Adaptation of a Commercial NAD<sup>+</sup> Quantification Kit to Assay the Base-Exchange Activity and Substrate Preferences of SARM1

Adaptation of a Commercial NAD<sup>+</sup> Quantification Kit to Assay the Base-Exchange Activity and Substrate Preferences of SARM1

Here, we report an adapted protocol using the Promega NAD/NADH-Glo™ Assay kit. The assay normally allows quantification of trace amounts of both oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD) by enzymatic cycling, but we now show that the NAD analog 3-acetylpyridine adenine di...

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Bibliographic Details
Main Authors: Ilenia Cirilli, Adolfo Amici, Jonathan Gilley, Michael P. Coleman, Giuseppe Orsomando
Format: Article
Language:English
Published: MDPI AG 2024-02-01
Series:Molecules
Subjects:
enzyme assay
SARM1
NAD(P)ase
base exchange
pyridine bases
acetylpyridine
Online Access:https://www.mdpi.com/1420-3049/29/4/847

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