Monocyte subsets and monocyte-related chemokines in Takayasu arteritis
Abstract The pathogenesis of Takayasu arteritis (TAK) is poorly understood and no previous studies have analyzed monocytes in TAK. This study evaluated monocyte subsets and monocyte-related chemokines in the peripheral blood of TAK patients and healthy controls (HC). Monocyte subsets were identified...
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| Format: | Article |
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Nature Portfolio
2023-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-29369-3 |
| _version_ | 1811165908323794944 |
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| author | Mariana Freitas de Aguiar Heron Torquato Bruno Ramos Salu Ana Cecília Diniz Oliveira Maria Luiza Vilela Oliva Edgar Julian Paredes-Gamero Wayel H. Abdulahad Elisabeth Brouwer Alexandre W. S. de Souza |
| author_facet | Mariana Freitas de Aguiar Heron Torquato Bruno Ramos Salu Ana Cecília Diniz Oliveira Maria Luiza Vilela Oliva Edgar Julian Paredes-Gamero Wayel H. Abdulahad Elisabeth Brouwer Alexandre W. S. de Souza |
| author_sort | Mariana Freitas de Aguiar |
| collection | DOAJ |
| description | Abstract The pathogenesis of Takayasu arteritis (TAK) is poorly understood and no previous studies have analyzed monocytes in TAK. This study evaluated monocyte subsets and monocyte-related chemokines in the peripheral blood of TAK patients and healthy controls (HC). Monocyte subsets were identified as classical (CD14+CD16−), intermediate (CD14+CD16dim), and non-classical (CD14dimCD16high) in the peripheral blood. The chemokines CCL (C–C chemokine ligand)2, CCL3, CCL4, CCL5, CCL7, CXCL (C-X-C motif ligand)10, and CX3CL (C-X3-C motif ligand)1 were measured in the sera. Thirty-two TAK patients and 30 HC were evaluated. Intermediate monocytes were higher in TAK than HC [25.0 cells ×106/L (16.7–52.0) vs. 17.2 cells ×106/L (9.2–25.3); p = 0.014]. Active disease was associated with monocytosis (p = 0.004), increased classical (p = 0.003), and intermediate (p < 0.001) subsets than HC. Prednisone reduced the percentage of non-classical monocytes (p = 0.011). TAK patients had lower CCL3 (p = 0.033) and CCL4 (p = 0.023) levels than HC, whereas CCL22 levels were higher in active TAK compared to the remission state (p = 0.008). Glucocorticoids were associated with lower CXCL10 levels (p = 0.012). In TAK, CCL4 correlated with total (Rho = 0.489; p = 0.005), classical and intermediate monocytes (Rho = 0.448; p = 0.010 and Rho = 0.412; p = 0.019). In conclusion, TAK is associated with altered counts of monocyte subsets in the peripheral blood compared to HC and CCL22 is the chemokine with the strongest association with active disease in TAK. |
| first_indexed | 2024-04-10T15:43:50Z |
| format | Article |
| id | doaj.art-15d0c5aa77924f858e4de0294f0a833a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T15:43:50Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-15d0c5aa77924f858e4de0294f0a833a2023-02-12T12:11:27ZengNature PortfolioScientific Reports2045-23222023-02-0113111210.1038/s41598-023-29369-3Monocyte subsets and monocyte-related chemokines in Takayasu arteritisMariana Freitas de Aguiar0Heron Torquato1Bruno Ramos Salu2Ana Cecília Diniz Oliveira3Maria Luiza Vilela Oliva4Edgar Julian Paredes-Gamero5Wayel H. Abdulahad6Elisabeth Brouwer7Alexandre W. S. de Souza8Rheumatology Division, Department of Medicine, Universidade Federal de São Paulo–Escola Paulista de MedicinaFaculdade de Farmácia, Centro Universitário Braz CubasDepartment of Biochemistry, Universidade Federal de São Paulo–Escola Paulista de MedicinaRheumatology Division, Department of Medicine, Universidade Federal de São Paulo–Escola Paulista de MedicinaDepartment of Biochemistry, Universidade Federal de São Paulo–Escola Paulista de MedicinaFaculdade de Farmácia, Tecnologia de Alimentos e Nutrição, Universidade Federal de Mato Grosso do SulDepartment of Rheumatology and Clinical Immunology, University of Groningen, University Medical Center GroningenDepartment of Rheumatology and Clinical Immunology, University of Groningen, University Medical Center GroningenRheumatology Division, Department of Medicine, Universidade Federal de São Paulo–Escola Paulista de MedicinaAbstract The pathogenesis of Takayasu arteritis (TAK) is poorly understood and no previous studies have analyzed monocytes in TAK. This study evaluated monocyte subsets and monocyte-related chemokines in the peripheral blood of TAK patients and healthy controls (HC). Monocyte subsets were identified as classical (CD14+CD16−), intermediate (CD14+CD16dim), and non-classical (CD14dimCD16high) in the peripheral blood. The chemokines CCL (C–C chemokine ligand)2, CCL3, CCL4, CCL5, CCL7, CXCL (C-X-C motif ligand)10, and CX3CL (C-X3-C motif ligand)1 were measured in the sera. Thirty-two TAK patients and 30 HC were evaluated. Intermediate monocytes were higher in TAK than HC [25.0 cells ×106/L (16.7–52.0) vs. 17.2 cells ×106/L (9.2–25.3); p = 0.014]. Active disease was associated with monocytosis (p = 0.004), increased classical (p = 0.003), and intermediate (p < 0.001) subsets than HC. Prednisone reduced the percentage of non-classical monocytes (p = 0.011). TAK patients had lower CCL3 (p = 0.033) and CCL4 (p = 0.023) levels than HC, whereas CCL22 levels were higher in active TAK compared to the remission state (p = 0.008). Glucocorticoids were associated with lower CXCL10 levels (p = 0.012). In TAK, CCL4 correlated with total (Rho = 0.489; p = 0.005), classical and intermediate monocytes (Rho = 0.448; p = 0.010 and Rho = 0.412; p = 0.019). In conclusion, TAK is associated with altered counts of monocyte subsets in the peripheral blood compared to HC and CCL22 is the chemokine with the strongest association with active disease in TAK.https://doi.org/10.1038/s41598-023-29369-3 |
| spellingShingle | Mariana Freitas de Aguiar Heron Torquato Bruno Ramos Salu Ana Cecília Diniz Oliveira Maria Luiza Vilela Oliva Edgar Julian Paredes-Gamero Wayel H. Abdulahad Elisabeth Brouwer Alexandre W. S. de Souza Monocyte subsets and monocyte-related chemokines in Takayasu arteritis Scientific Reports |
| title | Monocyte subsets and monocyte-related chemokines in Takayasu arteritis |
| title_full | Monocyte subsets and monocyte-related chemokines in Takayasu arteritis |
| title_fullStr | Monocyte subsets and monocyte-related chemokines in Takayasu arteritis |
| title_full_unstemmed | Monocyte subsets and monocyte-related chemokines in Takayasu arteritis |
| title_short | Monocyte subsets and monocyte-related chemokines in Takayasu arteritis |
| title_sort | monocyte subsets and monocyte related chemokines in takayasu arteritis |
| url | https://doi.org/10.1038/s41598-023-29369-3 |
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