A Novel Design of a Non-Enzymatic Biosensor for the Detection of Hydrogen Peroxide Based on MWCNT/Co3O4/Hemoglobin Structures (Original Article)
Background: Determining the concentration of hydrogen peroxide in liquids and biological samples is very important because of its effects on human health. This study aimed to design a new electrochemical biosensor based on hemoglobin to detect hydrogen peroxide in serum samples. Methods: In this stu...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Ardabil University of Medical Sciences
2023-10-01
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| Series: | Journal of Ardabil University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jarums.arums.ac.ir/article-1-2327-en.pdf |
| Summary: | Background: Determining the concentration of hydrogen peroxide in liquids and biological samples is very important because of its effects on human health. This study aimed to design a new electrochemical biosensor based on hemoglobin to detect hydrogen peroxide in serum samples.
Methods: In this study, a basic science, a biosensor based on modifying the glassy carbon electrode surface with a nanocomposite consisting of cobalt oxide nanoparticles and multi-walled carbon nanotube functionalized with a carboxyl group (MWCNT/Co3O4) and hemoglobin stabilized on this nanocomposite was made as a biological recognition element.
Results: In optimal conditions, the biosensor was used to measure different concentrations of hydrogen peroxide. The designed biosensor showed a wide linear response range from 10 μM to 500 μM, a detection limit of 0.512 μM, and high reproducibility and stability.
Conclusion: In this innovative research work, MWCNTs/Co3O4 nanocomposite was used to make a diagnostic biosensor. The presented biosensor showed an acceptable performance in the measurement of hydrogen peroxide in serum samples and laboratory solutions. |
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| ISSN: | 2228-7280 2228-7299 |