Structure of O-Polysaccharide and Lipid A of <i>Pantoea Agglomerans</i> 8488
The <i>Pantoea agglomerans</i> 8488 lipopolysaccharide (LPS) was isolated, purified and characterized by monosaccharide and fatty acid analysis. The O-polysaccharide and lipid A components of the LPS were separated by mild acid degradation. Lipid A was studied by electrospray ionization...
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MDPI AG
2020-05-01
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| Series: | Biomolecules |
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| author | Tetiana V. Bulyhina Evelina L. Zdorovenko Ludmila D. Varbanets Alexander S. Shashkov Alexandra A. Kadykova Yuriy A. Knirel Oleh V. Lushchak |
| author_facet | Tetiana V. Bulyhina Evelina L. Zdorovenko Ludmila D. Varbanets Alexander S. Shashkov Alexandra A. Kadykova Yuriy A. Knirel Oleh V. Lushchak |
| author_sort | Tetiana V. Bulyhina |
| collection | DOAJ |
| description | The <i>Pantoea agglomerans</i> 8488 lipopolysaccharide (LPS) was isolated, purified and characterized by monosaccharide and fatty acid analysis. The O-polysaccharide and lipid A components of the LPS were separated by mild acid degradation. Lipid A was studied by electrospray ionization mass spectrometry (ESI-MS) and found to consist of hexa-, penta-, tetra- and tri-acylated species. Two-dimensional nuclear magnetic resonance (NMR) spectroscopy revealed the following structure of the O-polysaccharide repeating unit →3)-α-L-Rha<i>p</i>-(1→6)-α-D-Man<i>p</i>-(1→3)-α-L-Fuc<i>p</i>-(1→3)-β-D-GlcNAc<i>p</i>-(1→. The LPS showed a low level of toxicity, was not pyrogenic, and reduced the adhesiveness index of microorganisms to 2.12, which was twofold less than the control. LPS modified by complex compounds of germanium (IV) and tin (IV) were obtained. It was found that six LPS samples modified by Sn compounds and two LPS samples modified by Ge compounds lost their toxic activity when administered to mice in a dose of LD<sub>50</sub> (105 µg/mice or 5 mg/kg). However, none of the modified LPS samples changed their serological activity in an Ouchterlony double immunodiffusion test in agar. |
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| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T19:38:20Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-15b3c69ad71440bb8fd255bfd0f59cb22023-11-20T01:25:55ZengMDPI AGBiomolecules2218-273X2020-05-0110580410.3390/biom10050804Structure of O-Polysaccharide and Lipid A of <i>Pantoea Agglomerans</i> 8488Tetiana V. Bulyhina0Evelina L. Zdorovenko1Ludmila D. Varbanets2Alexander S. Shashkov3Alexandra A. Kadykova4Yuriy A. Knirel5Oleh V. Lushchak6D.K. Zabolotny Institute of Microbiology and Virology (IMV), The National Academy of Sciences, 154 Zabolotnoho Str., 03143 Kyiv, UkraineN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, RussiaN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, RussiaN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, RussiaHigher Chemical College of the Russian Academy of Sciences, D.I. Mendeleev University of Chemical Technology of Russia, Moscow 125047, RussiaN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, RussiaDepartment of Biochemistry and Biotechnology, Natural Sciences Institute, Vasyl Stefanyk Precarpathian National University, 76018 Ivano-Frankivsk, UkraineThe <i>Pantoea agglomerans</i> 8488 lipopolysaccharide (LPS) was isolated, purified and characterized by monosaccharide and fatty acid analysis. The O-polysaccharide and lipid A components of the LPS were separated by mild acid degradation. Lipid A was studied by electrospray ionization mass spectrometry (ESI-MS) and found to consist of hexa-, penta-, tetra- and tri-acylated species. Two-dimensional nuclear magnetic resonance (NMR) spectroscopy revealed the following structure of the O-polysaccharide repeating unit →3)-α-L-Rha<i>p</i>-(1→6)-α-D-Man<i>p</i>-(1→3)-α-L-Fuc<i>p</i>-(1→3)-β-D-GlcNAc<i>p</i>-(1→. The LPS showed a low level of toxicity, was not pyrogenic, and reduced the adhesiveness index of microorganisms to 2.12, which was twofold less than the control. LPS modified by complex compounds of germanium (IV) and tin (IV) were obtained. It was found that six LPS samples modified by Sn compounds and two LPS samples modified by Ge compounds lost their toxic activity when administered to mice in a dose of LD<sub>50</sub> (105 µg/mice or 5 mg/kg). However, none of the modified LPS samples changed their serological activity in an Ouchterlony double immunodiffusion test in agar.https://www.mdpi.com/2218-273X/10/5/804<i>Pantoea agglomerans</i>lipopolysaccharideO-polysaccharide structurelipid Atoxicitypyrogenicity |
| spellingShingle | Tetiana V. Bulyhina Evelina L. Zdorovenko Ludmila D. Varbanets Alexander S. Shashkov Alexandra A. Kadykova Yuriy A. Knirel Oleh V. Lushchak Structure of O-Polysaccharide and Lipid A of <i>Pantoea Agglomerans</i> 8488 Biomolecules <i>Pantoea agglomerans</i> lipopolysaccharide O-polysaccharide structure lipid A toxicity pyrogenicity |
| title | Structure of O-Polysaccharide and Lipid A of <i>Pantoea Agglomerans</i> 8488 |
| title_full | Structure of O-Polysaccharide and Lipid A of <i>Pantoea Agglomerans</i> 8488 |
| title_fullStr | Structure of O-Polysaccharide and Lipid A of <i>Pantoea Agglomerans</i> 8488 |
| title_full_unstemmed | Structure of O-Polysaccharide and Lipid A of <i>Pantoea Agglomerans</i> 8488 |
| title_short | Structure of O-Polysaccharide and Lipid A of <i>Pantoea Agglomerans</i> 8488 |
| title_sort | structure of o polysaccharide and lipid a of i pantoea agglomerans i 8488 |
| topic | <i>Pantoea agglomerans</i> lipopolysaccharide O-polysaccharide structure lipid A toxicity pyrogenicity |
| url | https://www.mdpi.com/2218-273X/10/5/804 |
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