Kinetics and modeling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitro
Background: Taraxacum officinale G.H. Weber ex Wiggers is a wild plant used in folk medicine to treat several diseases owing to bioactive secondary metabolites present in its tissue. The accumulation of such molecules in plant cells can occur as a response against abiotic stress, but these metabolit...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-11-01
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| Series: | Electronic Journal of Biotechnology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345818300368 |
| _version_ | 1818033566132469760 |
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| author | María Eugenia Martínez Paola Poirrier Dirk Prüfer Christian Schulze Gronover Lorena Jorquera Perla Ferrer Katy Díaz Rolando Chamy |
| author_facet | María Eugenia Martínez Paola Poirrier Dirk Prüfer Christian Schulze Gronover Lorena Jorquera Perla Ferrer Katy Díaz Rolando Chamy |
| author_sort | María Eugenia Martínez |
| collection | DOAJ |
| description | Background: Taraxacum officinale G.H. Weber ex Wiggers is a wild plant used in folk medicine to treat several diseases owing to bioactive secondary metabolites present in its tissue. The accumulation of such molecules in plant cells can occur as a response against abiotic stress, but these metabolites are often deposited in low concentrations. For this reason, the use of a biotechnological approach to improve the yields of technologically interesting bioactive compounds such as anthocyanins is a compelling option. This work focuses on investigating the potential of in vitro T. officinale cultures as an anthocyanin source. Results: To demonstrate the suitability of anthocyanin induction and accumulation in calluses under specific conditions, anthocyanin was induced in the T. officinale callus. A specific medium of 5.5% sucrose supplemented with 6-benzylaminopurine /1-naphthaleneacetic acid in a 10:1 ratio was used to produce an anthocyanin yield of 1.23 mg g-1 fw. An in vitro dandelion callus line was established from this experiment. Five mathematical models were then used to objectively and predictably explain the growth of anthocyanin-induced calluses from T. officinale. Of these models, the Richards model offered the most suitable representation of anthocyanin callus growth in a solid medium and permitted the calculation of the corresponding kinetic parameters. Conclusions: The findings demonstrate the potential of an in vitro anthocyanin-induced callus line from T. officinale as an industrial anthocyanin source.How to cite: Martínez ME, Poirrier P, Prüfer D, et al. Kinetics and modelling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitro. Electron J Biotechnol 2018;36. https://doi.org/10.1016/j.ejbt.2018.08.006. Keywords: anthocyanins, bioactive chemicals, callus, Dandelion, folk medicine, hypocotyl explants, in vitro culture, in vitro modeling, modeling cell growth, phytochemicals, Taraxacum officinale |
| first_indexed | 2024-12-10T06:25:18Z |
| format | Article |
| id | doaj.art-15ad2032fa2041baa3c7add829797bac |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-10T06:25:18Z |
| publishDate | 2018-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-15ad2032fa2041baa3c7add829797bac2022-12-22T01:59:13ZengElsevierElectronic Journal of Biotechnology0717-34582018-11-01361523Kinetics and modeling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitroMaría Eugenia Martínez0Paola Poirrier1Dirk Prüfer2Christian Schulze Gronover3Lorena Jorquera4Perla Ferrer5Katy Díaz6Rolando Chamy7Escuela de Ingeniería Bioquímica, Facultad de Ingeniería, Pontificia Universidad Católica de Valparaíso, General Cruz 34, Valparaíso, Chile; Fraunhofer Chile Research Foundation – Centre for Systems Biotechnology (FCR-CSB), Mariano Sánchez Fontecilla 310, of 1401, Las Condes, Santiago de Chile, ChileEscuela de Ingeniería Bioquímica, Facultad de Ingeniería, Pontificia Universidad Católica de Valparaíso, General Cruz 34, Valparaíso, ChileWestphalian Wilhelms-University of Münster, Institute of Plant Biology and Biotechnology, Schlossplatz 8, D-48143 Münster, Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Schlossplatz 8, D-48143 Münster, GermanyWestphalian Wilhelms-University of Münster, Institute of Plant Biology and Biotechnology, Schlossplatz 8, D-48143 Münster, GermanyEscuela de Ingeniería en Construcción, Facultad de Ingeniería, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2147, Valparaíso, ChileNúcleo Biotecnología Curauma, Pontificia Universidad Católica de Valparaíso, Avenida Universidad 330, Curauma, Valparaíso, ChileDepartamento de Química, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile; Corresponding authors.Escuela de Ingeniería Bioquímica, Facultad de Ingeniería, Pontificia Universidad Católica de Valparaíso, General Cruz 34, Valparaíso, Chile; Corresponding authors.Background: Taraxacum officinale G.H. Weber ex Wiggers is a wild plant used in folk medicine to treat several diseases owing to bioactive secondary metabolites present in its tissue. The accumulation of such molecules in plant cells can occur as a response against abiotic stress, but these metabolites are often deposited in low concentrations. For this reason, the use of a biotechnological approach to improve the yields of technologically interesting bioactive compounds such as anthocyanins is a compelling option. This work focuses on investigating the potential of in vitro T. officinale cultures as an anthocyanin source. Results: To demonstrate the suitability of anthocyanin induction and accumulation in calluses under specific conditions, anthocyanin was induced in the T. officinale callus. A specific medium of 5.5% sucrose supplemented with 6-benzylaminopurine /1-naphthaleneacetic acid in a 10:1 ratio was used to produce an anthocyanin yield of 1.23 mg g-1 fw. An in vitro dandelion callus line was established from this experiment. Five mathematical models were then used to objectively and predictably explain the growth of anthocyanin-induced calluses from T. officinale. Of these models, the Richards model offered the most suitable representation of anthocyanin callus growth in a solid medium and permitted the calculation of the corresponding kinetic parameters. Conclusions: The findings demonstrate the potential of an in vitro anthocyanin-induced callus line from T. officinale as an industrial anthocyanin source.How to cite: Martínez ME, Poirrier P, Prüfer D, et al. Kinetics and modelling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitro. Electron J Biotechnol 2018;36. https://doi.org/10.1016/j.ejbt.2018.08.006. Keywords: anthocyanins, bioactive chemicals, callus, Dandelion, folk medicine, hypocotyl explants, in vitro culture, in vitro modeling, modeling cell growth, phytochemicals, Taraxacum officinalehttp://www.sciencedirect.com/science/article/pii/S0717345818300368 |
| spellingShingle | María Eugenia Martínez Paola Poirrier Dirk Prüfer Christian Schulze Gronover Lorena Jorquera Perla Ferrer Katy Díaz Rolando Chamy Kinetics and modeling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitro Electronic Journal of Biotechnology |
| title | Kinetics and modeling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitro |
| title_full | Kinetics and modeling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitro |
| title_fullStr | Kinetics and modeling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitro |
| title_full_unstemmed | Kinetics and modeling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitro |
| title_short | Kinetics and modeling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitro |
| title_sort | kinetics and modeling of cell growth for potential anthocyanin induction in cultures of taraxacum officinale g h weber ex wiggers dandelion in vitro |
| url | http://www.sciencedirect.com/science/article/pii/S0717345818300368 |
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