DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia
Pollen taxon in sediment samples can be identified by analyzing pollen morphology. Identification of related species based on pollen morphology is difficult and is limited primarily to genus or family. Because pollen grains of various ages are preserved at below 0 °C in glaciers and thus are more li...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2013-01-01
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| Series: | Environmental Research Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/1748-9326/8/1/014032 |
| _version_ | 1797748404593360896 |
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| author | Fumio Nakazawa Jun Uetake Yoshihisa Suyama Ryo Kaneko Nozomu Takeuchi Koji Fujita Hideaki Motoyama Satoshi Imura Hiroshi Kanda |
| author_facet | Fumio Nakazawa Jun Uetake Yoshihisa Suyama Ryo Kaneko Nozomu Takeuchi Koji Fujita Hideaki Motoyama Satoshi Imura Hiroshi Kanda |
| author_sort | Fumio Nakazawa |
| collection | DOAJ |
| description | Pollen taxon in sediment samples can be identified by analyzing pollen morphology. Identification of related species based on pollen morphology is difficult and is limited primarily to genus or family. Because pollen grains of various ages are preserved at below 0 °C in glaciers and thus are more likely to remain intact or to suffer little DNA fragmentation, genetic information from such pollen grains should enable identification of plant taxa below the genus level. However, no published studies have attempted detailed identification using DNA sequences obtained from pollen found in glaciers. As a preliminary step, this study attempted to analyze the DNA of Pinus pollen grains extracted from surface snow collected from the Belukha glacier in the Altai Mountains of Russia in the summer of 2003. A 150-bp rpoB fragment from the chloroplast genome in each Pinus pollen grain was amplified by polymerase chain reaction, and DNA products were sequenced to identify them at the section level. A total of 105 pollen grains were used for the test, and sequences were obtained from eight grains. From the sequences obtained, the pollen grains were identified as belonging to the section Quinquefoliae . Trees of the extant species Pinus sibirica in the section Quinquefoliae are currently found surrounding the glacier. The consistency of results for this section suggests that the pollen in the glacier originated from the same Pinus trees as those found in the immediate surroundings. |
| first_indexed | 2024-03-12T16:05:14Z |
| format | Article |
| id | doaj.art-15c746e759e748fd829338fe96038b6a |
| institution | Directory Open Access Journal |
| issn | 1748-9326 |
| language | English |
| last_indexed | 2024-03-12T16:05:14Z |
| publishDate | 2013-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Environmental Research Letters |
| spelling | doaj.art-15c746e759e748fd829338fe96038b6a2023-08-09T14:23:45ZengIOP PublishingEnvironmental Research Letters1748-93262013-01-018101403210.1088/1748-9326/8/1/014032DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, RussiaFumio Nakazawa0Jun Uetake1Yoshihisa Suyama2Ryo Kaneko3Nozomu Takeuchi4Koji Fujita5Hideaki Motoyama6Satoshi Imura7Hiroshi Kanda8National Institute of Polar Research , 10-3 Midori-cho, Tachikawa, Tokyo 190-8518, Japan; Transdisciplinary Research Integration Center , 2nd Floor, Kamiyacho Central Place Building, 4-3-13 Toranomon, Minato-ku, Tokyo 105-0001, JapanNational Institute of Polar Research , 10-3 Midori-cho, Tachikawa, Tokyo 190-8518, Japan; Transdisciplinary Research Integration Center , 2nd Floor, Kamiyacho Central Place Building, 4-3-13 Toranomon, Minato-ku, Tokyo 105-0001, JapanField Science Center, Graduate School of Agricultural Science, Tohoku University , 232-3 Yomogida, Naruko-onsen, Osaki, Miyagi 989-6711, JapanAtmosphere and Ocean Research Institute, The University of Tokyo , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, JapanGraduate School of Science, Chiba University , 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, JapanGraduate School of Environmental Studies, Nagoya University , F3-1(200) Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, JapanNational Institute of Polar Research , 10-3 Midori-cho, Tachikawa, Tokyo 190-8518, JapanNational Institute of Polar Research , 10-3 Midori-cho, Tachikawa, Tokyo 190-8518, JapanNational Institute of Polar Research , 10-3 Midori-cho, Tachikawa, Tokyo 190-8518, JapanPollen taxon in sediment samples can be identified by analyzing pollen morphology. Identification of related species based on pollen morphology is difficult and is limited primarily to genus or family. Because pollen grains of various ages are preserved at below 0 °C in glaciers and thus are more likely to remain intact or to suffer little DNA fragmentation, genetic information from such pollen grains should enable identification of plant taxa below the genus level. However, no published studies have attempted detailed identification using DNA sequences obtained from pollen found in glaciers. As a preliminary step, this study attempted to analyze the DNA of Pinus pollen grains extracted from surface snow collected from the Belukha glacier in the Altai Mountains of Russia in the summer of 2003. A 150-bp rpoB fragment from the chloroplast genome in each Pinus pollen grain was amplified by polymerase chain reaction, and DNA products were sequenced to identify them at the section level. A total of 105 pollen grains were used for the test, and sequences were obtained from eight grains. From the sequences obtained, the pollen grains were identified as belonging to the section Quinquefoliae . Trees of the extant species Pinus sibirica in the section Quinquefoliae are currently found surrounding the glacier. The consistency of results for this section suggests that the pollen in the glacier originated from the same Pinus trees as those found in the immediate surroundings.https://doi.org/10.1088/1748-9326/8/1/014032pollenDNAglacierPinusAltai |
| spellingShingle | Fumio Nakazawa Jun Uetake Yoshihisa Suyama Ryo Kaneko Nozomu Takeuchi Koji Fujita Hideaki Motoyama Satoshi Imura Hiroshi Kanda DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia Environmental Research Letters pollen DNA glacier Pinus Altai |
| title | DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia |
| title_full | DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia |
| title_fullStr | DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia |
| title_full_unstemmed | DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia |
| title_short | DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia |
| title_sort | dna analysis for section identification of individual pinus pollen grains from belukha glacier altai mountains russia |
| topic | pollen DNA glacier Pinus Altai |
| url | https://doi.org/10.1088/1748-9326/8/1/014032 |
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