Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo.
Clostridium difficile is the principal cause of antibiotic-associated diarrhea. Major metabolic requirements for colonization and expansion of C. difficile after microbiota disturbance have not been fully determined. In this study, we show that glutamate utilization is important for C. difficile to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4965041?pdf=render |
| _version_ | 1811267357610344448 |
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| author | Brintha Parasumanna Girinathan Sterling Braun Apoorva Reddy Sirigireddy Jose Espinola Lopez Revathi Govind |
| author_facet | Brintha Parasumanna Girinathan Sterling Braun Apoorva Reddy Sirigireddy Jose Espinola Lopez Revathi Govind |
| author_sort | Brintha Parasumanna Girinathan |
| collection | DOAJ |
| description | Clostridium difficile is the principal cause of antibiotic-associated diarrhea. Major metabolic requirements for colonization and expansion of C. difficile after microbiota disturbance have not been fully determined. In this study, we show that glutamate utilization is important for C. difficile to establish itself in the animal gut. When the gluD gene, which codes for glutamate dehydrogenase (GDH), was disrupted, the mutant C. difficile was unable to colonize and cause disease in a hamster model. Further, from the complementation experiment it appears that extracellular GDH may be playing a role in promoting C. difficile colonization and disease progression. Quantification of free amino acids in the hamster gut during C. difficile infection showed that glutamate is among preferred amino acids utilized by C. difficile during its expansion. This study provides evidence of the importance of glutamate metabolism for C. difficile pathogenesis. |
| first_indexed | 2024-04-12T20:59:56Z |
| format | Article |
| id | doaj.art-15b39da477e24b55b0f6db7e3f0ec1b2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T20:59:56Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-15b39da477e24b55b0f6db7e3f0ec1b22022-12-22T03:16:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01117e016010710.1371/journal.pone.0160107Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo.Brintha Parasumanna GirinathanSterling BraunApoorva Reddy SirigireddyJose Espinola LopezRevathi GovindClostridium difficile is the principal cause of antibiotic-associated diarrhea. Major metabolic requirements for colonization and expansion of C. difficile after microbiota disturbance have not been fully determined. In this study, we show that glutamate utilization is important for C. difficile to establish itself in the animal gut. When the gluD gene, which codes for glutamate dehydrogenase (GDH), was disrupted, the mutant C. difficile was unable to colonize and cause disease in a hamster model. Further, from the complementation experiment it appears that extracellular GDH may be playing a role in promoting C. difficile colonization and disease progression. Quantification of free amino acids in the hamster gut during C. difficile infection showed that glutamate is among preferred amino acids utilized by C. difficile during its expansion. This study provides evidence of the importance of glutamate metabolism for C. difficile pathogenesis.http://europepmc.org/articles/PMC4965041?pdf=render |
| spellingShingle | Brintha Parasumanna Girinathan Sterling Braun Apoorva Reddy Sirigireddy Jose Espinola Lopez Revathi Govind Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo. PLoS ONE |
| title | Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo. |
| title_full | Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo. |
| title_fullStr | Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo. |
| title_full_unstemmed | Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo. |
| title_short | Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo. |
| title_sort | importance of glutamate dehydrogenase gdh in clostridium difficile colonization in vivo |
| url | http://europepmc.org/articles/PMC4965041?pdf=render |
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