The Sulfide-Responsive SqrR/BigR Homologous Regulator YgaV of <i>Escherichia coli</i> Controls Expression of Anaerobic Respiratory Genes and Antibiotic Tolerance
Compositions and activities of bacterial flora in the gastrointestinal tract significantly influence the metabolism, health, and disease of host humans and animals. These enteric bacteria can switch between aerobic and anaerobic growth if oxygen tension becomes limited. Interestingly, the switching...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/12/2359 |
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| author | Rajalakshmi Balasubramanian Koichi Hori Takayuki Shimizu Shingo Kasamatsu Kae Okamura Kan Tanaka Hideshi Ihara Shinji Masuda |
| author_facet | Rajalakshmi Balasubramanian Koichi Hori Takayuki Shimizu Shingo Kasamatsu Kae Okamura Kan Tanaka Hideshi Ihara Shinji Masuda |
| author_sort | Rajalakshmi Balasubramanian |
| collection | DOAJ |
| description | Compositions and activities of bacterial flora in the gastrointestinal tract significantly influence the metabolism, health, and disease of host humans and animals. These enteric bacteria can switch between aerobic and anaerobic growth if oxygen tension becomes limited. Interestingly, the switching mechanism is important for preventing reactive oxygen species (ROS) production and antibiotic tolerance. Studies have also shown that intracellular and extracellular sulfide molecules are involved in this switching control, although the mechanism is not fully clarified. Here, we found that YgaV, a sulfide-responsive transcription factor SqrR/BigR homolog, responded to sulfide compounds in vivo and in vitro to control anaerobic respiratory gene expression. YgaV also responded to H<sub>2</sub>O<sub>2</sub> scavenging in the enteric bacterium <i>Escherichia coli</i>. Although the wild-type (WT) showed increased antibiotic tolerance under H<sub>2</sub>S-atmospheric conditions, the <i>ygaV</i> mutant did not show such a phenotype. Additionally, antibiotic sensitivity was higher in the mutant than in the WT of both types in the presence and absence of exogenous H<sub>2</sub>S. These results, therefore, indicated that YgaV-dependent transcriptional regulation was responsible for maintaining redox homeostasis, ROS scavenging, and antibiotic tolerance. |
| first_indexed | 2024-03-09T17:23:42Z |
| format | Article |
| id | doaj.art-15ae9515b460483dbea4541ee4cb5cdf |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T17:23:42Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj.art-15ae9515b460483dbea4541ee4cb5cdf2023-11-24T12:56:43ZengMDPI AGAntioxidants2076-39212022-11-011112235910.3390/antiox11122359The Sulfide-Responsive SqrR/BigR Homologous Regulator YgaV of <i>Escherichia coli</i> Controls Expression of Anaerobic Respiratory Genes and Antibiotic ToleranceRajalakshmi Balasubramanian0Koichi Hori1Takayuki Shimizu2Shingo Kasamatsu3Kae Okamura4Kan Tanaka5Hideshi Ihara6Shinji Masuda7Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, JapanDepartment of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, JapanGraduate School of Arts and Sciences, The University of Tokyo, Tokyo 153-8902, JapanDepartment of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, Osaka 599-8531, JapanDepartment of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, Osaka 599-8531, JapanLaboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8501, JapanDepartment of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, Osaka 599-8531, JapanDepartment of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, JapanCompositions and activities of bacterial flora in the gastrointestinal tract significantly influence the metabolism, health, and disease of host humans and animals. These enteric bacteria can switch between aerobic and anaerobic growth if oxygen tension becomes limited. Interestingly, the switching mechanism is important for preventing reactive oxygen species (ROS) production and antibiotic tolerance. Studies have also shown that intracellular and extracellular sulfide molecules are involved in this switching control, although the mechanism is not fully clarified. Here, we found that YgaV, a sulfide-responsive transcription factor SqrR/BigR homolog, responded to sulfide compounds in vivo and in vitro to control anaerobic respiratory gene expression. YgaV also responded to H<sub>2</sub>O<sub>2</sub> scavenging in the enteric bacterium <i>Escherichia coli</i>. Although the wild-type (WT) showed increased antibiotic tolerance under H<sub>2</sub>S-atmospheric conditions, the <i>ygaV</i> mutant did not show such a phenotype. Additionally, antibiotic sensitivity was higher in the mutant than in the WT of both types in the presence and absence of exogenous H<sub>2</sub>S. These results, therefore, indicated that YgaV-dependent transcriptional regulation was responsible for maintaining redox homeostasis, ROS scavenging, and antibiotic tolerance.https://www.mdpi.com/2076-3921/11/12/2359antibiotics<i>Escherichia coli</i>hydrogen sulfidereactive sulfur speciesSqrRYgaV |
| spellingShingle | Rajalakshmi Balasubramanian Koichi Hori Takayuki Shimizu Shingo Kasamatsu Kae Okamura Kan Tanaka Hideshi Ihara Shinji Masuda The Sulfide-Responsive SqrR/BigR Homologous Regulator YgaV of <i>Escherichia coli</i> Controls Expression of Anaerobic Respiratory Genes and Antibiotic Tolerance Antioxidants antibiotics <i>Escherichia coli</i> hydrogen sulfide reactive sulfur species SqrR YgaV |
| title | The Sulfide-Responsive SqrR/BigR Homologous Regulator YgaV of <i>Escherichia coli</i> Controls Expression of Anaerobic Respiratory Genes and Antibiotic Tolerance |
| title_full | The Sulfide-Responsive SqrR/BigR Homologous Regulator YgaV of <i>Escherichia coli</i> Controls Expression of Anaerobic Respiratory Genes and Antibiotic Tolerance |
| title_fullStr | The Sulfide-Responsive SqrR/BigR Homologous Regulator YgaV of <i>Escherichia coli</i> Controls Expression of Anaerobic Respiratory Genes and Antibiotic Tolerance |
| title_full_unstemmed | The Sulfide-Responsive SqrR/BigR Homologous Regulator YgaV of <i>Escherichia coli</i> Controls Expression of Anaerobic Respiratory Genes and Antibiotic Tolerance |
| title_short | The Sulfide-Responsive SqrR/BigR Homologous Regulator YgaV of <i>Escherichia coli</i> Controls Expression of Anaerobic Respiratory Genes and Antibiotic Tolerance |
| title_sort | sulfide responsive sqrr bigr homologous regulator ygav of i escherichia coli i controls expression of anaerobic respiratory genes and antibiotic tolerance |
| topic | antibiotics <i>Escherichia coli</i> hydrogen sulfide reactive sulfur species SqrR YgaV |
| url | https://www.mdpi.com/2076-3921/11/12/2359 |
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