The Microbiome and Antibiotic Resistome in Soil under Biodegradable Composite Carbon Source Amendment
The decomposition of biodegradable composite carbon sources generates a large amount of biodegradable microplastics, which may not only furnish microbial denitrification, but might also pose potential environmental risks. In the present study, the effects of different dosages of a biodegradable comp...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Journal of Xenobiotics |
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| Online Access: | https://www.mdpi.com/2039-4713/13/3/27 |
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| author | Zhongchen Yang Yanhong Lou Xianghui Yan Hong Pan Hui Wang Quangang Yang Yajie Sun Yuping Zhuge |
| author_facet | Zhongchen Yang Yanhong Lou Xianghui Yan Hong Pan Hui Wang Quangang Yang Yajie Sun Yuping Zhuge |
| author_sort | Zhongchen Yang |
| collection | DOAJ |
| description | The decomposition of biodegradable composite carbon sources generates a large amount of biodegradable microplastics, which may not only furnish microbial denitrification, but might also pose potential environmental risks. In the present study, the effects of different dosages of a biodegradable composite carbon source on the microbial communities, the nitrogen metabolic pathways and the antibiotic resistome were explored through Illumina MiSeq sequencing analysis and metagenomic analysis. The results of partial least-square discriminant analysis (PLS-DA) and analysis of similarity (ANOSIM) demonstrated that the response of the bacterial community to a biodegradable composite carbon source was more obvious than the fungal community. The application of biodegradable microplastics diminished the complexity of the microbial communities to some extent and obviously stimulated denitrification. Antibiotics resistance gene (ARG) dispersal was not evidently accelerated after the addition of biodegradable composite carbon source. <i>Lysobacter</i>, <i>Methylobacillus</i>, <i>Phyllobacterium</i>, <i>Sinorhizobium</i>, <i>Sphingomonas</i> from <i>Proteobacteria</i> and <i>Actinomadura</i>, <i>Agromyces</i>, <i>Gaiella</i> and <i>Micromonospora</i> from <i>Actinobacteria</i> were the major ARG hosts. Overall, the addition of a biodegradable composite carbon source shaped microbial communities and their antibiotic resistance profiles in this study. |
| first_indexed | 2024-03-10T22:33:42Z |
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| institution | Directory Open Access Journal |
| issn | 2039-4705 2039-4713 |
| language | English |
| last_indexed | 2024-03-10T22:33:42Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
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| series | Journal of Xenobiotics |
| spelling | doaj.art-15c4e1bd170b4b699ad774aeb2eed9da2023-11-19T11:30:00ZengMDPI AGJournal of Xenobiotics2039-47052039-47132023-08-0113342443810.3390/jox13030027The Microbiome and Antibiotic Resistome in Soil under Biodegradable Composite Carbon Source AmendmentZhongchen Yang0Yanhong Lou1Xianghui Yan2Hong Pan3Hui Wang4Quangang Yang5Yajie Sun6Yuping Zhuge7National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai’an 271018, ChinaNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai’an 271018, ChinaNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai’an 271018, ChinaNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai’an 271018, ChinaNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai’an 271018, ChinaNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai’an 271018, ChinaNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai’an 271018, ChinaNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai’an 271018, ChinaThe decomposition of biodegradable composite carbon sources generates a large amount of biodegradable microplastics, which may not only furnish microbial denitrification, but might also pose potential environmental risks. In the present study, the effects of different dosages of a biodegradable composite carbon source on the microbial communities, the nitrogen metabolic pathways and the antibiotic resistome were explored through Illumina MiSeq sequencing analysis and metagenomic analysis. The results of partial least-square discriminant analysis (PLS-DA) and analysis of similarity (ANOSIM) demonstrated that the response of the bacterial community to a biodegradable composite carbon source was more obvious than the fungal community. The application of biodegradable microplastics diminished the complexity of the microbial communities to some extent and obviously stimulated denitrification. Antibiotics resistance gene (ARG) dispersal was not evidently accelerated after the addition of biodegradable composite carbon source. <i>Lysobacter</i>, <i>Methylobacillus</i>, <i>Phyllobacterium</i>, <i>Sinorhizobium</i>, <i>Sphingomonas</i> from <i>Proteobacteria</i> and <i>Actinomadura</i>, <i>Agromyces</i>, <i>Gaiella</i> and <i>Micromonospora</i> from <i>Actinobacteria</i> were the major ARG hosts. Overall, the addition of a biodegradable composite carbon source shaped microbial communities and their antibiotic resistance profiles in this study.https://www.mdpi.com/2039-4713/13/3/27biodegradable microplasticscomposite carbon sourceantibiotic resistance genesmetagenomic sequencingmicrobial community |
| spellingShingle | Zhongchen Yang Yanhong Lou Xianghui Yan Hong Pan Hui Wang Quangang Yang Yajie Sun Yuping Zhuge The Microbiome and Antibiotic Resistome in Soil under Biodegradable Composite Carbon Source Amendment Journal of Xenobiotics biodegradable microplastics composite carbon source antibiotic resistance genes metagenomic sequencing microbial community |
| title | The Microbiome and Antibiotic Resistome in Soil under Biodegradable Composite Carbon Source Amendment |
| title_full | The Microbiome and Antibiotic Resistome in Soil under Biodegradable Composite Carbon Source Amendment |
| title_fullStr | The Microbiome and Antibiotic Resistome in Soil under Biodegradable Composite Carbon Source Amendment |
| title_full_unstemmed | The Microbiome and Antibiotic Resistome in Soil under Biodegradable Composite Carbon Source Amendment |
| title_short | The Microbiome and Antibiotic Resistome in Soil under Biodegradable Composite Carbon Source Amendment |
| title_sort | microbiome and antibiotic resistome in soil under biodegradable composite carbon source amendment |
| topic | biodegradable microplastics composite carbon source antibiotic resistance genes metagenomic sequencing microbial community |
| url | https://www.mdpi.com/2039-4713/13/3/27 |
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