Genetic variability for coloured caryopses in common wheat varieties determined by microsatellite markers
Products made from wheat are the most important components of the human diet, and could also become a source of functional foods and feed ingredients, e.g. minerals, vitamins and/or phytochemicals. The caryopses of certain wheat genotypes contain antioxidants, i.e. anthocyanins or carotenoids, which...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2013-09-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
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| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201303-0004_genetic-variability-for-coloured-caryopses-in-common-wheat-varieties-determined-by-microsatellite-markers.php |
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| author | Milena MUSILOVÁ Václav TROJAN Tomáš VYHNÁNEK Ladislav HAVEL |
| author_facet | Milena MUSILOVÁ Václav TROJAN Tomáš VYHNÁNEK Ladislav HAVEL |
| author_sort | Milena MUSILOVÁ |
| collection | DOAJ |
| description | Products made from wheat are the most important components of the human diet, and could also become a source of functional foods and feed ingredients, e.g. minerals, vitamins and/or phytochemicals. The caryopses of certain wheat genotypes contain antioxidants, i.e. anthocyanins or carotenoids, which cause purple, blue or yellow coloration. The first step before the introduction of these traits into individual wheat cultivars is the characterization of relationships and the possibility of new gene combinations. In this study, relationships among 24 genotypes with different types of caryopsis colour were investigated by means of microsatellite markers. Using 44 SSR (Simple Sequence Repeat) markers it was possible to detect a total of 184 alleles; on average, approximately 4 alleles were detected at a microsatellite locus. Using a set of 5 SSR markers (Xgwm636, Xbarc077, Xwmc262, Xgwm397 and Xwmc219) with PIC (polymorphic information content) values higher than 0.70, it was possible to differentiate among all the genotypes analysed. A dendrogram was created on the basis of all SSR markers, and showed that the genotypes were divided into two groups. Three, and one genotype with purple and blue caryopsis, respectively, belonged to one cluster, while the remaining twenty formed the second, greater cluster, which was subdivided into 2 sub-clusters: one of them involved genotypes with blue caryopses, and the other those with yellow and red caryopses. The genotype of tall wheatgrass (Thinopyrum ponticum), as a possible donor of genes responsible for blue caryopses, was also classified. These results can be used in wheat breeding programmes aimed at the selection of functional foodstuffs. |
| first_indexed | 2024-04-10T08:29:05Z |
| format | Article |
| id | doaj.art-15dfcfdf0b6a4b2ebedd9938040ab968 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:29:05Z |
| publishDate | 2013-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-15dfcfdf0b6a4b2ebedd9938040ab9682023-02-23T03:30:18ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252013-09-0149311612210.17221/160/2012-CJGPBcjg-201303-0004Genetic variability for coloured caryopses in common wheat varieties determined by microsatellite markersMilena MUSILOVÁ0Václav TROJAN1Tomáš VYHNÁNEK2Ladislav HAVEL3Department of Plant Biology, Faculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicDepartment of Plant Biology, Faculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicDepartment of Plant Biology, Faculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicDepartment of Plant Biology, Faculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicProducts made from wheat are the most important components of the human diet, and could also become a source of functional foods and feed ingredients, e.g. minerals, vitamins and/or phytochemicals. The caryopses of certain wheat genotypes contain antioxidants, i.e. anthocyanins or carotenoids, which cause purple, blue or yellow coloration. The first step before the introduction of these traits into individual wheat cultivars is the characterization of relationships and the possibility of new gene combinations. In this study, relationships among 24 genotypes with different types of caryopsis colour were investigated by means of microsatellite markers. Using 44 SSR (Simple Sequence Repeat) markers it was possible to detect a total of 184 alleles; on average, approximately 4 alleles were detected at a microsatellite locus. Using a set of 5 SSR markers (Xgwm636, Xbarc077, Xwmc262, Xgwm397 and Xwmc219) with PIC (polymorphic information content) values higher than 0.70, it was possible to differentiate among all the genotypes analysed. A dendrogram was created on the basis of all SSR markers, and showed that the genotypes were divided into two groups. Three, and one genotype with purple and blue caryopsis, respectively, belonged to one cluster, while the remaining twenty formed the second, greater cluster, which was subdivided into 2 sub-clusters: one of them involved genotypes with blue caryopses, and the other those with yellow and red caryopses. The genotype of tall wheatgrass (Thinopyrum ponticum), as a possible donor of genes responsible for blue caryopses, was also classified. These results can be used in wheat breeding programmes aimed at the selection of functional foodstuffs.https://cjgpb.agriculturejournals.cz/artkey/cjg-201303-0004_genetic-variability-for-coloured-caryopses-in-common-wheat-varieties-determined-by-microsatellite-markers.phpaleurone layerendospermgrainpericarpssr markerstriticum aestivumthinopyrum ponticum |
| spellingShingle | Milena MUSILOVÁ Václav TROJAN Tomáš VYHNÁNEK Ladislav HAVEL Genetic variability for coloured caryopses in common wheat varieties determined by microsatellite markers Czech Journal of Genetics and Plant Breeding aleurone layer endosperm grain pericarp ssr markers triticum aestivum thinopyrum ponticum |
| title | Genetic variability for coloured caryopses in common wheat varieties determined by microsatellite markers |
| title_full | Genetic variability for coloured caryopses in common wheat varieties determined by microsatellite markers |
| title_fullStr | Genetic variability for coloured caryopses in common wheat varieties determined by microsatellite markers |
| title_full_unstemmed | Genetic variability for coloured caryopses in common wheat varieties determined by microsatellite markers |
| title_short | Genetic variability for coloured caryopses in common wheat varieties determined by microsatellite markers |
| title_sort | genetic variability for coloured caryopses in common wheat varieties determined by microsatellite markers |
| topic | aleurone layer endosperm grain pericarp ssr markers triticum aestivum thinopyrum ponticum |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201303-0004_genetic-variability-for-coloured-caryopses-in-common-wheat-varieties-determined-by-microsatellite-markers.php |
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