The Effect of Glucose and Poloxamer 188 on Red-Blood-Cell Aggregation
Glucose metabolism disorders contribute to the development of various diseases. Numerous studies show that these disorders not only change the normal values of biochemical parameters but also affect the mechanical properties of blood. To show the influence of glucose and poloxamer 188 (P188) on the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/12/886 |
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| author | Alicja Szołna-Chodór Bronisław Grzegorzewski |
| author_facet | Alicja Szołna-Chodór Bronisław Grzegorzewski |
| author_sort | Alicja Szołna-Chodór |
| collection | DOAJ |
| description | Glucose metabolism disorders contribute to the development of various diseases. Numerous studies show that these disorders not only change the normal values of biochemical parameters but also affect the mechanical properties of blood. To show the influence of glucose and poloxamer 188 (P188) on the mechanical properties of a red-blood-cell (RBC) suspension, we studied the aggregation of the cells. To show the mechanisms of the mechanical properties of blood, we studied the effects of glucose and poloxamer 188 (P188) on red-blood-cell aggregation. We used a model in which cells were suspended in a dextran 70 solution at a concentration of 2 g/dL with glucose and P188 at concentrations of 0–3 g/dL and 0–3 mg/mL, respectively. RBC aggregation was determined using an aggregometer, and measurements were performed every 4 min for 1 h. Such a procedure enabled the incubation of RBCs in solution. The aggregation index determined from the obtained syllectograms was used as a measure of aggregation. Both the presence of glucose and that of P188 increased the aggregation index with the incubation time until saturation was reached. The time needed for the saturation of the aggregation index increased with increasing glucose and P188 concentrations. As the concentrations of these components increased, the joint effect of glucose and P188 increased the weakening of RBC aggregation. The mechanisms of the observed changes in RBC aggregation in glucose and P188 solutions are discussed. |
| first_indexed | 2024-03-10T03:34:53Z |
| format | Article |
| id | doaj.art-15ba12187a4b4c76a0b73afc1d5fb91d |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T03:34:53Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-15ba12187a4b4c76a0b73afc1d5fb91d2023-11-23T09:32:19ZengMDPI AGMetabolites2218-19892021-12-01111288610.3390/metabo11120886The Effect of Glucose and Poloxamer 188 on Red-Blood-Cell AggregationAlicja Szołna-Chodór0Bronisław Grzegorzewski1Department of Biophysics, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, ul. Jagiellońska 15, 85-067 Bydgoszcz, PolandDepartment of Biophysics, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, ul. Jagiellońska 15, 85-067 Bydgoszcz, PolandGlucose metabolism disorders contribute to the development of various diseases. Numerous studies show that these disorders not only change the normal values of biochemical parameters but also affect the mechanical properties of blood. To show the influence of glucose and poloxamer 188 (P188) on the mechanical properties of a red-blood-cell (RBC) suspension, we studied the aggregation of the cells. To show the mechanisms of the mechanical properties of blood, we studied the effects of glucose and poloxamer 188 (P188) on red-blood-cell aggregation. We used a model in which cells were suspended in a dextran 70 solution at a concentration of 2 g/dL with glucose and P188 at concentrations of 0–3 g/dL and 0–3 mg/mL, respectively. RBC aggregation was determined using an aggregometer, and measurements were performed every 4 min for 1 h. Such a procedure enabled the incubation of RBCs in solution. The aggregation index determined from the obtained syllectograms was used as a measure of aggregation. Both the presence of glucose and that of P188 increased the aggregation index with the incubation time until saturation was reached. The time needed for the saturation of the aggregation index increased with increasing glucose and P188 concentrations. As the concentrations of these components increased, the joint effect of glucose and P188 increased the weakening of RBC aggregation. The mechanisms of the observed changes in RBC aggregation in glucose and P188 solutions are discussed.https://www.mdpi.com/2218-1989/11/12/886RBC aggregationglucosepoloxamer 188 |
| spellingShingle | Alicja Szołna-Chodór Bronisław Grzegorzewski The Effect of Glucose and Poloxamer 188 on Red-Blood-Cell Aggregation Metabolites RBC aggregation glucose poloxamer 188 |
| title | The Effect of Glucose and Poloxamer 188 on Red-Blood-Cell Aggregation |
| title_full | The Effect of Glucose and Poloxamer 188 on Red-Blood-Cell Aggregation |
| title_fullStr | The Effect of Glucose and Poloxamer 188 on Red-Blood-Cell Aggregation |
| title_full_unstemmed | The Effect of Glucose and Poloxamer 188 on Red-Blood-Cell Aggregation |
| title_short | The Effect of Glucose and Poloxamer 188 on Red-Blood-Cell Aggregation |
| title_sort | effect of glucose and poloxamer 188 on red blood cell aggregation |
| topic | RBC aggregation glucose poloxamer 188 |
| url | https://www.mdpi.com/2218-1989/11/12/886 |
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