A Saliva-Based RNA Extraction-Free Workflow Integrated With Cas13a for SARS-CoV-2 Detection

A Saliva-Based RNA Extraction-Free Workflow Integrated With Cas13a for SARS-CoV-2 Detection

A major bottleneck in scaling-up COVID-19 testing is the need for sophisticated instruments and well-trained healthcare professionals, which are already overwhelmed due to the pandemic. Moreover, the high-sensitive SARS-CoV-2 diagnostics are contingent on an RNA extraction step, which, in turn, is r...

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Bibliographic Details
Main Authors: Iqbal Azmi, Md Imam Faizan, Rohit Kumar, Siddharth Raj Yadav, Nisha Chaudhary, Deepak Kumar Singh, Ruchika Butola, Aryan Ganotra, Gopal Datt Joshi, Gagan Deep Jhingan, Jawed Iqbal, Mohan C. Joshi, Tanveer Ahmad
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
COVID-19
Crispr-Cas13a
saliva
SARS-CoV-2
CRISPR Diagnostics
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2021.632646/full

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