The Novel Lipopeptide Poaeamide of the Endophyte Pseudomonas poae RE*1-1-14 Is Involved in Pathogen Suppression and Root Colonization
Endophytic Pseudomonas poae strain RE*1-1-14 was originally isolated from internal root tissue of sugar beet plants and shown to suppress growth of the fungal pathogen Rhizoctonia solani both in vitro and in the field. To identify genes involved in its biocontrol activity, RE*1-1-14 random mutagenes...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2015-07-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-14-0406-R |
| _version_ | 1811291244302696448 |
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| author | Christin Zachow Ghazaleh Jahanshah Irene de Bruijn Chunxu Song Federica Ianni Zoltán Pataj Heike Gerhardt Isabelle Pianet Michael Lämmerhofer Gabriele Berg Harald Gross Jos M. Raaijmakers |
| author_facet | Christin Zachow Ghazaleh Jahanshah Irene de Bruijn Chunxu Song Federica Ianni Zoltán Pataj Heike Gerhardt Isabelle Pianet Michael Lämmerhofer Gabriele Berg Harald Gross Jos M. Raaijmakers |
| author_sort | Christin Zachow |
| collection | DOAJ |
| description | Endophytic Pseudomonas poae strain RE*1-1-14 was originally isolated from internal root tissue of sugar beet plants and shown to suppress growth of the fungal pathogen Rhizoctonia solani both in vitro and in the field. To identify genes involved in its biocontrol activity, RE*1-1-14 random mutagenesis and sequencing led to the identification of a nonribosomal peptide synthetase (NRPS) gene cluster predicted to encode a lipopeptide (LP) with a 10-amino-acid peptide moiety. The two unlinked gene clusters consisted of three NRPS genes, designated poaA (cluster 1) and poaB and poaC (cluster 2), spanning approximately 33.7 kb. In silico analysis followed by chemical analyses revealed that the encoded LP, designated poaeamide, is a structurally new member of the orfamide family. Poaeamide inhibited mycelial growth of R. solani and different oomycetes, including Phytophthora capsici, P. infestans, and Pythium ultimum. The novel LP was shown to be essential for swarming motility of strain RE*1-1-14 and had an impact on root colonization of sugar beet seedlings The poaeamide-deficient mutant colonized the rhizosphere and upper plant cortex at higher densities and with more scattered colonization patterns than the wild type. Collectively, these results indicate that Pseudomonas poae RE*1-1-14 produces a structurally new LP that is relevant for its antagonistic activity against soilborne plant pathogens and for colonization of sugar beet roots. |
| first_indexed | 2024-04-13T04:26:27Z |
| format | Article |
| id | doaj.art-15bf22afa14546fdb93db5b5ec5116c4 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:26:27Z |
| publishDate | 2015-07-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-15bf22afa14546fdb93db5b5ec5116c42022-12-22T03:02:30ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062015-07-0128780081010.1094/MPMI-12-14-0406-RThe Novel Lipopeptide Poaeamide of the Endophyte Pseudomonas poae RE*1-1-14 Is Involved in Pathogen Suppression and Root ColonizationChristin Zachow0Ghazaleh Jahanshah1Irene de Bruijn2Chunxu Song3Federica Ianni4Zoltán Pataj5Heike Gerhardt6Isabelle Pianet7Michael Lämmerhofer8Gabriele Berg9Harald Gross10Jos M. Raaijmakers11Austrian Centre of Industrial Biotechnology (ACIB GmbH), 8010 Graz, Austria;Pharmaceutical Institute, Department of Pharmaceutical Biology, University of Tübingen, 72076 Tübingen, Germany;Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands;Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands;Pharmaceutical Institute, Department of Pharmaceutical Analysis and Bioanalysis, University of Tübingen;Pharmaceutical Institute, Department of Pharmaceutical Analysis and Bioanalysis, University of Tübingen;Pharmaceutical Institute, Department of Pharmaceutical Analysis and Bioanalysis, University of Tübingen;CESAMO-ISM, UMR 5255, CNRS, Université Bordeaux I, 351 Cours de la Libération, F-33405 Talence, France;Pharmaceutical Institute, Department of Pharmaceutical Analysis and Bioanalysis, University of Tübingen;Institute of Environmental Biotechnology, Graz University of Technology, 8010 Graz, AustriaPharmaceutical Institute, Department of Pharmaceutical Biology, University of Tübingen, 72076 Tübingen, Germany;Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands;Endophytic Pseudomonas poae strain RE*1-1-14 was originally isolated from internal root tissue of sugar beet plants and shown to suppress growth of the fungal pathogen Rhizoctonia solani both in vitro and in the field. To identify genes involved in its biocontrol activity, RE*1-1-14 random mutagenesis and sequencing led to the identification of a nonribosomal peptide synthetase (NRPS) gene cluster predicted to encode a lipopeptide (LP) with a 10-amino-acid peptide moiety. The two unlinked gene clusters consisted of three NRPS genes, designated poaA (cluster 1) and poaB and poaC (cluster 2), spanning approximately 33.7 kb. In silico analysis followed by chemical analyses revealed that the encoded LP, designated poaeamide, is a structurally new member of the orfamide family. Poaeamide inhibited mycelial growth of R. solani and different oomycetes, including Phytophthora capsici, P. infestans, and Pythium ultimum. The novel LP was shown to be essential for swarming motility of strain RE*1-1-14 and had an impact on root colonization of sugar beet seedlings The poaeamide-deficient mutant colonized the rhizosphere and upper plant cortex at higher densities and with more scattered colonization patterns than the wild type. Collectively, these results indicate that Pseudomonas poae RE*1-1-14 produces a structurally new LP that is relevant for its antagonistic activity against soilborne plant pathogens and for colonization of sugar beet roots.https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-14-0406-R |
| spellingShingle | Christin Zachow Ghazaleh Jahanshah Irene de Bruijn Chunxu Song Federica Ianni Zoltán Pataj Heike Gerhardt Isabelle Pianet Michael Lämmerhofer Gabriele Berg Harald Gross Jos M. Raaijmakers The Novel Lipopeptide Poaeamide of the Endophyte Pseudomonas poae RE*1-1-14 Is Involved in Pathogen Suppression and Root Colonization Molecular Plant-Microbe Interactions |
| title | The Novel Lipopeptide Poaeamide of the Endophyte Pseudomonas poae RE*1-1-14 Is Involved in Pathogen Suppression and Root Colonization |
| title_full | The Novel Lipopeptide Poaeamide of the Endophyte Pseudomonas poae RE*1-1-14 Is Involved in Pathogen Suppression and Root Colonization |
| title_fullStr | The Novel Lipopeptide Poaeamide of the Endophyte Pseudomonas poae RE*1-1-14 Is Involved in Pathogen Suppression and Root Colonization |
| title_full_unstemmed | The Novel Lipopeptide Poaeamide of the Endophyte Pseudomonas poae RE*1-1-14 Is Involved in Pathogen Suppression and Root Colonization |
| title_short | The Novel Lipopeptide Poaeamide of the Endophyte Pseudomonas poae RE*1-1-14 Is Involved in Pathogen Suppression and Root Colonization |
| title_sort | novel lipopeptide poaeamide of the endophyte pseudomonas poae re 1 1 14 is involved in pathogen suppression and root colonization |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-14-0406-R |
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