GENETIC DIFFERENTIATION BETWEEN NOBLE CRAYFISH, ASTACUS ASTACUS (L.), POPULATIONS DETECTED BY MICROSATELLITE LENGTH VARIATION IN THE RDNA ITS1 REGION
The Internal Transcribed Spacer (ITS) region of the nuclear ribosomal repeat was investigated in the search for a suitable genetic marker for population studies of the noble crayfish Astacus astacus (L.). DNA sequencing revealed the presence of two microsatellite insertions in ITS1. By designing hig...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2002-07-01
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| Series: | Knowledge and Management of Aquatic Ecosystems |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1051/kmae:2002082 |
| _version_ | 1818676868598988800 |
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| author | EDSMAN L. FARRIS J. S. KÄLLERSJÖ M. PRESTEGAARD T. |
| author_facet | EDSMAN L. FARRIS J. S. KÄLLERSJÖ M. PRESTEGAARD T. |
| author_sort | EDSMAN L. |
| collection | DOAJ |
| description | The Internal Transcribed Spacer (ITS) region of the nuclear ribosomal repeat was investigated in the search for a suitable genetic marker for population studies of the noble crayfish Astacus astacus (L.). DNA sequencing revealed the presence of two microsatellite insertions in ITS1. By designing highly specific PCR primers, adjacent to one of the insertions, we were able to use fragment analysis to explore the variation of the insertion in 642 specimens from 17 populations of crayfish from Sweden and former Yugoslavia. A new statistical test, the Population Divergence Test, was developed to assess statistical significance of divergence between populations. This test does not assume Mendelian inheritance. Our results demonstrate that different populations often produce characteristic fragment patterns, and that most, but not all, populations are genetically distinct, with high significance. The populations that cannot be significantly differentiated are situated in close geographic proximity to each other and belong to the same main river system, probably reflecting that these populations have had recent contact and that gene flow has occurred. |
| first_indexed | 2024-12-17T08:50:19Z |
| format | Article |
| id | doaj.art-15ac366aa395440d90172ff7f62c5a75 |
| institution | Directory Open Access Journal |
| issn | 1961-9502 |
| language | English |
| last_indexed | 2024-12-17T08:50:19Z |
| publishDate | 2002-07-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Knowledge and Management of Aquatic Ecosystems |
| spelling | doaj.art-15ac366aa395440d90172ff7f62c5a752022-12-21T21:56:06ZengEDP SciencesKnowledge and Management of Aquatic Ecosystems1961-95022002-07-01036769170610.1051/kmae:2002082kmae200236734GENETIC DIFFERENTIATION BETWEEN NOBLE CRAYFISH, ASTACUS ASTACUS (L.), POPULATIONS DETECTED BY MICROSATELLITE LENGTH VARIATION IN THE RDNA ITS1 REGIONEDSMAN L.FARRIS J. S.KÄLLERSJÖ M.PRESTEGAARD T.The Internal Transcribed Spacer (ITS) region of the nuclear ribosomal repeat was investigated in the search for a suitable genetic marker for population studies of the noble crayfish Astacus astacus (L.). DNA sequencing revealed the presence of two microsatellite insertions in ITS1. By designing highly specific PCR primers, adjacent to one of the insertions, we were able to use fragment analysis to explore the variation of the insertion in 642 specimens from 17 populations of crayfish from Sweden and former Yugoslavia. A new statistical test, the Population Divergence Test, was developed to assess statistical significance of divergence between populations. This test does not assume Mendelian inheritance. Our results demonstrate that different populations often produce characteristic fragment patterns, and that most, but not all, populations are genetically distinct, with high significance. The populations that cannot be significantly differentiated are situated in close geographic proximity to each other and belong to the same main river system, probably reflecting that these populations have had recent contact and that gene flow has occurred.http://dx.doi.org/10.1051/kmae:2002082genetic diversitypopulationscrayfishAstacus astacusmicrosatellitesITSconservationPopulation Divergence Test |
| spellingShingle | EDSMAN L. FARRIS J. S. KÄLLERSJÖ M. PRESTEGAARD T. GENETIC DIFFERENTIATION BETWEEN NOBLE CRAYFISH, ASTACUS ASTACUS (L.), POPULATIONS DETECTED BY MICROSATELLITE LENGTH VARIATION IN THE RDNA ITS1 REGION Knowledge and Management of Aquatic Ecosystems genetic diversity populations crayfish Astacus astacus microsatellites ITS conservation Population Divergence Test |
| title | GENETIC DIFFERENTIATION BETWEEN NOBLE CRAYFISH, ASTACUS ASTACUS (L.), POPULATIONS DETECTED BY MICROSATELLITE LENGTH VARIATION IN THE RDNA ITS1 REGION |
| title_full | GENETIC DIFFERENTIATION BETWEEN NOBLE CRAYFISH, ASTACUS ASTACUS (L.), POPULATIONS DETECTED BY MICROSATELLITE LENGTH VARIATION IN THE RDNA ITS1 REGION |
| title_fullStr | GENETIC DIFFERENTIATION BETWEEN NOBLE CRAYFISH, ASTACUS ASTACUS (L.), POPULATIONS DETECTED BY MICROSATELLITE LENGTH VARIATION IN THE RDNA ITS1 REGION |
| title_full_unstemmed | GENETIC DIFFERENTIATION BETWEEN NOBLE CRAYFISH, ASTACUS ASTACUS (L.), POPULATIONS DETECTED BY MICROSATELLITE LENGTH VARIATION IN THE RDNA ITS1 REGION |
| title_short | GENETIC DIFFERENTIATION BETWEEN NOBLE CRAYFISH, ASTACUS ASTACUS (L.), POPULATIONS DETECTED BY MICROSATELLITE LENGTH VARIATION IN THE RDNA ITS1 REGION |
| title_sort | genetic differentiation between noble crayfish astacus astacus l populations detected by microsatellite length variation in the rdna its1 region |
| topic | genetic diversity populations crayfish Astacus astacus microsatellites ITS conservation Population Divergence Test |
| url | http://dx.doi.org/10.1051/kmae:2002082 |
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