Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

Abstract Single-chain variable fragments (scFvs) have gained increased attention among researchers in both academic and industrial fields owing to simple production in E. coli. The E. coli periplasm has been the site of choice for the expression of scFv molecules due to its oxidizing milieu facilita...

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Bibliographic Details
Main Authors: Elham Ghamghami, Marjan Abri Aghdam, Mohammad Reza Tohidkia, Asadollah Ahmadikhah, Morteza Khanmohammadi, Tayebeh Mehdipour, Ahad Mokhtarzadeh, Behzad Baradaran
Format: Article
Language:English
Published: SpringerOpen 2020-07-01
Series:AMB Express
Subjects:
TES extraction
Periplasmic extraction
scFv
E. coli
Online Access:http://link.springer.com/article/10.1186/s13568-020-01063-x

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