LncRNA TERRA expression level and telomere length in placenta samples of healthy and preeclampsia women
Introduction: Preeclampsia is a specific pregnancy syndrome that affects all parts of the body. One of the possible causes of preeclampsia is genetic factors, including genetic predisposing genes. The aim of this study was to compare the expression level of LncRNA (large non coding RNA) TERRA (Telo...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Mashhad University of Medical Sciences
2020-05-01
|
| Series: | Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān |
| Subjects: | |
| Online Access: | https://ijogi.mums.ac.ir/article_15992_9fd6668e09bdc288ce6fe2004ea81534.pdf |
| _version_ | 1811233774167392256 |
|---|---|
| author | Giti Rahimi Seyed Saied Hosseini-Asl Maryam Marghoub Hamed Seif Elahi Firooz Amani Samira Shahbazzadegan |
| author_facet | Giti Rahimi Seyed Saied Hosseini-Asl Maryam Marghoub Hamed Seif Elahi Firooz Amani Samira Shahbazzadegan |
| author_sort | Giti Rahimi |
| collection | DOAJ |
| description | Introduction: Preeclampsia is a specific pregnancy syndrome that affects all parts of the body. One of the possible causes of preeclampsia is genetic factors, including genetic predisposing genes. The aim of this study was to compare the expression level of LncRNA (large non coding RNA) TERRA (Telomeric repeat-containing RNA) and telomere length in a pair of women with preeclampsia and healthy. Methods: In this case-control study which was performed in 2017-2018, placenta specimens were taken from 25 mothers with Pre-eclampsia who referred to Ardabil Alavi Hospital and were candidate for pregnancy termination; in the control group also, placenta specimens were taken from 25 women without preeclampsia. After extraction of RNA and cDNA synthesis, TERRA expression level was measured by Real Time PCR and the changes of Telomere length was measured by Telomere Length Assay technique. Data were analyzed by SPSS software (version 16) and Independent t-test. PResults: The mean for expression level of LNCRNA TERRA in the case group was 7.87 ± 1.9 and in the control group was 9.8 ± 2.8. Independent T-test results showed significant difference between the two groups in terms of expression level of LNCRNA TERRA (p=0.006). The mean for Telomere length in the case group was 4.35 ± 1.9 and in the control group was 6.2 ± 1.54. Independent T-test results showed significant difference between the two groups in terms of Telomere length, too (p=0.001). There was no significant correlation between maternal age and LNCRNA TERRA expression level and telomere length (p = 0.88). There was no statistically significant difference in expression level of LNCRNA TERRA and Telomere length between the sexes of newborns (p=0.1). Conclusion: Our data identified the significant role of LNCRNA TERRA and Telomere length in Pre-eclampsia. LNCRNA TERRA and Telomere length can be used to predict and diagnose preeclampsia |
| first_indexed | 2024-04-12T11:25:06Z |
| format | Article |
| id | doaj.art-15b63ac5608240eba5a3a80600777bca |
| institution | Directory Open Access Journal |
| issn | 1680-2993 2008-2363 |
| language | fas |
| last_indexed | 2024-04-12T11:25:06Z |
| publishDate | 2020-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān |
| spelling | doaj.art-15b63ac5608240eba5a3a80600777bca2022-12-22T03:35:14ZfasMashhad University of Medical SciencesMajallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān1680-29932008-23632020-05-0123391810.22038/ijogi.2020.1599215992LncRNA TERRA expression level and telomere length in placenta samples of healthy and preeclampsia womenGiti Rahimi0Seyed Saied Hosseini-Asl1Maryam Marghoub2Hamed Seif Elahi3Firooz Amani4Samira Shahbazzadegan5Associate Professor, Department of Obstetrics and Gynecology, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.Associate Professor, Department of Anatomy and Pathology, School of Medicine and Paramedical, Digestive Diseases Research Center, Ardabil University of Medical Sciences, Ardabil, Iran.Gynecologist, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.Student of Medicine, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.Associate Professor, Department of Biostatistics, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.Assistant Professor, Department of Midwifery, School of Nursing and Midwifery, Ardabil University of Medical Sciences, Ardabil, Iran.Introduction: Preeclampsia is a specific pregnancy syndrome that affects all parts of the body. One of the possible causes of preeclampsia is genetic factors, including genetic predisposing genes. The aim of this study was to compare the expression level of LncRNA (large non coding RNA) TERRA (Telomeric repeat-containing RNA) and telomere length in a pair of women with preeclampsia and healthy. Methods: In this case-control study which was performed in 2017-2018, placenta specimens were taken from 25 mothers with Pre-eclampsia who referred to Ardabil Alavi Hospital and were candidate for pregnancy termination; in the control group also, placenta specimens were taken from 25 women without preeclampsia. After extraction of RNA and cDNA synthesis, TERRA expression level was measured by Real Time PCR and the changes of Telomere length was measured by Telomere Length Assay technique. Data were analyzed by SPSS software (version 16) and Independent t-test. PResults: The mean for expression level of LNCRNA TERRA in the case group was 7.87 ± 1.9 and in the control group was 9.8 ± 2.8. Independent T-test results showed significant difference between the two groups in terms of expression level of LNCRNA TERRA (p=0.006). The mean for Telomere length in the case group was 4.35 ± 1.9 and in the control group was 6.2 ± 1.54. Independent T-test results showed significant difference between the two groups in terms of Telomere length, too (p=0.001). There was no significant correlation between maternal age and LNCRNA TERRA expression level and telomere length (p = 0.88). There was no statistically significant difference in expression level of LNCRNA TERRA and Telomere length between the sexes of newborns (p=0.1). Conclusion: Our data identified the significant role of LNCRNA TERRA and Telomere length in Pre-eclampsia. LNCRNA TERRA and Telomere length can be used to predict and diagnose preeclampsiahttps://ijogi.mums.ac.ir/article_15992_9fd6668e09bdc288ce6fe2004ea81534.pdfpre-eclampsiatelomere lengthterra expression |
| spellingShingle | Giti Rahimi Seyed Saied Hosseini-Asl Maryam Marghoub Hamed Seif Elahi Firooz Amani Samira Shahbazzadegan LncRNA TERRA expression level and telomere length in placenta samples of healthy and preeclampsia women Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān pre-eclampsia telomere length terra expression |
| title | LncRNA TERRA expression level and telomere length in placenta samples of healthy and preeclampsia women |
| title_full | LncRNA TERRA expression level and telomere length in placenta samples of healthy and preeclampsia women |
| title_fullStr | LncRNA TERRA expression level and telomere length in placenta samples of healthy and preeclampsia women |
| title_full_unstemmed | LncRNA TERRA expression level and telomere length in placenta samples of healthy and preeclampsia women |
| title_short | LncRNA TERRA expression level and telomere length in placenta samples of healthy and preeclampsia women |
| title_sort | lncrna terra expression level and telomere length in placenta samples of healthy and preeclampsia women |
| topic | pre-eclampsia telomere length terra expression |
| url | https://ijogi.mums.ac.ir/article_15992_9fd6668e09bdc288ce6fe2004ea81534.pdf |
| work_keys_str_mv | AT gitirahimi lncrnaterraexpressionlevelandtelomerelengthinplacentasamplesofhealthyandpreeclampsiawomen AT seyedsaiedhosseiniasl lncrnaterraexpressionlevelandtelomerelengthinplacentasamplesofhealthyandpreeclampsiawomen AT maryammarghoub lncrnaterraexpressionlevelandtelomerelengthinplacentasamplesofhealthyandpreeclampsiawomen AT hamedseifelahi lncrnaterraexpressionlevelandtelomerelengthinplacentasamplesofhealthyandpreeclampsiawomen AT firoozamani lncrnaterraexpressionlevelandtelomerelengthinplacentasamplesofhealthyandpreeclampsiawomen AT samirashahbazzadegan lncrnaterraexpressionlevelandtelomerelengthinplacentasamplesofhealthyandpreeclampsiawomen |