The autistic-like behaviors development during weaning and sexual maturation in VPA-induced autistic-like rats is accompanied by gut microbiota dysbiosis
Researches on gut microbiota in autism have mostly focused on children, but the dynamic changes of gut microbiota from weaning to adulthood were still not clear because of the difficulty of diagnosing autism. In this study, autistic-like rats indued by valproate (VPA) were tracked from weaning (end...
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PeerJ Inc.
2021-05-01
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| author | Qingmin Kong Peijun Tian Jianxin Zhao Hao Zhang Gang Wang Wei Chen |
| author_facet | Qingmin Kong Peijun Tian Jianxin Zhao Hao Zhang Gang Wang Wei Chen |
| author_sort | Qingmin Kong |
| collection | DOAJ |
| description | Researches on gut microbiota in autism have mostly focused on children, but the dynamic changes of gut microbiota from weaning to adulthood were still not clear because of the difficulty of diagnosing autism. In this study, autistic-like rats indued by valproate (VPA) were tracked from weaning (end of breastfeeding; four weeks old) to sexual maturation (food; eight weeks old). Autistic-like rats were found to show obvious developmental disorders. During weaning, autistic-like rats only exhibited obvious repetitive stereotyped behaviors, but the autistic-like behaviors were fully apparent upon sexual maturation. Significant differences were observed between the gut microbiota of autistic-like and healthy rats across both age groups. The correlation analysis results revealed that the correlation between behaviors and some microbiota, especially Helicobacter, did not vary with age or diet. The total amount of short-chain fatty acids (SCFAs) decreased, butyric acid metabolism decreased, and propionic acid metabolism increased in the feces of autistic-like rats. The correlation between autistic-like behaviors and the butyric acid and propionic acid levels did not vary with diet or age. Inositol phosphate metabolism, amino acid metabolism, and lipopolysaccharide biosynthesis were significantly associated with autistic-like behaviors. Our results showed that although the microbiota and SCFAs related to autism were affected by age and diet, some remained consistent irrespective of age and diet, and they could be considered two of the factors related to autistic-like behaviors development. |
| first_indexed | 2024-03-09T06:36:53Z |
| format | Article |
| id | doaj.art-15ed5c07228c487da738323f48dc1fe1 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:36:53Z |
| publishDate | 2021-05-01 |
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| spelling | doaj.art-15ed5c07228c487da738323f48dc1fe12023-12-03T10:55:50ZengPeerJ Inc.PeerJ2167-83592021-05-019e1110310.7717/peerj.11103The autistic-like behaviors development during weaning and sexual maturation in VPA-induced autistic-like rats is accompanied by gut microbiota dysbiosisQingmin Kong0Peijun Tian1Jianxin Zhao2Hao Zhang3Gang Wang4Wei Chen5State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P. R. ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P. R. ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P. R. ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P. R. ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P. R. ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P. R. ChinaResearches on gut microbiota in autism have mostly focused on children, but the dynamic changes of gut microbiota from weaning to adulthood were still not clear because of the difficulty of diagnosing autism. In this study, autistic-like rats indued by valproate (VPA) were tracked from weaning (end of breastfeeding; four weeks old) to sexual maturation (food; eight weeks old). Autistic-like rats were found to show obvious developmental disorders. During weaning, autistic-like rats only exhibited obvious repetitive stereotyped behaviors, but the autistic-like behaviors were fully apparent upon sexual maturation. Significant differences were observed between the gut microbiota of autistic-like and healthy rats across both age groups. The correlation analysis results revealed that the correlation between behaviors and some microbiota, especially Helicobacter, did not vary with age or diet. The total amount of short-chain fatty acids (SCFAs) decreased, butyric acid metabolism decreased, and propionic acid metabolism increased in the feces of autistic-like rats. The correlation between autistic-like behaviors and the butyric acid and propionic acid levels did not vary with diet or age. Inositol phosphate metabolism, amino acid metabolism, and lipopolysaccharide biosynthesis were significantly associated with autistic-like behaviors. Our results showed that although the microbiota and SCFAs related to autism were affected by age and diet, some remained consistent irrespective of age and diet, and they could be considered two of the factors related to autistic-like behaviors development.https://peerj.com/articles/11103.pdfAutism spectrum disorderWeaningSexual maturationBehavioristicsGutmicrobiota |
| spellingShingle | Qingmin Kong Peijun Tian Jianxin Zhao Hao Zhang Gang Wang Wei Chen The autistic-like behaviors development during weaning and sexual maturation in VPA-induced autistic-like rats is accompanied by gut microbiota dysbiosis PeerJ Autism spectrum disorder Weaning Sexual maturation Behavioristics Gutmicrobiota |
| title | The autistic-like behaviors development during weaning and sexual maturation in VPA-induced autistic-like rats is accompanied by gut microbiota dysbiosis |
| title_full | The autistic-like behaviors development during weaning and sexual maturation in VPA-induced autistic-like rats is accompanied by gut microbiota dysbiosis |
| title_fullStr | The autistic-like behaviors development during weaning and sexual maturation in VPA-induced autistic-like rats is accompanied by gut microbiota dysbiosis |
| title_full_unstemmed | The autistic-like behaviors development during weaning and sexual maturation in VPA-induced autistic-like rats is accompanied by gut microbiota dysbiosis |
| title_short | The autistic-like behaviors development during weaning and sexual maturation in VPA-induced autistic-like rats is accompanied by gut microbiota dysbiosis |
| title_sort | autistic like behaviors development during weaning and sexual maturation in vpa induced autistic like rats is accompanied by gut microbiota dysbiosis |
| topic | Autism spectrum disorder Weaning Sexual maturation Behavioristics Gutmicrobiota |
| url | https://peerj.com/articles/11103.pdf |
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