Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays
Shiga toxin-producing Escherichia coli O157:H7 (STEC) cause food-borne illness that may be fatal. STEC strains enumerate two types of potent Shiga toxins (Stx1 and Stx2) that are responsible for causing diseases. It is important to detect the E. coli O157 and Shiga toxins in food to prevent outbreak...
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| Format: | Article |
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MDPI AG
2016-03-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/8/4/92 |
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| author | Jinliang Wang Robab Katani Lingling Li Narasimha Hegde Elisabeth L. Roberts Vivek Kapur Chitrita DebRoy |
| author_facet | Jinliang Wang Robab Katani Lingling Li Narasimha Hegde Elisabeth L. Roberts Vivek Kapur Chitrita DebRoy |
| author_sort | Jinliang Wang |
| collection | DOAJ |
| description | Shiga toxin-producing Escherichia coli O157:H7 (STEC) cause food-borne illness that may be fatal. STEC strains enumerate two types of potent Shiga toxins (Stx1 and Stx2) that are responsible for causing diseases. It is important to detect the E. coli O157 and Shiga toxins in food to prevent outbreak of diseases. We describe the development of two multi-analyte antibody-based lateral flow immunoassays (LFIA); one for the detection of Stx1 and Stx2 and one for the detection of E. coli O157 that may be used simultaneously to detect pathogenic E. coli O157:H7. The LFIA strips were developed by conjugating nano colloidal gold particles with monoclonal antibodies against Stx1 and Stx2 and anti-lipid A antibodies to capture Shiga toxins and O157 antigen, respectively. Our results indicate that the LFIA for Stx is highly specific and detected Stx1 and Stx2 within three hours of induction of STEC with ciprofloxacin at 37 °C. The limit of detection for E. coli O157 LFIA was found to be 105 CFU/mL in ground beef spiked with the pathogen. The LFIAs are rapid, accurate and easy to use and do not require sophisticated equipment or trained personnel. Following the assay, colored bands on the membrane develop for end-point detection. The LFIAs may be used for screening STEC in food and the environment. |
| first_indexed | 2024-04-14T03:38:21Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T03:38:21Z |
| publishDate | 2016-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-15f8b76495d84d34ac34a302231a76a32022-12-22T02:14:39ZengMDPI AGToxins2072-66512016-03-01849210.3390/toxins8040092toxins8040092Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow ImmunoassaysJinliang Wang0Robab Katani1Lingling Li2Narasimha Hegde3Elisabeth L. Roberts4Vivek Kapur5Chitrita DebRoy6Present address: Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, China.Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, 115 Henning Building, University Park, PA 16802, USADepartment of Veterinary and Biomedical Sciences, The Pennsylvania State University, 115 Henning Building, University Park, PA 16802, USADepartment of Veterinary and Biomedical Sciences, The Pennsylvania State University, 115 Henning Building, University Park, PA 16802, USADepartment of Veterinary and Biomedical Sciences, The Pennsylvania State University, 115 Henning Building, University Park, PA 16802, USADepartment of Veterinary and Biomedical Sciences, The Pennsylvania State University, 115 Henning Building, University Park, PA 16802, USADepartment of Veterinary and Biomedical Sciences, The Pennsylvania State University, 115 Henning Building, University Park, PA 16802, USAShiga toxin-producing Escherichia coli O157:H7 (STEC) cause food-borne illness that may be fatal. STEC strains enumerate two types of potent Shiga toxins (Stx1 and Stx2) that are responsible for causing diseases. It is important to detect the E. coli O157 and Shiga toxins in food to prevent outbreak of diseases. We describe the development of two multi-analyte antibody-based lateral flow immunoassays (LFIA); one for the detection of Stx1 and Stx2 and one for the detection of E. coli O157 that may be used simultaneously to detect pathogenic E. coli O157:H7. The LFIA strips were developed by conjugating nano colloidal gold particles with monoclonal antibodies against Stx1 and Stx2 and anti-lipid A antibodies to capture Shiga toxins and O157 antigen, respectively. Our results indicate that the LFIA for Stx is highly specific and detected Stx1 and Stx2 within three hours of induction of STEC with ciprofloxacin at 37 °C. The limit of detection for E. coli O157 LFIA was found to be 105 CFU/mL in ground beef spiked with the pathogen. The LFIAs are rapid, accurate and easy to use and do not require sophisticated equipment or trained personnel. Following the assay, colored bands on the membrane develop for end-point detection. The LFIAs may be used for screening STEC in food and the environment.http://www.mdpi.com/2072-6651/8/4/92lateral flow immunoassay (LFIA)colloidal goldE. coli O157Shiga toxinStxSTECfood safety |
| spellingShingle | Jinliang Wang Robab Katani Lingling Li Narasimha Hegde Elisabeth L. Roberts Vivek Kapur Chitrita DebRoy Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays Toxins lateral flow immunoassay (LFIA) colloidal gold E. coli O157 Shiga toxin Stx STEC food safety |
| title | Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays |
| title_full | Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays |
| title_fullStr | Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays |
| title_full_unstemmed | Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays |
| title_short | Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays |
| title_sort | rapid detection of escherichia coli o157 and shiga toxins by lateral flow immunoassays |
| topic | lateral flow immunoassay (LFIA) colloidal gold E. coli O157 Shiga toxin Stx STEC food safety |
| url | http://www.mdpi.com/2072-6651/8/4/92 |
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