MicroRNA expression profile is altered in the upper airway skeletal muscle tissue of patients with obstructive sleep apnea-hypopnea syndrome
Objective To investigate the involvement of microRNAs (miRNAs) in the pathogenesis of obstructive sleep apnea-hypopnea syndrome (OSAHS). Methods In this study, we investigated miRNA profiles in the upper airway (UA) skeletal muscles of four patients with OSAHS and four matched controls using the miR...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2019-09-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/0300060519858900 |
| _version_ | 1819044961464614912 |
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| author | Jin Hou Lei Zhao Jing Yan Xiaoyong Ren Kang Zhu Tianxi Gao Xiaoying Du Huanan Luo Zhihui Li Min Xu |
| author_facet | Jin Hou Lei Zhao Jing Yan Xiaoyong Ren Kang Zhu Tianxi Gao Xiaoying Du Huanan Luo Zhihui Li Min Xu |
| author_sort | Jin Hou |
| collection | DOAJ |
| description | Objective To investigate the involvement of microRNAs (miRNAs) in the pathogenesis of obstructive sleep apnea-hypopnea syndrome (OSAHS). Methods In this study, we investigated miRNA profiles in the upper airway (UA) skeletal muscles of four patients with OSAHS and four matched controls using the miRCURY miRNA array. In another cohort of 12 OSAHS cases and 7 controls, the mRNA expression levels of interleukin (IL)-6 and Lin-28 homolog A (Lin28A), targets of the downregulated let-7 family members, were measured by real-time quantitative-PCR. The potential targets of the miRNAs were predicted by miRNA target prediction databases miRanda, Microcosm, and Targetscan. Results The array identified 370 differentially expressed miRNAs, of which 181 were upregulated and 189 were downregulated in OSAHS patients (based on a fold-change >2.0 and p < 0.05). Upregulation of IL-6 and Lin28A was validated by quantitative reverse transcription PCR. The 612 targets predicted by all three algorithms were subjected to gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. The results revealed perturbations in signaling pathways and cellular functions. Conclusion This study demonstrated profoundly altered miRNA expression profiles in upper airway muscular tissues of patients with OSAHS, which might contribute to the formation and development of OSAHS. |
| first_indexed | 2024-12-21T10:21:00Z |
| format | Article |
| id | doaj.art-15df678cee114f3abe6099e05ae01ed3 |
| institution | Directory Open Access Journal |
| issn | 0300-0605 1473-2300 |
| language | English |
| last_indexed | 2024-12-21T10:21:00Z |
| publishDate | 2019-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-15df678cee114f3abe6099e05ae01ed32022-12-21T19:07:26ZengSAGE PublishingJournal of International Medical Research0300-06051473-23002019-09-014710.1177/0300060519858900MicroRNA expression profile is altered in the upper airway skeletal muscle tissue of patients with obstructive sleep apnea-hypopnea syndromeJin HouLei ZhaoJing YanXiaoyong RenKang ZhuTianxi GaoXiaoying DuHuanan LuoZhihui LiMin XuObjective To investigate the involvement of microRNAs (miRNAs) in the pathogenesis of obstructive sleep apnea-hypopnea syndrome (OSAHS). Methods In this study, we investigated miRNA profiles in the upper airway (UA) skeletal muscles of four patients with OSAHS and four matched controls using the miRCURY miRNA array. In another cohort of 12 OSAHS cases and 7 controls, the mRNA expression levels of interleukin (IL)-6 and Lin-28 homolog A (Lin28A), targets of the downregulated let-7 family members, were measured by real-time quantitative-PCR. The potential targets of the miRNAs were predicted by miRNA target prediction databases miRanda, Microcosm, and Targetscan. Results The array identified 370 differentially expressed miRNAs, of which 181 were upregulated and 189 were downregulated in OSAHS patients (based on a fold-change >2.0 and p < 0.05). Upregulation of IL-6 and Lin28A was validated by quantitative reverse transcription PCR. The 612 targets predicted by all three algorithms were subjected to gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. The results revealed perturbations in signaling pathways and cellular functions. Conclusion This study demonstrated profoundly altered miRNA expression profiles in upper airway muscular tissues of patients with OSAHS, which might contribute to the formation and development of OSAHS.https://doi.org/10.1177/0300060519858900 |
| spellingShingle | Jin Hou Lei Zhao Jing Yan Xiaoyong Ren Kang Zhu Tianxi Gao Xiaoying Du Huanan Luo Zhihui Li Min Xu MicroRNA expression profile is altered in the upper airway skeletal muscle tissue of patients with obstructive sleep apnea-hypopnea syndrome Journal of International Medical Research |
| title | MicroRNA expression profile is altered in the upper airway skeletal muscle tissue of patients with obstructive sleep apnea-hypopnea syndrome |
| title_full | MicroRNA expression profile is altered in the upper airway skeletal muscle tissue of patients with obstructive sleep apnea-hypopnea syndrome |
| title_fullStr | MicroRNA expression profile is altered in the upper airway skeletal muscle tissue of patients with obstructive sleep apnea-hypopnea syndrome |
| title_full_unstemmed | MicroRNA expression profile is altered in the upper airway skeletal muscle tissue of patients with obstructive sleep apnea-hypopnea syndrome |
| title_short | MicroRNA expression profile is altered in the upper airway skeletal muscle tissue of patients with obstructive sleep apnea-hypopnea syndrome |
| title_sort | microrna expression profile is altered in the upper airway skeletal muscle tissue of patients with obstructive sleep apnea hypopnea syndrome |
| url | https://doi.org/10.1177/0300060519858900 |
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