The Association between Maternal Oral Inflammation and Neutrophil Phenotypes and Poly-Unsaturated Fatty Acids Composition in Human Milk: A Prospective Cohort Study
This prospective cohort study aimed to investigate the impact of maternal oral inflammation on human milk composition including neutrophil counts, activation state (based on cluster of differentiation (CD) markers expression), and fatty acid levels. Fifty mothers were recruited from St. Michael’s ho...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/24/4110 |
| _version_ | 1827641370851934208 |
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| author | Rana Badewy Amir Azarpazhooh Howard Tenenbaum Kristin L. Connor Jim Yuan Lai Michael Sgro Richard P. Bazinet Noah Fine Erin Watson Chunxiang Sun Sourav Saha Michael Glogauer |
| author_facet | Rana Badewy Amir Azarpazhooh Howard Tenenbaum Kristin L. Connor Jim Yuan Lai Michael Sgro Richard P. Bazinet Noah Fine Erin Watson Chunxiang Sun Sourav Saha Michael Glogauer |
| author_sort | Rana Badewy |
| collection | DOAJ |
| description | This prospective cohort study aimed to investigate the impact of maternal oral inflammation on human milk composition including neutrophil counts, activation state (based on cluster of differentiation (CD) markers expression), and fatty acid levels. Fifty mothers were recruited from St. Michael’s hospital, Toronto, and followed up from 2–4 weeks until 4 months postpartum. Oral rinse and human milk samples were collected at both timepoints. Oral polymorphonuclear neutrophils (oPMNs) within the rinses were quantified using flow cytometry and the participants’ oral health state was categorized into three groups (i.e., healthy, moderate, and severe) based on the oPMNs counts. Fatty acids were identified and quantified using a gas chromatography-flame ionization detector (GC-FID). Compared to mothers with a healthy oral health state, mothers with moderate to severe oral inflammation had a statistically significant decrease in the expression of CD64 biomarker, an increase in the expression of CD14 biomarker on human milk neutrophils and a decrease in the levels of eicosapentaenoic acid (C20:5n-3) in their human milk at follow-up compared to baseline. This study demonstrates for the first time that maternal oral inflammation can affect human milk composition. The mechanism by which these alterations can affect infant health outcomes in the long term critically needs to be considered. |
| first_indexed | 2024-03-09T17:12:02Z |
| format | Article |
| id | doaj.art-15b9d922e1c6471fb2a794f9f3dd4f72 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T17:12:02Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-15b9d922e1c6471fb2a794f9f3dd4f722023-11-24T13:56:01ZengMDPI AGCells2073-44092022-12-011124411010.3390/cells11244110The Association between Maternal Oral Inflammation and Neutrophil Phenotypes and Poly-Unsaturated Fatty Acids Composition in Human Milk: A Prospective Cohort StudyRana Badewy0Amir Azarpazhooh1Howard Tenenbaum2Kristin L. Connor3Jim Yuan Lai4Michael Sgro5Richard P. Bazinet6Noah Fine7Erin Watson8Chunxiang Sun9Sourav Saha10Michael Glogauer11Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, CanadaFaculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, CanadaFaculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, CanadaDepartment of Health Sciences, Carleton University, Ottawa, ON K1S 5B6, CanadaFaculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, CanadaDepartment of Pediatrics, St. Michael’s Hospital, Unity Health Toronto, Toronto, ON M5B 1W8, CanadaDepartment of Nutritional Sciences, University of Toronto, Toronto, ON M5S 1A8, CanadaMatrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, CanadaDepartment of Dental Oncology, Princess Margaret Cancer Centre, Toronto, ON M5G 2C1, CanadaMatrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, CanadaMatrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, CanadaFaculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, CanadaThis prospective cohort study aimed to investigate the impact of maternal oral inflammation on human milk composition including neutrophil counts, activation state (based on cluster of differentiation (CD) markers expression), and fatty acid levels. Fifty mothers were recruited from St. Michael’s hospital, Toronto, and followed up from 2–4 weeks until 4 months postpartum. Oral rinse and human milk samples were collected at both timepoints. Oral polymorphonuclear neutrophils (oPMNs) within the rinses were quantified using flow cytometry and the participants’ oral health state was categorized into three groups (i.e., healthy, moderate, and severe) based on the oPMNs counts. Fatty acids were identified and quantified using a gas chromatography-flame ionization detector (GC-FID). Compared to mothers with a healthy oral health state, mothers with moderate to severe oral inflammation had a statistically significant decrease in the expression of CD64 biomarker, an increase in the expression of CD14 biomarker on human milk neutrophils and a decrease in the levels of eicosapentaenoic acid (C20:5n-3) in their human milk at follow-up compared to baseline. This study demonstrates for the first time that maternal oral inflammation can affect human milk composition. The mechanism by which these alterations can affect infant health outcomes in the long term critically needs to be considered.https://www.mdpi.com/2073-4409/11/24/4110neutrophilsbreast milkoral inflammatory loadperiodontal diseasesoral polymorphonuclear neutrophilshuman milk |
| spellingShingle | Rana Badewy Amir Azarpazhooh Howard Tenenbaum Kristin L. Connor Jim Yuan Lai Michael Sgro Richard P. Bazinet Noah Fine Erin Watson Chunxiang Sun Sourav Saha Michael Glogauer The Association between Maternal Oral Inflammation and Neutrophil Phenotypes and Poly-Unsaturated Fatty Acids Composition in Human Milk: A Prospective Cohort Study Cells neutrophils breast milk oral inflammatory load periodontal diseases oral polymorphonuclear neutrophils human milk |
| title | The Association between Maternal Oral Inflammation and Neutrophil Phenotypes and Poly-Unsaturated Fatty Acids Composition in Human Milk: A Prospective Cohort Study |
| title_full | The Association between Maternal Oral Inflammation and Neutrophil Phenotypes and Poly-Unsaturated Fatty Acids Composition in Human Milk: A Prospective Cohort Study |
| title_fullStr | The Association between Maternal Oral Inflammation and Neutrophil Phenotypes and Poly-Unsaturated Fatty Acids Composition in Human Milk: A Prospective Cohort Study |
| title_full_unstemmed | The Association between Maternal Oral Inflammation and Neutrophil Phenotypes and Poly-Unsaturated Fatty Acids Composition in Human Milk: A Prospective Cohort Study |
| title_short | The Association between Maternal Oral Inflammation and Neutrophil Phenotypes and Poly-Unsaturated Fatty Acids Composition in Human Milk: A Prospective Cohort Study |
| title_sort | association between maternal oral inflammation and neutrophil phenotypes and poly unsaturated fatty acids composition in human milk a prospective cohort study |
| topic | neutrophils breast milk oral inflammatory load periodontal diseases oral polymorphonuclear neutrophils human milk |
| url | https://www.mdpi.com/2073-4409/11/24/4110 |
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