Ghrelin in the lateral parabrachial nucleus influences the excitability of glucosensing neurons, increases food intake and body weight
Ghrelin plays a pivotal role in the regulation of food intake, body weight and energy metabolism. However, these effects of ghrelin in the lateral par abrachial nucleus (LPBN) are unexplored. C57BL/6J mice and GHSR−/− mice were implanted with cannula above the right LPBN and ghrelin was microinjecte...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bioscientifica
2020-12-01
|
| Series: | Endocrine Connections |
| Subjects: | |
| Online Access: | https://ec.bioscientifica.com/view/journals/ec/9/12/EC-20-0285.xml |
| Summary: | Ghrelin plays a pivotal role in the regulation of food intake, body weight and energy metabolism. However, these effects of ghrelin in the lateral par abrachial nucleus (LPBN) are unexplored. C57BL/6J mice and GHSR−/− mice were implanted with cannula above the right LPBN and ghrelin was microinjected via the cannula to inv estigate effect of ghrelin in the LPBN. In vivo electrophysiological technique was used to record LPBN glucose - sensitive neurons to explore potential udnderlying mechanisms. Microinjection of ghrelin in LPBN significantly increased food intake in the first 3 h, whi le such effect was blocked by [D-Lys3]-GHRP-6 and abolished in GHSR−/− mice. LPBN ghrelin microinjection also significantly increased the firing rate of glucose-excited (GE) n eurons and decreased the firing rate of glucose-inhibited (GI) neurons. Additionally, LPB N ghrelin microinjection also significantly increased c-fos expression. Chronic ghrelin a dministration in the LPBN resulted in significantly increased body weight gain. Meanwhile, no significant changes were observed in both mRNA and protein expression levels of UCP -1 in BAT. These results demonstrated that microinjection of ghrelin in LPBN could incre ase food intake through the interaction with growth hormone secretagogue receptor (GHSR ) in C57BL/6J mice, and its chronic administration could also increase body weight gain . These effects might be associated with altered firing rate in the GE and GI neurons. |
|---|---|
| ISSN: | 2049-3614 2049-3614 |