Estimation of macrophage inflammatory protein-1α (MIP-1 α) levels in serum and gingival crevicular fluid in periodontal health, disease, and after treatment—A clinico-biochemical study
Objectives: Previous studies have revealed high macrophage inflammatory protein-1α (MIP-1α) levels in the gingival crevicular fluid (GCF) and the serum of patients with chronic periodontitis. However, reports correlating the GCF and serum MIP-1α levels are inadequate. Therefore, the present study es...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Journal of Dr. NTR University of Health Sciences |
| Subjects: | |
| Online Access: | http://www.jdrntruhs.org/article.asp?issn=2277-8632;year=2019;volume=8;issue=2;spage=107;epage=113;aulast=Subramanyam |
| _version_ | 1818150817087094784 |
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| author | Madhu Babu Dandu Subramanyam Sravani Reddy Cheppali Deepa Anumla Bindu Sighinam E Prasuna Ravindra Nagireddy Reddy |
| author_facet | Madhu Babu Dandu Subramanyam Sravani Reddy Cheppali Deepa Anumla Bindu Sighinam E Prasuna Ravindra Nagireddy Reddy |
| author_sort | Madhu Babu Dandu Subramanyam |
| collection | DOAJ |
| description | Objectives: Previous studies have revealed high macrophage inflammatory protein-1α (MIP-1α) levels in the gingival crevicular fluid (GCF) and the serum of patients with chronic periodontitis. However, reports correlating the GCF and serum MIP-1α levels are inadequate. Therefore, the present study estimates the GCF and serum MIP-1α levels and its effects on periodontal health, disease, and after periodontal treatment.
Materials and Methods: Periodontal examination and collection of GCF and serum was performed for 60 subjects categorized into four groups with 20 subjects in each group: Group I (healthy), group II (gingivitis), and group III (chronic periodontitis). Eight weeks after scaling and root planing, the GCF and serum were collected from 20 patients of group III, who were considered as group IV. MIP-1α levels were estimated using the enzyme-linked immunosorbent assay.
Results: MIP-1α was detected in all samples. However, the levels reduced significantly in group IV (P < 0.05). Mean MIP-1α levels in GCF and serum were the highest in group III (1.481 ng/μL) and the lowest in group I (0.209 ng/μL), and those in groups II (0.685 ng/μL) and IV (0.276 ng/μL) appeared between those of groups I and III.
Conclusions: The GCF and serum MIP-1α levels increased proportionally with the progression of periodontal disease (PD) and decreased after treatment. Because MIP-1α levels in the GCF and serum correlated positively with clinical parameters, MIP-1α may be considered a “novel biomarker” in PD progression. However, controlled longitudinal studies are required to confirm this possibility. |
| first_indexed | 2024-12-11T13:28:57Z |
| format | Article |
| id | doaj.art-15d8f45270d042ff9c25ff02953a8ae8 |
| institution | Directory Open Access Journal |
| issn | 2277-8632 |
| language | English |
| last_indexed | 2024-12-11T13:28:57Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Dr. NTR University of Health Sciences |
| spelling | doaj.art-15d8f45270d042ff9c25ff02953a8ae82022-12-22T01:05:21ZengWolters Kluwer Medknow PublicationsJournal of Dr. NTR University of Health Sciences2277-86322019-01-018210711310.4103/JDRNTRUHS.JDRNTRUHS_87_18Estimation of macrophage inflammatory protein-1α (MIP-1 α) levels in serum and gingival crevicular fluid in periodontal health, disease, and after treatment—A clinico-biochemical studyMadhu Babu Dandu SubramanyamSravani Reddy CheppaliDeepa AnumlaBindu SighinamE PrasunaRavindra Nagireddy ReddyObjectives: Previous studies have revealed high macrophage inflammatory protein-1α (MIP-1α) levels in the gingival crevicular fluid (GCF) and the serum of patients with chronic periodontitis. However, reports correlating the GCF and serum MIP-1α levels are inadequate. Therefore, the present study estimates the GCF and serum MIP-1α levels and its effects on periodontal health, disease, and after periodontal treatment. Materials and Methods: Periodontal examination and collection of GCF and serum was performed for 60 subjects categorized into four groups with 20 subjects in each group: Group I (healthy), group II (gingivitis), and group III (chronic periodontitis). Eight weeks after scaling and root planing, the GCF and serum were collected from 20 patients of group III, who were considered as group IV. MIP-1α levels were estimated using the enzyme-linked immunosorbent assay. Results: MIP-1α was detected in all samples. However, the levels reduced significantly in group IV (P < 0.05). Mean MIP-1α levels in GCF and serum were the highest in group III (1.481 ng/μL) and the lowest in group I (0.209 ng/μL), and those in groups II (0.685 ng/μL) and IV (0.276 ng/μL) appeared between those of groups I and III. Conclusions: The GCF and serum MIP-1α levels increased proportionally with the progression of periodontal disease (PD) and decreased after treatment. Because MIP-1α levels in the GCF and serum correlated positively with clinical parameters, MIP-1α may be considered a “novel biomarker” in PD progression. However, controlled longitudinal studies are required to confirm this possibility.http://www.jdrntruhs.org/article.asp?issn=2277-8632;year=2019;volume=8;issue=2;spage=107;epage=113;aulast=SubramanyamGCFMIP-1αperiodontal diseasescaling and root planingserum |
| spellingShingle | Madhu Babu Dandu Subramanyam Sravani Reddy Cheppali Deepa Anumla Bindu Sighinam E Prasuna Ravindra Nagireddy Reddy Estimation of macrophage inflammatory protein-1α (MIP-1 α) levels in serum and gingival crevicular fluid in periodontal health, disease, and after treatment—A clinico-biochemical study Journal of Dr. NTR University of Health Sciences GCF MIP-1α periodontal disease scaling and root planing serum |
| title | Estimation of macrophage inflammatory protein-1α (MIP-1 α) levels in serum and gingival crevicular fluid in periodontal health, disease, and after treatment—A clinico-biochemical study |
| title_full | Estimation of macrophage inflammatory protein-1α (MIP-1 α) levels in serum and gingival crevicular fluid in periodontal health, disease, and after treatment—A clinico-biochemical study |
| title_fullStr | Estimation of macrophage inflammatory protein-1α (MIP-1 α) levels in serum and gingival crevicular fluid in periodontal health, disease, and after treatment—A clinico-biochemical study |
| title_full_unstemmed | Estimation of macrophage inflammatory protein-1α (MIP-1 α) levels in serum and gingival crevicular fluid in periodontal health, disease, and after treatment—A clinico-biochemical study |
| title_short | Estimation of macrophage inflammatory protein-1α (MIP-1 α) levels in serum and gingival crevicular fluid in periodontal health, disease, and after treatment—A clinico-biochemical study |
| title_sort | estimation of macrophage inflammatory protein 1α mip 1 α levels in serum and gingival crevicular fluid in periodontal health disease and after treatment a clinico biochemical study |
| topic | GCF MIP-1α periodontal disease scaling and root planing serum |
| url | http://www.jdrntruhs.org/article.asp?issn=2277-8632;year=2019;volume=8;issue=2;spage=107;epage=113;aulast=Subramanyam |
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