Diverse biological effects of glycosyltransferase genes from Tartary buckwheat
Abstract Background Tartary buckwheat (Fagopyrum tataricum) is an edible cereal crop whose sprouts have been marketed and commercialized for their higher levels of anti-oxidants, including rutin and anthocyanin. UDP-glucose flavonoid glycosyltransferases (UFGTs) play an important role in the biosynt...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1955-z |
| _version_ | 1818243866824802304 |
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| author | Panfeng Yao Renyu Deng Yunji Huang Simon Stael Jiaqi Shi Guanlan Shi Bingbing Lv Qi Li Qixin Dong Qi Wu Chenglei Li Hui Chen Haixia Zhao |
| author_facet | Panfeng Yao Renyu Deng Yunji Huang Simon Stael Jiaqi Shi Guanlan Shi Bingbing Lv Qi Li Qixin Dong Qi Wu Chenglei Li Hui Chen Haixia Zhao |
| author_sort | Panfeng Yao |
| collection | DOAJ |
| description | Abstract Background Tartary buckwheat (Fagopyrum tataricum) is an edible cereal crop whose sprouts have been marketed and commercialized for their higher levels of anti-oxidants, including rutin and anthocyanin. UDP-glucose flavonoid glycosyltransferases (UFGTs) play an important role in the biosynthesis of flavonoids in plants. So far, few studies are available on UFGT genes that may play a role in tartary buckwheat flavonoids biosynthesis. Here, we report on the identification and functional characterization of seven UFGTs from tartary buckwheat that are potentially involved in flavonoid biosynthesis (and have varying effects on plant growth and development when overexpressed in Arabidopsis thaliana.) Results Phylogenetic analysis indicated that the potential function of the seven FtUFGT proteins, FtUFGT6, FtUFGT7, FtUFGT8, FtUFGT9, FtUFGT15, FtUFGT40, and FtUFGT41, could be divided into three Arabidopsis thaliana functional subgroups that are involved in flavonoid biosynthesis of and anthocyanin accumulation. A significant positive correlation between FtUFGT8 and FtUFGT15 expression and anthocyanin accumulation capacity was observed in the tartary buckwheat seedlings after cold stress. Overexpression in Arabidopsis thaliana showed that FtUFGT8, FtUFGT15, and FtUFGT41 significantly increased the anthocyanin content in transgenic plants. Unexpectedly, overexpression of FtUFGT6, while not leading to enhanced anthocyanin accumulation, significantly enhanced the growth yield of transgenic plants. When wild-type plants have only cotyledons, most of the transgenic plants of FtUFGT6 had grown true leaves. Moreover, the growth speed of the oxFtUFGT6 transgenic plant root was also significantly faster than that of the wild type. At later growth, FtUFGT6 transgenic plants showed larger leaves, earlier twitching times and more tillers than wild type, whereas FtUFGT15 showed opposite results. Conclusions Seven FtUFGTs were isolated from tartary buckwheat. FtUFGT8, FtUFGT15, and FtUFGT41 can significantly increase the accumulation of total anthocyanins in transgenic plants. Furthermore, overexpression of FtUFGT6 increased the overall yield of Arabidopsis transgenic plants at all growth stages. However, FtUFGT15 shows the opposite trend at later growth stage and delays the growth speed of plants. These results suggested that the biological function of FtUFGT genes in tartary buckwheat is diverse. |
| first_indexed | 2024-12-12T14:07:56Z |
| format | Article |
| id | doaj.art-15df21c0d6e24a768546bad8fca4311a |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-12T14:07:56Z |
| publishDate | 2019-08-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-15df21c0d6e24a768546bad8fca4311a2022-12-22T00:22:10ZengBMCBMC Plant Biology1471-22292019-08-0119111510.1186/s12870-019-1955-zDiverse biological effects of glycosyltransferase genes from Tartary buckwheatPanfeng Yao0Renyu Deng1Yunji Huang2Simon Stael3Jiaqi Shi4Guanlan Shi5Bingbing Lv6Qi Li7Qixin Dong8Qi Wu9Chenglei Li10Hui Chen11Haixia Zhao12College of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityDepartment of Plant Biotechnology and Bioinformatics, Ghent UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityAbstract Background Tartary buckwheat (Fagopyrum tataricum) is an edible cereal crop whose sprouts have been marketed and commercialized for their higher levels of anti-oxidants, including rutin and anthocyanin. UDP-glucose flavonoid glycosyltransferases (UFGTs) play an important role in the biosynthesis of flavonoids in plants. So far, few studies are available on UFGT genes that may play a role in tartary buckwheat flavonoids biosynthesis. Here, we report on the identification and functional characterization of seven UFGTs from tartary buckwheat that are potentially involved in flavonoid biosynthesis (and have varying effects on plant growth and development when overexpressed in Arabidopsis thaliana.) Results Phylogenetic analysis indicated that the potential function of the seven FtUFGT proteins, FtUFGT6, FtUFGT7, FtUFGT8, FtUFGT9, FtUFGT15, FtUFGT40, and FtUFGT41, could be divided into three Arabidopsis thaliana functional subgroups that are involved in flavonoid biosynthesis of and anthocyanin accumulation. A significant positive correlation between FtUFGT8 and FtUFGT15 expression and anthocyanin accumulation capacity was observed in the tartary buckwheat seedlings after cold stress. Overexpression in Arabidopsis thaliana showed that FtUFGT8, FtUFGT15, and FtUFGT41 significantly increased the anthocyanin content in transgenic plants. Unexpectedly, overexpression of FtUFGT6, while not leading to enhanced anthocyanin accumulation, significantly enhanced the growth yield of transgenic plants. When wild-type plants have only cotyledons, most of the transgenic plants of FtUFGT6 had grown true leaves. Moreover, the growth speed of the oxFtUFGT6 transgenic plant root was also significantly faster than that of the wild type. At later growth, FtUFGT6 transgenic plants showed larger leaves, earlier twitching times and more tillers than wild type, whereas FtUFGT15 showed opposite results. Conclusions Seven FtUFGTs were isolated from tartary buckwheat. FtUFGT8, FtUFGT15, and FtUFGT41 can significantly increase the accumulation of total anthocyanins in transgenic plants. Furthermore, overexpression of FtUFGT6 increased the overall yield of Arabidopsis transgenic plants at all growth stages. However, FtUFGT15 shows the opposite trend at later growth stage and delays the growth speed of plants. These results suggested that the biological function of FtUFGT genes in tartary buckwheat is diverse.http://link.springer.com/article/10.1186/s12870-019-1955-zFlavonoids glycosyltransferaseAnthocyaninsDevelopmentTartary buckwheat |
| spellingShingle | Panfeng Yao Renyu Deng Yunji Huang Simon Stael Jiaqi Shi Guanlan Shi Bingbing Lv Qi Li Qixin Dong Qi Wu Chenglei Li Hui Chen Haixia Zhao Diverse biological effects of glycosyltransferase genes from Tartary buckwheat BMC Plant Biology Flavonoids glycosyltransferase Anthocyanins Development Tartary buckwheat |
| title | Diverse biological effects of glycosyltransferase genes from Tartary buckwheat |
| title_full | Diverse biological effects of glycosyltransferase genes from Tartary buckwheat |
| title_fullStr | Diverse biological effects of glycosyltransferase genes from Tartary buckwheat |
| title_full_unstemmed | Diverse biological effects of glycosyltransferase genes from Tartary buckwheat |
| title_short | Diverse biological effects of glycosyltransferase genes from Tartary buckwheat |
| title_sort | diverse biological effects of glycosyltransferase genes from tartary buckwheat |
| topic | Flavonoids glycosyltransferase Anthocyanins Development Tartary buckwheat |
| url | http://link.springer.com/article/10.1186/s12870-019-1955-z |
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