Comparative Serum Proteome Profiling of Canine Benign Prostatic Hyperplasia before and after Castration
BPH is the most prevalent prostatic condition in aging dogs. Nevertheless, clinical diagnosis and management remain inconsistent. This study employed in-solution digestion coupled with nano-liquid chromatography tandem mass spectrometry to assess serum proteome profiling of dogs with BPH and those d...
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MDPI AG
2023-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/24/3853 |
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| author | Sekkarin Ploypetch Grisnarong Wongbandue Sittiruk Roytrakul Narumon Phaonakrop Nawarus Prapaiwan |
| author_facet | Sekkarin Ploypetch Grisnarong Wongbandue Sittiruk Roytrakul Narumon Phaonakrop Nawarus Prapaiwan |
| author_sort | Sekkarin Ploypetch |
| collection | DOAJ |
| description | BPH is the most prevalent prostatic condition in aging dogs. Nevertheless, clinical diagnosis and management remain inconsistent. This study employed in-solution digestion coupled with nano-liquid chromatography tandem mass spectrometry to assess serum proteome profiling of dogs with BPH and those dogs after castration. Male dogs were divided into two groups; control and BPH groups. In the BPH group, each dog was evaluated at two time points: Day 0 (BF subgroup) and Day 30 after castration (AT subgroup). In the BF subgroup, three proteins were significantly upregulated and associated with dihydrotestosterone: solute carrier family 5 member 5, tyrosine-protein kinase, and FRAT regulator of WNT signaling pathway 1. Additionally, the overexpression of polymeric immunoglobulin receptors in the BF subgroup hints at its potential as a novel protein linked to the BPH development process. Conversely, alpha-1-B glycoprotein (A1BG) displayed significant downregulation in the BF subgroup, suggesting A1BG’s potential as a predictive protein for canine BPH. Finasteride was associated with increased proteins in the AT subgroup, including apolipoprotein C-I, apolipoprotein E, apolipoprotein A-II, TAO kinase 1, DnaJ homolog subfamily C member 16, PH domain and leucine-rich repeat protein phosphatase 1, neuregulin 1, and pseudopodium enriched atypical kinase 1. In conclusion, this pilot study highlighted alterations in various serum proteins in canine BPH, reflecting different pathological changes occurring in this condition. These proteins could be a source of potential non-invasive biomarkers for diagnosing this disease. |
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| format | Article |
| id | doaj.art-1602d44706b7405aa1216cd2b66d5d9f |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T21:03:13Z |
| publishDate | 2023-12-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-1602d44706b7405aa1216cd2b66d5d9f2023-12-22T13:47:37ZengMDPI AGAnimals2076-26152023-12-011324385310.3390/ani13243853Comparative Serum Proteome Profiling of Canine Benign Prostatic Hyperplasia before and after CastrationSekkarin Ploypetch0Grisnarong Wongbandue1Sittiruk Roytrakul2Narumon Phaonakrop3Nawarus Prapaiwan4Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, ThailandDepartment of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, ThailandFunctional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, ThailandFunctional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, ThailandDepartment of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, ThailandBPH is the most prevalent prostatic condition in aging dogs. Nevertheless, clinical diagnosis and management remain inconsistent. This study employed in-solution digestion coupled with nano-liquid chromatography tandem mass spectrometry to assess serum proteome profiling of dogs with BPH and those dogs after castration. Male dogs were divided into two groups; control and BPH groups. In the BPH group, each dog was evaluated at two time points: Day 0 (BF subgroup) and Day 30 after castration (AT subgroup). In the BF subgroup, three proteins were significantly upregulated and associated with dihydrotestosterone: solute carrier family 5 member 5, tyrosine-protein kinase, and FRAT regulator of WNT signaling pathway 1. Additionally, the overexpression of polymeric immunoglobulin receptors in the BF subgroup hints at its potential as a novel protein linked to the BPH development process. Conversely, alpha-1-B glycoprotein (A1BG) displayed significant downregulation in the BF subgroup, suggesting A1BG’s potential as a predictive protein for canine BPH. Finasteride was associated with increased proteins in the AT subgroup, including apolipoprotein C-I, apolipoprotein E, apolipoprotein A-II, TAO kinase 1, DnaJ homolog subfamily C member 16, PH domain and leucine-rich repeat protein phosphatase 1, neuregulin 1, and pseudopodium enriched atypical kinase 1. In conclusion, this pilot study highlighted alterations in various serum proteins in canine BPH, reflecting different pathological changes occurring in this condition. These proteins could be a source of potential non-invasive biomarkers for diagnosing this disease.https://www.mdpi.com/2076-2615/13/24/3853benign prostatic hyperplasiacastrationdogsproteomeserum proteins |
| spellingShingle | Sekkarin Ploypetch Grisnarong Wongbandue Sittiruk Roytrakul Narumon Phaonakrop Nawarus Prapaiwan Comparative Serum Proteome Profiling of Canine Benign Prostatic Hyperplasia before and after Castration Animals benign prostatic hyperplasia castration dogs proteome serum proteins |
| title | Comparative Serum Proteome Profiling of Canine Benign Prostatic Hyperplasia before and after Castration |
| title_full | Comparative Serum Proteome Profiling of Canine Benign Prostatic Hyperplasia before and after Castration |
| title_fullStr | Comparative Serum Proteome Profiling of Canine Benign Prostatic Hyperplasia before and after Castration |
| title_full_unstemmed | Comparative Serum Proteome Profiling of Canine Benign Prostatic Hyperplasia before and after Castration |
| title_short | Comparative Serum Proteome Profiling of Canine Benign Prostatic Hyperplasia before and after Castration |
| title_sort | comparative serum proteome profiling of canine benign prostatic hyperplasia before and after castration |
| topic | benign prostatic hyperplasia castration dogs proteome serum proteins |
| url | https://www.mdpi.com/2076-2615/13/24/3853 |
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