Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine
Nanoconstructs intended to be used as biomedical tool must be assessed for their capability to cross biological barriers. However, studying in vivo the permeability of biological barriers to nanoparticles is quite difficult due to the many structural and functional factors involved. Therefore, the...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2022-11-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | https://www.ejh.it/index.php/ejh/article/view/3603 |
| _version_ | 1811305188074455040 |
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| author | Flavia Carton Manuela Malatesta |
| author_facet | Flavia Carton Manuela Malatesta |
| author_sort | Flavia Carton |
| collection | DOAJ |
| description |
Nanoconstructs intended to be used as biomedical tool must be assessed for their capability to cross biological barriers. However, studying in vivo the permeability of biological barriers to nanoparticles is quite difficult due to the many structural and functional factors involved. Therefore, the in vitro modeling of biological barriers -2D cell monocultures, 2D/3D cell co-cultures, microfluidic devices- is gaining more and more relevance in nanomedical research. Microscopy techniques play a crucial role in these studies, as they allow both visualizing nanoparticles inside the biological barrier and evaluating their impact on the barrier components. This paper provides an overview of the various microscopical approaches used to investigate nanoparticle translocation through in vitro biological barrier models. The high number of scientific articles reported highlights the great contribution of the morphological and histochemical approach to the knowledge of the dynamic interactions between nanoconstructs and the living environment.
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| first_indexed | 2024-04-13T08:20:55Z |
| format | Article |
| id | doaj.art-15e4c42680f34f7a9b2a657c7389b409 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-04-13T08:20:55Z |
| publishDate | 2022-11-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-15e4c42680f34f7a9b2a657c7389b4092022-12-22T02:54:38ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062022-11-0166410.4081/ejh.2022.3603Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicineFlavia Carton0Manuela Malatesta1Department of Health Sciences, University of Piemonte Orientale “A. Avogadro”, NovaraDepartment of Neurosciences, Biomedicine and Movement Sciences, Anatomy and Histology Section, University of Verona Nanoconstructs intended to be used as biomedical tool must be assessed for their capability to cross biological barriers. However, studying in vivo the permeability of biological barriers to nanoparticles is quite difficult due to the many structural and functional factors involved. Therefore, the in vitro modeling of biological barriers -2D cell monocultures, 2D/3D cell co-cultures, microfluidic devices- is gaining more and more relevance in nanomedical research. Microscopy techniques play a crucial role in these studies, as they allow both visualizing nanoparticles inside the biological barrier and evaluating their impact on the barrier components. This paper provides an overview of the various microscopical approaches used to investigate nanoparticle translocation through in vitro biological barrier models. The high number of scientific articles reported highlights the great contribution of the morphological and histochemical approach to the knowledge of the dynamic interactions between nanoconstructs and the living environment. https://www.ejh.it/index.php/ejh/article/view/3603nanoconstructscell cultureorganoidmicrofluidicsbioreactorfluorescence microscopy |
| spellingShingle | Flavia Carton Manuela Malatesta Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine European Journal of Histochemistry nanoconstructs cell culture organoid microfluidics bioreactor fluorescence microscopy |
| title | Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine |
| title_full | Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine |
| title_fullStr | Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine |
| title_full_unstemmed | Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine |
| title_short | Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine |
| title_sort | assessing the interactions between nanoparticles and biological barriers in vitro a new challenge for microscopy techniques in nanomedicine |
| topic | nanoconstructs cell culture organoid microfluidics bioreactor fluorescence microscopy |
| url | https://www.ejh.it/index.php/ejh/article/view/3603 |
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