Traditional Chinese Medicine prescription Huang-Qi-Jian-Zhong-Tang ameliorates indomethacin-induced duodenal ulcers in rats by affecting NF-κB and STAT signaling pathways
Huang-Qi-Jian-Zhong-Tang (HQJZT) is a well-known traditional Chinese herbal formulation. This study aimed to investigate the duodenoprotective properties of HQJZT against Indomethacin (IND)-induced duodenal ulceration in rats, and the mechanisms involved, particularly through NF-κB and STAT signalin...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Biomedicine & Pharmacotherapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332222012550 |
| _version_ | 1811221137061838848 |
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| author | Houpan Song Jingyue Qiu Chang Yu Meng Xiong Chen Ou Baoping Ren Meiqi Zhong Meiyan Zeng Qinghua Peng |
| author_facet | Houpan Song Jingyue Qiu Chang Yu Meng Xiong Chen Ou Baoping Ren Meiqi Zhong Meiyan Zeng Qinghua Peng |
| author_sort | Houpan Song |
| collection | DOAJ |
| description | Huang-Qi-Jian-Zhong-Tang (HQJZT) is a well-known traditional Chinese herbal formulation. This study aimed to investigate the duodenoprotective properties of HQJZT against Indomethacin (IND)-induced duodenal ulceration in rats, and the mechanisms involved, particularly through NF-κB and STAT signaling pathways. Our results showed that HQJZT completely protected the duodenal mucosa from ulceration caused by IND, as indicated by improved macroscopic and histological appearances. There was a significant decrease in ulcer index and microscopic score, an increase in villus height and crypt depth, and a normalization of the tissue architecture of the duodenum in rats following HQJZT treatment. Blood flow into the duodenal mucosa was significantly increased after HQJZT administration. HQJZT significantly increased PGE2 and NO levels in the duodenal mucosa. A significant reduction in the production of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α was observed in the duodenal mucosa under treatment with HQJZT. Mechanistically, the administration of HQJZT significantly lowered the duodenal protein expression of inflammation-related genes, including p-NF-κB and p-IκBβ, compared with the ulcer control group. Furthermore, the STAT signaling pathway-related protein markers p-JAK and p-STAT were significantly reduced in the HQJZT (1.30 and 2.60 g/kg) groups. As a result of these findings, HQJZT alleviates duodenal mucosal ulcers caused by IND. A protective effect of HQJZT on duodenal ulcers is attributed to its ability to improve mucosal blood flow, stimulate the production of cytoprotective mediators, minimize proinflammatory cytokines, and block the activation of NF-κB and STAT signaling pathways. |
| first_indexed | 2024-04-12T07:54:07Z |
| format | Article |
| id | doaj.art-15bab9042b8143fbb0ebd4bd20acb0e2 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-12T07:54:07Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-15bab9042b8143fbb0ebd4bd20acb0e22022-12-22T03:41:32ZengElsevierBiomedicine & Pharmacotherapy0753-33222022-12-01156113866Traditional Chinese Medicine prescription Huang-Qi-Jian-Zhong-Tang ameliorates indomethacin-induced duodenal ulcers in rats by affecting NF-κB and STAT signaling pathwaysHoupan Song0Jingyue Qiu1Chang Yu2Meng Xiong3Chen Ou4Baoping Ren5Meiqi Zhong6Meiyan Zeng7Qinghua Peng8Hunan Provincial Key Laboratory of Diagnostic Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaHunan Provincial Key Laboratory of Diagnostic Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaHunan Provincial Key Laboratory of Diagnostic Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaHunan Provincial Key Laboratory of Diagnostic Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaHunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with TCM, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaHunan Provincial Key Laboratory of Diagnostic Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaHunan Provincial Key Laboratory of Diagnostic Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaCollege of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Correspondence to: College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, 300 Xueshi Road, Yuelu District, Changsha, Hunan 410208, China.Hunan Provincial Key Laboratory of Diagnostic Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China; Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with TCM, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Correspondence to: Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with TCM, Hunan University of Chinese Medicine, 300 Xueshi Road, Yuelu District, Changsha, Hunan 410208, China.Huang-Qi-Jian-Zhong-Tang (HQJZT) is a well-known traditional Chinese herbal formulation. This study aimed to investigate the duodenoprotective properties of HQJZT against Indomethacin (IND)-induced duodenal ulceration in rats, and the mechanisms involved, particularly through NF-κB and STAT signaling pathways. Our results showed that HQJZT completely protected the duodenal mucosa from ulceration caused by IND, as indicated by improved macroscopic and histological appearances. There was a significant decrease in ulcer index and microscopic score, an increase in villus height and crypt depth, and a normalization of the tissue architecture of the duodenum in rats following HQJZT treatment. Blood flow into the duodenal mucosa was significantly increased after HQJZT administration. HQJZT significantly increased PGE2 and NO levels in the duodenal mucosa. A significant reduction in the production of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α was observed in the duodenal mucosa under treatment with HQJZT. Mechanistically, the administration of HQJZT significantly lowered the duodenal protein expression of inflammation-related genes, including p-NF-κB and p-IκBβ, compared with the ulcer control group. Furthermore, the STAT signaling pathway-related protein markers p-JAK and p-STAT were significantly reduced in the HQJZT (1.30 and 2.60 g/kg) groups. As a result of these findings, HQJZT alleviates duodenal mucosal ulcers caused by IND. A protective effect of HQJZT on duodenal ulcers is attributed to its ability to improve mucosal blood flow, stimulate the production of cytoprotective mediators, minimize proinflammatory cytokines, and block the activation of NF-κB and STAT signaling pathways.http://www.sciencedirect.com/science/article/pii/S0753332222012550Traditional Chinese MedicineHQJZTDuodenal ulcerInflammationNF-κBSTAT |
| spellingShingle | Houpan Song Jingyue Qiu Chang Yu Meng Xiong Chen Ou Baoping Ren Meiqi Zhong Meiyan Zeng Qinghua Peng Traditional Chinese Medicine prescription Huang-Qi-Jian-Zhong-Tang ameliorates indomethacin-induced duodenal ulcers in rats by affecting NF-κB and STAT signaling pathways Biomedicine & Pharmacotherapy Traditional Chinese Medicine HQJZT Duodenal ulcer Inflammation NF-κB STAT |
| title | Traditional Chinese Medicine prescription Huang-Qi-Jian-Zhong-Tang ameliorates indomethacin-induced duodenal ulcers in rats by affecting NF-κB and STAT signaling pathways |
| title_full | Traditional Chinese Medicine prescription Huang-Qi-Jian-Zhong-Tang ameliorates indomethacin-induced duodenal ulcers in rats by affecting NF-κB and STAT signaling pathways |
| title_fullStr | Traditional Chinese Medicine prescription Huang-Qi-Jian-Zhong-Tang ameliorates indomethacin-induced duodenal ulcers in rats by affecting NF-κB and STAT signaling pathways |
| title_full_unstemmed | Traditional Chinese Medicine prescription Huang-Qi-Jian-Zhong-Tang ameliorates indomethacin-induced duodenal ulcers in rats by affecting NF-κB and STAT signaling pathways |
| title_short | Traditional Chinese Medicine prescription Huang-Qi-Jian-Zhong-Tang ameliorates indomethacin-induced duodenal ulcers in rats by affecting NF-κB and STAT signaling pathways |
| title_sort | traditional chinese medicine prescription huang qi jian zhong tang ameliorates indomethacin induced duodenal ulcers in rats by affecting nf κb and stat signaling pathways |
| topic | Traditional Chinese Medicine HQJZT Duodenal ulcer Inflammation NF-κB STAT |
| url | http://www.sciencedirect.com/science/article/pii/S0753332222012550 |
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