Involvement of DR→mPFC 5-HTergic neural projections in changes of social exploration behaviors caused by adult chronic social isolation in mice
Social contacts play an important role in the development and survival of social animals. Social isolation (SI) at adolescence often induces abnormalities in many kinds of behaviors. This study assessed whether five weeks of continuous SI at adulthood could alter social behaviors and whether dorsal...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-08-01
|
| Series: | Brain Research Bulletin |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0361923022001137 |
| _version_ | 1797367802022068224 |
|---|---|
| author | Zijian Lv Yuting Zhang Lizi Zhang Wenjuan Hou Miao Lin Yufeng Xun Huan Ma Yin Li Yitong Li Fadao Tai Zhixiong He |
| author_facet | Zijian Lv Yuting Zhang Lizi Zhang Wenjuan Hou Miao Lin Yufeng Xun Huan Ma Yin Li Yitong Li Fadao Tai Zhixiong He |
| author_sort | Zijian Lv |
| collection | DOAJ |
| description | Social contacts play an important role in the development and survival of social animals. Social isolation (SI) at adolescence often induces abnormalities in many kinds of behaviors. This study assessed whether five weeks of continuous SI at adulthood could alter social behaviors and whether dorsal raphe nucleus (DR) to medial prefrontal cortex (mPFC) 5-HT neural projections were involved in this alteration in C57BL/6J adult male mice. The present study found that five weeks chronic social isolation (CSI) at adulthood increased mounting and sniffing behaviors in resident-intruder test, and lengthened duration staying in interaction zone of stranger cage in the three-chamber social preference test. CSI also reduced the release of 5-HT in the mPFC detected by 5-HT 1.0 sensor and measured by in vivo fiber photometry test. Meanwhile, the c-Fos expression indicated that CSI reduced the activity of serotonergic neurons. Chemogenetic activation of DR-mPFC 5-HTergic projection reduced sniffing of CSI mice in the resident-intruder test, but didn’t significantly affect mounting behavior. It also decreased the interaction time during the three-chamber social preference test. Thus, 5-HT neural projections from the DR to the mPFC are involved in changes of social exploration behaviors induced by CSI at adulthood. |
| first_indexed | 2024-03-08T17:22:24Z |
| format | Article |
| id | doaj.art-15b4a0c2eb4c439b897db9b2ef5cf59d |
| institution | Directory Open Access Journal |
| issn | 1873-2747 |
| language | English |
| last_indexed | 2024-03-08T17:22:24Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain Research Bulletin |
| spelling | doaj.art-15b4a0c2eb4c439b897db9b2ef5cf59d2024-01-03T04:11:21ZengElsevierBrain Research Bulletin1873-27472022-08-011861626Involvement of DR→mPFC 5-HTergic neural projections in changes of social exploration behaviors caused by adult chronic social isolation in miceZijian Lv0Yuting Zhang1Lizi Zhang2Wenjuan Hou3Miao Lin4Yufeng Xun5Huan Ma6Yin Li7Yitong Li8Fadao Tai9Zhixiong He10Institute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaInstitute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaInstitute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaInstitute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaInstitute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaInstitute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaInstitute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaInstitute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaInstitute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaCorrespondence to: 620 Xi Chang’ an Street, Chang’ an District, Xi’an city, Shaanxi province, China.; Institute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaCorrespondence to: 620 Xi Chang’ an Street, Chang’ an District, Xi’an city, Shaanxi province, China.; Institute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaSocial contacts play an important role in the development and survival of social animals. Social isolation (SI) at adolescence often induces abnormalities in many kinds of behaviors. This study assessed whether five weeks of continuous SI at adulthood could alter social behaviors and whether dorsal raphe nucleus (DR) to medial prefrontal cortex (mPFC) 5-HT neural projections were involved in this alteration in C57BL/6J adult male mice. The present study found that five weeks chronic social isolation (CSI) at adulthood increased mounting and sniffing behaviors in resident-intruder test, and lengthened duration staying in interaction zone of stranger cage in the three-chamber social preference test. CSI also reduced the release of 5-HT in the mPFC detected by 5-HT 1.0 sensor and measured by in vivo fiber photometry test. Meanwhile, the c-Fos expression indicated that CSI reduced the activity of serotonergic neurons. Chemogenetic activation of DR-mPFC 5-HTergic projection reduced sniffing of CSI mice in the resident-intruder test, but didn’t significantly affect mounting behavior. It also decreased the interaction time during the three-chamber social preference test. Thus, 5-HT neural projections from the DR to the mPFC are involved in changes of social exploration behaviors induced by CSI at adulthood.http://www.sciencedirect.com/science/article/pii/S0361923022001137Chronic social isolationSerotoninDorsal rapheMedial prefrontal cortexSociability |
| spellingShingle | Zijian Lv Yuting Zhang Lizi Zhang Wenjuan Hou Miao Lin Yufeng Xun Huan Ma Yin Li Yitong Li Fadao Tai Zhixiong He Involvement of DR→mPFC 5-HTergic neural projections in changes of social exploration behaviors caused by adult chronic social isolation in mice Brain Research Bulletin Chronic social isolation Serotonin Dorsal raphe Medial prefrontal cortex Sociability |
| title | Involvement of DR→mPFC 5-HTergic neural projections in changes of social exploration behaviors caused by adult chronic social isolation in mice |
| title_full | Involvement of DR→mPFC 5-HTergic neural projections in changes of social exploration behaviors caused by adult chronic social isolation in mice |
| title_fullStr | Involvement of DR→mPFC 5-HTergic neural projections in changes of social exploration behaviors caused by adult chronic social isolation in mice |
| title_full_unstemmed | Involvement of DR→mPFC 5-HTergic neural projections in changes of social exploration behaviors caused by adult chronic social isolation in mice |
| title_short | Involvement of DR→mPFC 5-HTergic neural projections in changes of social exploration behaviors caused by adult chronic social isolation in mice |
| title_sort | involvement of dr mpfc 5 htergic neural projections in changes of social exploration behaviors caused by adult chronic social isolation in mice |
| topic | Chronic social isolation Serotonin Dorsal raphe Medial prefrontal cortex Sociability |
| url | http://www.sciencedirect.com/science/article/pii/S0361923022001137 |
| work_keys_str_mv | AT zijianlv involvementofdrmpfc5htergicneuralprojectionsinchangesofsocialexplorationbehaviorscausedbyadultchronicsocialisolationinmice AT yutingzhang involvementofdrmpfc5htergicneuralprojectionsinchangesofsocialexplorationbehaviorscausedbyadultchronicsocialisolationinmice AT lizizhang involvementofdrmpfc5htergicneuralprojectionsinchangesofsocialexplorationbehaviorscausedbyadultchronicsocialisolationinmice AT wenjuanhou involvementofdrmpfc5htergicneuralprojectionsinchangesofsocialexplorationbehaviorscausedbyadultchronicsocialisolationinmice AT miaolin involvementofdrmpfc5htergicneuralprojectionsinchangesofsocialexplorationbehaviorscausedbyadultchronicsocialisolationinmice AT yufengxun involvementofdrmpfc5htergicneuralprojectionsinchangesofsocialexplorationbehaviorscausedbyadultchronicsocialisolationinmice AT huanma involvementofdrmpfc5htergicneuralprojectionsinchangesofsocialexplorationbehaviorscausedbyadultchronicsocialisolationinmice AT yinli involvementofdrmpfc5htergicneuralprojectionsinchangesofsocialexplorationbehaviorscausedbyadultchronicsocialisolationinmice AT yitongli involvementofdrmpfc5htergicneuralprojectionsinchangesofsocialexplorationbehaviorscausedbyadultchronicsocialisolationinmice AT fadaotai involvementofdrmpfc5htergicneuralprojectionsinchangesofsocialexplorationbehaviorscausedbyadultchronicsocialisolationinmice AT zhixionghe involvementofdrmpfc5htergicneuralprojectionsinchangesofsocialexplorationbehaviorscausedbyadultchronicsocialisolationinmice |