Intraarticular injection of processed lipoaspirate cells has anti-inflammatory and analgesic effects but does not improve degenerative changes in murine monoiodoacetate-induced osteoarthritis
Abstract Background Previous basic research and clinical studies examined the effects of mesenchymal stem cells (MSCs) on regeneration and maintenance of articular cartilage. However, our pilot study suggested that MSCs are more effective at suppressing inflammation and pain rather than promoting ca...
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | BMC Musculoskeletal Disorders |
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| Online Access: | http://link.springer.com/article/10.1186/s12891-019-2710-1 |
| _version_ | 1818973385996107776 |
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| author | Takumi Sakamoto Tsuyoshi Miyazaki Shuji Watanabe Ai Takahashi Kazuya Honjoh Hideaki Nakajima Hisashi Oki Yasuo Kokubo Akihiko Matsumine |
| author_facet | Takumi Sakamoto Tsuyoshi Miyazaki Shuji Watanabe Ai Takahashi Kazuya Honjoh Hideaki Nakajima Hisashi Oki Yasuo Kokubo Akihiko Matsumine |
| author_sort | Takumi Sakamoto |
| collection | DOAJ |
| description | Abstract Background Previous basic research and clinical studies examined the effects of mesenchymal stem cells (MSCs) on regeneration and maintenance of articular cartilage. However, our pilot study suggested that MSCs are more effective at suppressing inflammation and pain rather than promoting cartilage regeneration in osteoarthritis. Adipose tissue is considered a useful source of MSCs; it can be harvested easily in larger quantities compared with the bone marrow. The present study was designed to evaluate the anti-inflammatory, analgesic, and regenerative effects of intra-articularly injected processed lipoaspirate (PLA) cells (containing adipose-derived MSCs) on degenerative cartilage in a rat osteoarthritis model. Methods PLA cells were isolated from subcutaneous adipose tissue of 12-week-old female Sprague-Dawley rats. Osteoarthritis was induced by injection of monoiodoacetate (MIA). Each rat received 1 × 106 MSCs into the joint at day 7 (early injection group) and day 14 (late injection group) post-MIA injection. At 7, 14, 21 days after MIA administration, pain was assessed by immunostaining and western blotting of dorsal root ganglion (DRG). Cartilage quality was assessed macroscopically and by safranin-O and H&E staining, and joint inflammation was assessed by western blotting of the synovium. Results The early injection group showed less cartilage degradation, whereas the late injection group showed cartilage damage similar to untreated OA group. The relative expression level of CGRP protein in DRG neurons was significantly lower in the two treatment groups, compared with the untreated group. Conclusions Intra-articular injection of PLA cells prevented degenerative changes in the early injection group, but had little effect in promoting cartilage repair in the late injection group. Interestingly, intra-articular injection of PLA cells resulted in suppression of inflammation and pain in both OA groups. Further studies are needed to determine the long-term effects of intra-articular injection of PLA cells in osteoarthritis. |
| first_indexed | 2024-12-20T15:23:20Z |
| format | Article |
| id | doaj.art-15ee7601a938487f983d14842ae03ea3 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-20T15:23:20Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-15ee7601a938487f983d14842ae03ea32022-12-21T19:35:57ZengBMCBMC Musculoskeletal Disorders1471-24742019-07-0120111110.1186/s12891-019-2710-1Intraarticular injection of processed lipoaspirate cells has anti-inflammatory and analgesic effects but does not improve degenerative changes in murine monoiodoacetate-induced osteoarthritisTakumi Sakamoto0Tsuyoshi Miyazaki1Shuji Watanabe2Ai Takahashi3Kazuya Honjoh4Hideaki Nakajima5Hisashi Oki6Yasuo Kokubo7Akihiko Matsumine8Department of Orthopaedics and Rehabilitation Medicine, Faculty of Medical Sciences, University of FukuiDepartment of Orthopaedics and Rehabilitation Medicine, Faculty of Medical Sciences, University of FukuiDepartment of Orthopaedics and Rehabilitation Medicine, Faculty of Medical Sciences, University of FukuiDepartment of Orthopaedics and Rehabilitation Medicine, Faculty of Medical Sciences, University of FukuiDepartment of Orthopaedics and Rehabilitation Medicine, Faculty of Medical Sciences, University of FukuiDepartment of Orthopaedics and Rehabilitation Medicine, Faculty of Medical Sciences, University of FukuiDepartment of Orthopaedics and Rehabilitation Medicine, Faculty of Medical Sciences, University of FukuiDepartment of Orthopaedics and Rehabilitation Medicine, Faculty of Medical Sciences, University of FukuiDepartment of Orthopaedics and Rehabilitation Medicine, Faculty of Medical Sciences, University of FukuiAbstract Background Previous basic research and clinical studies examined the effects of mesenchymal stem cells (MSCs) on regeneration and maintenance of articular cartilage. However, our pilot study suggested that MSCs are more effective at suppressing inflammation and pain rather than promoting cartilage regeneration in osteoarthritis. Adipose tissue is considered a useful source of MSCs; it can be harvested easily in larger quantities compared with the bone marrow. The present study was designed to evaluate the anti-inflammatory, analgesic, and regenerative effects of intra-articularly injected processed lipoaspirate (PLA) cells (containing adipose-derived MSCs) on degenerative cartilage in a rat osteoarthritis model. Methods PLA cells were isolated from subcutaneous adipose tissue of 12-week-old female Sprague-Dawley rats. Osteoarthritis was induced by injection of monoiodoacetate (MIA). Each rat received 1 × 106 MSCs into the joint at day 7 (early injection group) and day 14 (late injection group) post-MIA injection. At 7, 14, 21 days after MIA administration, pain was assessed by immunostaining and western blotting of dorsal root ganglion (DRG). Cartilage quality was assessed macroscopically and by safranin-O and H&E staining, and joint inflammation was assessed by western blotting of the synovium. Results The early injection group showed less cartilage degradation, whereas the late injection group showed cartilage damage similar to untreated OA group. The relative expression level of CGRP protein in DRG neurons was significantly lower in the two treatment groups, compared with the untreated group. Conclusions Intra-articular injection of PLA cells prevented degenerative changes in the early injection group, but had little effect in promoting cartilage repair in the late injection group. Interestingly, intra-articular injection of PLA cells resulted in suppression of inflammation and pain in both OA groups. Further studies are needed to determine the long-term effects of intra-articular injection of PLA cells in osteoarthritis.http://link.springer.com/article/10.1186/s12891-019-2710-1Processed lipoaspirate (PLA) cellsMesenchymal stem cells (MSCs)Adipose derived-mesenchymal stem cells (AD-MSCs)Intra-articular injectionOsteoarthritis (OA)Monoiodoacetate (MIA) |
| spellingShingle | Takumi Sakamoto Tsuyoshi Miyazaki Shuji Watanabe Ai Takahashi Kazuya Honjoh Hideaki Nakajima Hisashi Oki Yasuo Kokubo Akihiko Matsumine Intraarticular injection of processed lipoaspirate cells has anti-inflammatory and analgesic effects but does not improve degenerative changes in murine monoiodoacetate-induced osteoarthritis BMC Musculoskeletal Disorders Processed lipoaspirate (PLA) cells Mesenchymal stem cells (MSCs) Adipose derived-mesenchymal stem cells (AD-MSCs) Intra-articular injection Osteoarthritis (OA) Monoiodoacetate (MIA) |
| title | Intraarticular injection of processed lipoaspirate cells has anti-inflammatory and analgesic effects but does not improve degenerative changes in murine monoiodoacetate-induced osteoarthritis |
| title_full | Intraarticular injection of processed lipoaspirate cells has anti-inflammatory and analgesic effects but does not improve degenerative changes in murine monoiodoacetate-induced osteoarthritis |
| title_fullStr | Intraarticular injection of processed lipoaspirate cells has anti-inflammatory and analgesic effects but does not improve degenerative changes in murine monoiodoacetate-induced osteoarthritis |
| title_full_unstemmed | Intraarticular injection of processed lipoaspirate cells has anti-inflammatory and analgesic effects but does not improve degenerative changes in murine monoiodoacetate-induced osteoarthritis |
| title_short | Intraarticular injection of processed lipoaspirate cells has anti-inflammatory and analgesic effects but does not improve degenerative changes in murine monoiodoacetate-induced osteoarthritis |
| title_sort | intraarticular injection of processed lipoaspirate cells has anti inflammatory and analgesic effects but does not improve degenerative changes in murine monoiodoacetate induced osteoarthritis |
| topic | Processed lipoaspirate (PLA) cells Mesenchymal stem cells (MSCs) Adipose derived-mesenchymal stem cells (AD-MSCs) Intra-articular injection Osteoarthritis (OA) Monoiodoacetate (MIA) |
| url | http://link.springer.com/article/10.1186/s12891-019-2710-1 |
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