Human Embryonic Stem Cell–derived Neural Crest Cells Promote Sprouting and Motor Recovery Following Spinal Cord Injury in Adult Rats
Spinal cord injury results in irreversible tissue damage and permanent sensorimotor impairment. The development of novel therapeutic strategies that improve the life quality of affected individuals is therefore of paramount importance. Cell transplantation is a promising approach for spinal cord inj...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2021-01-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/0963689720988245 |
| _version_ | 1818590767709421568 |
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| author | Iwan Jones Liudmila N. Novikova Mikael Wiberg Leif Carlsson Lev N. Novikov |
| author_facet | Iwan Jones Liudmila N. Novikova Mikael Wiberg Leif Carlsson Lev N. Novikov |
| author_sort | Iwan Jones |
| collection | DOAJ |
| description | Spinal cord injury results in irreversible tissue damage and permanent sensorimotor impairment. The development of novel therapeutic strategies that improve the life quality of affected individuals is therefore of paramount importance. Cell transplantation is a promising approach for spinal cord injury treatment and the present study assesses the efficacy of human embryonic stem cell–derived neural crest cells as preclinical cell-based therapy candidates. The differentiated neural crest cells exhibited characteristic molecular signatures and produced a range of biologically active trophic factors that stimulated in vitro neurite outgrowth of rat primary dorsal root ganglia neurons. Transplantation of the neural crest cells into both acute and chronic rat cervical spinal cord injury models promoted remodeling of descending raphespinal projections and contributed to the partial recovery of forelimb motor function. The results achieved in this proof-of-concept study demonstrates that human embryonic stem cell–derived neural crest cells warrant further investigation as cell-based therapy candidates for the treatment of spinal cord injury. |
| first_indexed | 2024-12-16T10:01:47Z |
| format | Article |
| id | doaj.art-15ada2d4d5154de692419955ab6c2a27 |
| institution | Directory Open Access Journal |
| issn | 1555-3892 |
| language | English |
| last_indexed | 2024-12-16T10:01:47Z |
| publishDate | 2021-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-15ada2d4d5154de692419955ab6c2a272022-12-21T22:35:45ZengSAGE PublishingCell Transplantation1555-38922021-01-013010.1177/0963689720988245Human Embryonic Stem Cell–derived Neural Crest Cells Promote Sprouting and Motor Recovery Following Spinal Cord Injury in Adult RatsIwan Jones0Liudmila N. Novikova1Mikael Wiberg2Leif Carlsson3Lev N. Novikov4 Department of Integrative Medical Biology, Umeå University, Umeå, Sweden Department of Integrative Medical Biology, Umeå University, Umeå, Sweden Department of Surgical and Perioperative Science, Section of Hand and Plastic Surgery, Umeå University, Umeå, Sweden , Umeå University, Umeå, Sweden Department of Integrative Medical Biology, Umeå University, Umeå, SwedenSpinal cord injury results in irreversible tissue damage and permanent sensorimotor impairment. The development of novel therapeutic strategies that improve the life quality of affected individuals is therefore of paramount importance. Cell transplantation is a promising approach for spinal cord injury treatment and the present study assesses the efficacy of human embryonic stem cell–derived neural crest cells as preclinical cell-based therapy candidates. The differentiated neural crest cells exhibited characteristic molecular signatures and produced a range of biologically active trophic factors that stimulated in vitro neurite outgrowth of rat primary dorsal root ganglia neurons. Transplantation of the neural crest cells into both acute and chronic rat cervical spinal cord injury models promoted remodeling of descending raphespinal projections and contributed to the partial recovery of forelimb motor function. The results achieved in this proof-of-concept study demonstrates that human embryonic stem cell–derived neural crest cells warrant further investigation as cell-based therapy candidates for the treatment of spinal cord injury.https://doi.org/10.1177/0963689720988245 |
| spellingShingle | Iwan Jones Liudmila N. Novikova Mikael Wiberg Leif Carlsson Lev N. Novikov Human Embryonic Stem Cell–derived Neural Crest Cells Promote Sprouting and Motor Recovery Following Spinal Cord Injury in Adult Rats Cell Transplantation |
| title | Human Embryonic Stem Cell–derived Neural Crest Cells Promote Sprouting and Motor Recovery Following Spinal Cord Injury in Adult Rats |
| title_full | Human Embryonic Stem Cell–derived Neural Crest Cells Promote Sprouting and Motor Recovery Following Spinal Cord Injury in Adult Rats |
| title_fullStr | Human Embryonic Stem Cell–derived Neural Crest Cells Promote Sprouting and Motor Recovery Following Spinal Cord Injury in Adult Rats |
| title_full_unstemmed | Human Embryonic Stem Cell–derived Neural Crest Cells Promote Sprouting and Motor Recovery Following Spinal Cord Injury in Adult Rats |
| title_short | Human Embryonic Stem Cell–derived Neural Crest Cells Promote Sprouting and Motor Recovery Following Spinal Cord Injury in Adult Rats |
| title_sort | human embryonic stem cell derived neural crest cells promote sprouting and motor recovery following spinal cord injury in adult rats |
| url | https://doi.org/10.1177/0963689720988245 |
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