A Novel Way to Monitor Urine Concentration: Fluorescent Concentration Matrices
Background: The amount of water found in urine is important diagnostic information; nevertheless it is not yet directly determined. Indirectly, the water content in urine is expressed by its density (specific gravity). However, without the diuresis value it is not possible to determine whether t...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2015-01-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://jcdr.net/articles/PDF/5441/8990_CE(Ra)_F(Sh)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
Summary: | Background: The amount of water found in urine is important
diagnostic information; nevertheless it is not yet directly
determined. Indirectly, the water content in urine is expressed by
its density (specific gravity). However, without the diuresis value
it is not possible to determine whether the increase in density
of urine is due to a decrease in water secretion or an increase
in the concentration of secreted substances. This problem can
be solved by the use of fluorescent concentration 3D-matrices
which characterise urine concentration through the pφ (or -logφ)
value of the first fluorescence centre.
Materials and Methods: The urine fluorescent concentration
3D-matrix was created by the alignment of the synchronous
spectra of the dilution series of urine starting from undiluted (pφ
= 0) to 1000-fold diluted urine (pφ = 3).
Results: Using the fluorescence concentration 3D-matrix
analysis of the urine samples from healthy individuals, a reference
range was established for the value pφ, determining the normal,
concentrated or diluted type of urine. The diagnostic potential
of this approach was tested on urine samples from two patients
with a chronic glomerulonephritis.
Conclusion: The pφ value of the urine fluorescence concentration
3D-matrix analysis determines whether the urine sample falls
within the normal, concentrated or diluted type of urine. This
parameter can be directly utilised in sportsmen’s hydration state
monitoring, as well as in the diagnosis and treatment of serious
diseases. An important advantage of this novel diagnostic
approach is that a 12/24 h urine collection is not required, which
predetermines it for use especially within paediatrics. |
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ISSN: | 2249-782X 0973-709X |