The Influence of Polyanions and Polycations on Bacteriophage Activity
Phage therapy is a great alternative to antibiotic drugs, but it can’t effectively overcome the over-acidic medium of the stomach. We offer the use of polyelectrolyte microcapsules as a protective means of bacteriophage. It is necessary to understand the influence of polyelectrolytes on bacteriophag...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/6/914 |
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| author | Egor V. Musin Aleksandr L. Kim Alexey V. Dubrovskii Ekaterina B. Kudryashova Elena V. Ariskina Sergey A. Tikhonenko |
| author_facet | Egor V. Musin Aleksandr L. Kim Alexey V. Dubrovskii Ekaterina B. Kudryashova Elena V. Ariskina Sergey A. Tikhonenko |
| author_sort | Egor V. Musin |
| collection | DOAJ |
| description | Phage therapy is a great alternative to antibiotic drugs, but it can’t effectively overcome the over-acidic medium of the stomach. We offer the use of polyelectrolyte microcapsules as a protective means of bacteriophage. It is necessary to understand the influence of polyelectrolytes on bacteriophage survival. The work studied the effect of polyanions and polycations on the coliprotetic bacteriophage’s viability. We have shown that polyallylamine decreased bacteriophage’s viability during increasing polyelectrolyte concentration and polyarginine had a lower inhibitory effect (then PAH) on the activity of the bacteriophage due to polyelectrolyte concentration from 0.05 to 5 mg/mL. It was shown that the inhibition of the bacteriophage by polyallylamine had an electrostatic nature and the use of high ionic strength prevented the formation of the PAH-protein capsid complex. Polystyrene sulfonate does not affect bacteriophage viability during increasing polyelectrolyte concentration from 0.05 mg/mL to 1 mg/mL. Polystyrene sulfonate decreases the viability of bacteriophage from 5 mg/mL of polyelectrolyte concentration. Dextran sulfate inhibits bacteriophage activity at 20–30%. Dextran inhibits bacteriophage activity by 80% at diapason concentration from 0.05 to 5 mg/mL and loses the inhibition effect from a concentration of 5 mg/mL. |
| first_indexed | 2024-03-10T13:11:17Z |
| format | Article |
| id | doaj.art-15e84bc200eb43db8e32ef1b3ab8065c |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T13:11:17Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-15e84bc200eb43db8e32ef1b3ab8065c2023-11-21T10:44:55ZengMDPI AGPolymers2073-43602021-03-0113691410.3390/polym13060914The Influence of Polyanions and Polycations on Bacteriophage ActivityEgor V. Musin0Aleksandr L. Kim1Alexey V. Dubrovskii2Ekaterina B. Kudryashova3Elena V. Ariskina4Sergey A. Tikhonenko5Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Institutskaya st. 3, 142290 Puschino, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Science, Institutskaya st. 3, 142290 Puschino, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Science, Institutskaya st. 3, 142290 Puschino, RussiaG. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Prospect Nauki 5, 142290 Pushchino, RussiaG. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Prospect Nauki 5, 142290 Pushchino, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Science, Institutskaya st. 3, 142290 Puschino, RussiaPhage therapy is a great alternative to antibiotic drugs, but it can’t effectively overcome the over-acidic medium of the stomach. We offer the use of polyelectrolyte microcapsules as a protective means of bacteriophage. It is necessary to understand the influence of polyelectrolytes on bacteriophage survival. The work studied the effect of polyanions and polycations on the coliprotetic bacteriophage’s viability. We have shown that polyallylamine decreased bacteriophage’s viability during increasing polyelectrolyte concentration and polyarginine had a lower inhibitory effect (then PAH) on the activity of the bacteriophage due to polyelectrolyte concentration from 0.05 to 5 mg/mL. It was shown that the inhibition of the bacteriophage by polyallylamine had an electrostatic nature and the use of high ionic strength prevented the formation of the PAH-protein capsid complex. Polystyrene sulfonate does not affect bacteriophage viability during increasing polyelectrolyte concentration from 0.05 mg/mL to 1 mg/mL. Polystyrene sulfonate decreases the viability of bacteriophage from 5 mg/mL of polyelectrolyte concentration. Dextran sulfate inhibits bacteriophage activity at 20–30%. Dextran inhibits bacteriophage activity by 80% at diapason concentration from 0.05 to 5 mg/mL and loses the inhibition effect from a concentration of 5 mg/mL.https://www.mdpi.com/2073-4360/13/6/914bacteriophagespolyelectrolytespolyallylaminepolysteryne sulfonatedextrane sulfatepolyarginine |
| spellingShingle | Egor V. Musin Aleksandr L. Kim Alexey V. Dubrovskii Ekaterina B. Kudryashova Elena V. Ariskina Sergey A. Tikhonenko The Influence of Polyanions and Polycations on Bacteriophage Activity Polymers bacteriophages polyelectrolytes polyallylamine polysteryne sulfonate dextrane sulfate polyarginine |
| title | The Influence of Polyanions and Polycations on Bacteriophage Activity |
| title_full | The Influence of Polyanions and Polycations on Bacteriophage Activity |
| title_fullStr | The Influence of Polyanions and Polycations on Bacteriophage Activity |
| title_full_unstemmed | The Influence of Polyanions and Polycations on Bacteriophage Activity |
| title_short | The Influence of Polyanions and Polycations on Bacteriophage Activity |
| title_sort | influence of polyanions and polycations on bacteriophage activity |
| topic | bacteriophages polyelectrolytes polyallylamine polysteryne sulfonate dextrane sulfate polyarginine |
| url | https://www.mdpi.com/2073-4360/13/6/914 |
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