High throughput mutagenesis and screening for yeast engineering
Abstract The eukaryotic yeast Saccharomyces cerevisiae is a model host utilized for whole cell biocatalytic conversions, protein evolution, and scientific inquiries into the pathogenesis of human disease. Over the past decade, the scale and pace of such studies has drastically increased alongside th...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | Journal of Biological Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13036-022-00315-7 |
| _version_ | 1797973544441741312 |
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| author | Kendreze Holland John Blazeck |
| author_facet | Kendreze Holland John Blazeck |
| author_sort | Kendreze Holland |
| collection | DOAJ |
| description | Abstract The eukaryotic yeast Saccharomyces cerevisiae is a model host utilized for whole cell biocatalytic conversions, protein evolution, and scientific inquiries into the pathogenesis of human disease. Over the past decade, the scale and pace of such studies has drastically increased alongside the advent of novel tools for both genome-wide studies and targeted genetic mutagenesis. In this review, we will detail past and present (e.g., CRISPR/Cas) genome-scale screening platforms, typically employed in the context of growth-based selections for improved whole cell phenotype or for mechanistic interrogations. We will further highlight recent advances that enable the rapid and often continuous evolution of biomolecules with improved function. Additionally, we will detail the corresponding advances in high throughput selection and screening strategies that are essential for assessing or isolating cellular and protein improvements. Finally, we will describe how future developments can continue to advance yeast high throughput engineering. |
| first_indexed | 2024-04-11T04:05:56Z |
| format | Article |
| id | doaj.art-15ab932079464ba19d8510df9e7a80e5 |
| institution | Directory Open Access Journal |
| issn | 1754-1611 |
| language | English |
| last_indexed | 2024-04-11T04:05:56Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj.art-15ab932079464ba19d8510df9e7a80e52023-01-01T12:21:14ZengBMCJournal of Biological Engineering1754-16112022-12-0116111210.1186/s13036-022-00315-7High throughput mutagenesis and screening for yeast engineeringKendreze Holland0John Blazeck1Department of Biomedical Engineering, Georgia Institute of Technology and Emory UniversityBioengineering Program, Georgia Institute of TechnologyAbstract The eukaryotic yeast Saccharomyces cerevisiae is a model host utilized for whole cell biocatalytic conversions, protein evolution, and scientific inquiries into the pathogenesis of human disease. Over the past decade, the scale and pace of such studies has drastically increased alongside the advent of novel tools for both genome-wide studies and targeted genetic mutagenesis. In this review, we will detail past and present (e.g., CRISPR/Cas) genome-scale screening platforms, typically employed in the context of growth-based selections for improved whole cell phenotype or for mechanistic interrogations. We will further highlight recent advances that enable the rapid and often continuous evolution of biomolecules with improved function. Additionally, we will detail the corresponding advances in high throughput selection and screening strategies that are essential for assessing or isolating cellular and protein improvements. Finally, we will describe how future developments can continue to advance yeast high throughput engineering.https://doi.org/10.1186/s13036-022-00315-7High throughputDirected evolutionSelection schemesS. cerevisiaeYeast-displayIn vivo mutagenesis |
| spellingShingle | Kendreze Holland John Blazeck High throughput mutagenesis and screening for yeast engineering Journal of Biological Engineering High throughput Directed evolution Selection schemes S. cerevisiae Yeast-display In vivo mutagenesis |
| title | High throughput mutagenesis and screening for yeast engineering |
| title_full | High throughput mutagenesis and screening for yeast engineering |
| title_fullStr | High throughput mutagenesis and screening for yeast engineering |
| title_full_unstemmed | High throughput mutagenesis and screening for yeast engineering |
| title_short | High throughput mutagenesis and screening for yeast engineering |
| title_sort | high throughput mutagenesis and screening for yeast engineering |
| topic | High throughput Directed evolution Selection schemes S. cerevisiae Yeast-display In vivo mutagenesis |
| url | https://doi.org/10.1186/s13036-022-00315-7 |
| work_keys_str_mv | AT kendrezeholland highthroughputmutagenesisandscreeningforyeastengineering AT johnblazeck highthroughputmutagenesisandscreeningforyeastengineering |