Nucleus Near-Infrared (nNIR) Irradiation of Single A549 Cells Induces DNA Damage and Activates EGFR Leading to Mitochondrial Fission
There has been great interest in identifying the biological substrate for light-cell interaction and their relations to cancer treatment. In this study, a near-infrared (NIR) laser is focused into the nucleus (nNIR) or cytoplasm (cNIR) of a single living cell by a high numerical aperture condenser t...
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MDPI AG
2022-02-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/4/624 |
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| author | Momoh Gbetuwa Long-Sheng Lu Tsung-Jen Wang Yin-Ju Chen Jeng-Fong Chiou Tai-Yuan Su Tzu-Sen Yang |
| author_facet | Momoh Gbetuwa Long-Sheng Lu Tsung-Jen Wang Yin-Ju Chen Jeng-Fong Chiou Tai-Yuan Su Tzu-Sen Yang |
| author_sort | Momoh Gbetuwa |
| collection | DOAJ |
| description | There has been great interest in identifying the biological substrate for light-cell interaction and their relations to cancer treatment. In this study, a near-infrared (NIR) laser is focused into the nucleus (nNIR) or cytoplasm (cNIR) of a single living cell by a high numerical aperture condenser to dissect the novel role of cell nucleus in mediating NIR effects on mitochondrial dynamics of A549 non-small cell lung cancer cells. Our analysis showed that nNIR, but not cNIR, triggered mitochondrial fission in 10 min. In contrast, the fission/fusion balance of mitochondria directly exposed to cNIR does not change. While the same phenomenon is also triggered by single molecular interactions between epidermal growth factor (EGF) and its receptor EGFR, pharmacological studies with cetuximab, PD153035, and caffeine suggest EGF signaling crosstalk to DNA damaging response to mediate rapid mitochondrial fission as a result of nNIR irradiation. These results suggest that nuclear DNA integrity is a novel biological target for cellular response to NIR. |
| first_indexed | 2024-03-09T22:20:51Z |
| format | Article |
| id | doaj.art-15fd76bdfa7b4152ba386149acd51a68 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T22:20:51Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-15fd76bdfa7b4152ba386149acd51a682023-11-23T19:14:18ZengMDPI AGCells2073-44092022-02-0111462410.3390/cells11040624Nucleus Near-Infrared (nNIR) Irradiation of Single A549 Cells Induces DNA Damage and Activates EGFR Leading to Mitochondrial FissionMomoh Gbetuwa0Long-Sheng Lu1Tsung-Jen Wang2Yin-Ju Chen3Jeng-Fong Chiou4Tai-Yuan Su5Tzu-Sen Yang6Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, TaiwanGraduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, TaiwanDepartment of Ophthalmology, Taipei Medical University Hospital, Taipei 110, TaiwanGraduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, TaiwanDepartment of Radiation Oncology, Taipei Medical University Hospital, Taipei Medical University, Taipei 110, TaiwanDepartment of Electrical Engineering, Yuan-Ze University, Chung-Li 32003, TaiwanInternational PhD Program in Biomedical Engineering, Taipei Medical University, Taipei 110, TaiwanThere has been great interest in identifying the biological substrate for light-cell interaction and their relations to cancer treatment. In this study, a near-infrared (NIR) laser is focused into the nucleus (nNIR) or cytoplasm (cNIR) of a single living cell by a high numerical aperture condenser to dissect the novel role of cell nucleus in mediating NIR effects on mitochondrial dynamics of A549 non-small cell lung cancer cells. Our analysis showed that nNIR, but not cNIR, triggered mitochondrial fission in 10 min. In contrast, the fission/fusion balance of mitochondria directly exposed to cNIR does not change. While the same phenomenon is also triggered by single molecular interactions between epidermal growth factor (EGF) and its receptor EGFR, pharmacological studies with cetuximab, PD153035, and caffeine suggest EGF signaling crosstalk to DNA damaging response to mediate rapid mitochondrial fission as a result of nNIR irradiation. These results suggest that nuclear DNA integrity is a novel biological target for cellular response to NIR.https://www.mdpi.com/2073-4409/11/4/624near infrared (NIR)epidermal growth factor receptor (EGFR)mitochondrial fragmentation count (MFC)mitochondrial dynamiccetuximabcaffeine |
| spellingShingle | Momoh Gbetuwa Long-Sheng Lu Tsung-Jen Wang Yin-Ju Chen Jeng-Fong Chiou Tai-Yuan Su Tzu-Sen Yang Nucleus Near-Infrared (nNIR) Irradiation of Single A549 Cells Induces DNA Damage and Activates EGFR Leading to Mitochondrial Fission Cells near infrared (NIR) epidermal growth factor receptor (EGFR) mitochondrial fragmentation count (MFC) mitochondrial dynamic cetuximab caffeine |
| title | Nucleus Near-Infrared (nNIR) Irradiation of Single A549 Cells Induces DNA Damage and Activates EGFR Leading to Mitochondrial Fission |
| title_full | Nucleus Near-Infrared (nNIR) Irradiation of Single A549 Cells Induces DNA Damage and Activates EGFR Leading to Mitochondrial Fission |
| title_fullStr | Nucleus Near-Infrared (nNIR) Irradiation of Single A549 Cells Induces DNA Damage and Activates EGFR Leading to Mitochondrial Fission |
| title_full_unstemmed | Nucleus Near-Infrared (nNIR) Irradiation of Single A549 Cells Induces DNA Damage and Activates EGFR Leading to Mitochondrial Fission |
| title_short | Nucleus Near-Infrared (nNIR) Irradiation of Single A549 Cells Induces DNA Damage and Activates EGFR Leading to Mitochondrial Fission |
| title_sort | nucleus near infrared nnir irradiation of single a549 cells induces dna damage and activates egfr leading to mitochondrial fission |
| topic | near infrared (NIR) epidermal growth factor receptor (EGFR) mitochondrial fragmentation count (MFC) mitochondrial dynamic cetuximab caffeine |
| url | https://www.mdpi.com/2073-4409/11/4/624 |
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