Urinary N-acetyltyramine-O,β-glucuronide in Persons with Onchocerciasis<i>-</i>Associated Epilepsy
We investigated urinary N-acetyltyramine-O,β-glucuronide (NATOG) levels as a biomarker for active <i>Onchocerca volvulus</i> infection in an onchocerciasis-endemic area in the Democratic Republic of Congo with a high epilepsy prevalence. Urinary NATOG was measured in non-epilept...
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2020-03-01
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author | An Hotterbeekx Alfred Dusabimana Michel Mandro Germain M Abhafule Wonya’Rossy Deogratias Joseph N. Siewe Fodjo Steven Abrams Robert Colebunders |
author_facet | An Hotterbeekx Alfred Dusabimana Michel Mandro Germain M Abhafule Wonya’Rossy Deogratias Joseph N. Siewe Fodjo Steven Abrams Robert Colebunders |
author_sort | An Hotterbeekx |
collection | DOAJ |
description | We investigated urinary N-acetyltyramine-O,β-glucuronide (NATOG) levels as a biomarker for active <i>Onchocerca volvulus</i> infection in an onchocerciasis-endemic area in the Democratic Republic of Congo with a high epilepsy prevalence. Urinary NATOG was measured in non-epileptic men with and without <i>O. volvulus</i> infection, and in <i>O. volvulus-</i>infected<i> </i>persons with epilepsy (PWE). Urinary NATOG concentration was positively associated with microfilarial density (<i>p </i>< 0.001). The median urinary NATOG concentration was higher in PWE (3.67 µM) compared to men without epilepsy (1.74 µM), <i>p </i>= 0.017; and was higher in persons with severe (7.62 µM) compared to mild epilepsy (2.16 µM); <i>p </i>= 0.008. Non-epileptic participants with and without <i>O. volvulus </i>infection had similar NATOG levels (2.23 µM and 0.71 µM, <i>p</i> = 0.426). In a receiver operating characteristic curve analysis to investigate the diagnostic value of urinary NATOG, the area under the curve was 0.721 (95% CI: 0.633−0.797). Using the previously proposed cut-off value of 13 µM to distinguish between an active <i>O. volvulus </i>infection and an uninfected state, the sensitivity was 15.9% and the specificity 95.9%. In conclusion, an <i>O. volvulus</i> infection is associated with an increased urinary NATOG concentration, which correlates with the individual parasitic load. However, the NATOG concentration has a low discriminating power to differentiate between infected and uninfected individuals. |
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last_indexed | 2024-04-11T12:53:36Z |
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spelling | doaj.art-15cdedbb0a62446fa1797ec14ecf37442022-12-22T04:23:08ZengMDPI AGPathogens2076-08172020-03-019319110.3390/pathogens9030191pathogens9030191Urinary N-acetyltyramine-O,β-glucuronide in Persons with Onchocerciasis<i>-</i>Associated EpilepsyAn Hotterbeekx0Alfred Dusabimana1Michel Mandro2Germain M Abhafule3Wonya’Rossy Deogratias4Joseph N. Siewe Fodjo5Steven Abrams6Robert Colebunders7Global Health Institute, University of Antwerp, B-2610 Wilrijk, BelgiumGlobal Health Institute, University of Antwerp, B-2610 Wilrijk, BelgiumProvincial Health Division Ituri, Ministry of Health, 185 DRC 57 Ituri, CongoProvincial Health Division Ituri, Ministry of Health, 185 DRC 57 Ituri, CongoProvincial Health Division Ituri, Ministry of Health, 185 DRC 57 Ituri, CongoGlobal Health Institute, University of Antwerp, B-2610 Wilrijk, BelgiumGlobal Health Institute, University of Antwerp, B-2610 Wilrijk, BelgiumGlobal Health Institute, University of Antwerp, B-2610 Wilrijk, BelgiumWe investigated urinary N-acetyltyramine-O,β-glucuronide (NATOG) levels as a biomarker for active <i>Onchocerca volvulus</i> infection in an onchocerciasis-endemic area in the Democratic Republic of Congo with a high epilepsy prevalence. Urinary NATOG was measured in non-epileptic men with and without <i>O. volvulus</i> infection, and in <i>O. volvulus-</i>infected<i> </i>persons with epilepsy (PWE). Urinary NATOG concentration was positively associated with microfilarial density (<i>p </i>< 0.001). The median urinary NATOG concentration was higher in PWE (3.67 µM) compared to men without epilepsy (1.74 µM), <i>p </i>= 0.017; and was higher in persons with severe (7.62 µM) compared to mild epilepsy (2.16 µM); <i>p </i>= 0.008. Non-epileptic participants with and without <i>O. volvulus </i>infection had similar NATOG levels (2.23 µM and 0.71 µM, <i>p</i> = 0.426). In a receiver operating characteristic curve analysis to investigate the diagnostic value of urinary NATOG, the area under the curve was 0.721 (95% CI: 0.633−0.797). Using the previously proposed cut-off value of 13 µM to distinguish between an active <i>O. volvulus </i>infection and an uninfected state, the sensitivity was 15.9% and the specificity 95.9%. In conclusion, an <i>O. volvulus</i> infection is associated with an increased urinary NATOG concentration, which correlates with the individual parasitic load. However, the NATOG concentration has a low discriminating power to differentiate between infected and uninfected individuals.https://www.mdpi.com/2076-0817/9/3/191<i>onchocerca volvulus</i>n-acetyltyramine-o,β-glucuronide (natog), epilepsyafricabiomarkerurine |
spellingShingle | An Hotterbeekx Alfred Dusabimana Michel Mandro Germain M Abhafule Wonya’Rossy Deogratias Joseph N. Siewe Fodjo Steven Abrams Robert Colebunders Urinary N-acetyltyramine-O,β-glucuronide in Persons with Onchocerciasis<i>-</i>Associated Epilepsy Pathogens <i>onchocerca volvulus</i> n-acetyltyramine-o,β-glucuronide (natog), epilepsy africa biomarker urine |
title | Urinary N-acetyltyramine-O,β-glucuronide in Persons with Onchocerciasis<i>-</i>Associated Epilepsy |
title_full | Urinary N-acetyltyramine-O,β-glucuronide in Persons with Onchocerciasis<i>-</i>Associated Epilepsy |
title_fullStr | Urinary N-acetyltyramine-O,β-glucuronide in Persons with Onchocerciasis<i>-</i>Associated Epilepsy |
title_full_unstemmed | Urinary N-acetyltyramine-O,β-glucuronide in Persons with Onchocerciasis<i>-</i>Associated Epilepsy |
title_short | Urinary N-acetyltyramine-O,β-glucuronide in Persons with Onchocerciasis<i>-</i>Associated Epilepsy |
title_sort | urinary n acetyltyramine o β glucuronide in persons with onchocerciasis i i associated epilepsy |
topic | <i>onchocerca volvulus</i> n-acetyltyramine-o,β-glucuronide (natog), epilepsy africa biomarker urine |
url | https://www.mdpi.com/2076-0817/9/3/191 |
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