets |
| topic | liver weaned piglets aromatic amino acids metabolism phenylalanine hydroxylase tryptophan 2,3-dioxygenase acetylation |
| url | https://www.mdpi.com/2218-1989/10/4/146 |
| work_keys_str_mv | AT luhuang acetylationofphenylalaninehydroxylaseandtryptophan23dioxygenasealtershepaticaromaticaminoacidmetabolisminweanedpiglets AT weileiyao acetylationofphenylalaninehydroxylaseandtryptophan23dioxygenasealtershepaticaromaticaminoacidmetabolisminweanedpiglets AT tongxinwang acetylationofphenylalaninehydroxylaseandtryptophan23dioxygenasealtershepaticaromaticaminoacidmetabolisminweanedpiglets AT juanli acetylationofphenylalaninehydroxylaseandtryptophan23dioxygenasealtershepaticaromaticaminoacidmetabolisminweanedpiglets AT qiongyuhe acetylationofphenylalaninehydroxylaseandtryptophan23dioxygenasealtershepaticaromaticaminoacidmetabolisminweanedpiglets AT feiruohuang acetylationofphenylalaninehydroxylaseandtryptophan23dioxygenasealtershepaticaromaticaminoacidmetabolisminweanedpiglets |