C-Type Natriuretic Peptide/Natriuretic Peptide Receptor 2 Is Involved in Cell Proliferation and Testosterone Production in Mouse Leydig Cells
Purpose: This study investigated the role of natriuretic peptide receptor 2 (NPR2) on cell proliferation and testosterone secre-tion in mouse Leydig cells.Materials and Methods: Mouse testis of different postnatal stages was isolated to detect the expression C-type natriuretic peptide (CNP) and its...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society for Sexual Medicine and Andrology
2019-05-01
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| Series: | The World Journal of Men's Health |
| Subjects: |
| _version_ | 1818305668808966144 |
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| author | Lei Yang Lanjie Lei Qihan Zhao Ying Gong Gaopeng Guan Shaoxin Huang |
| author_facet | Lei Yang Lanjie Lei Qihan Zhao Ying Gong Gaopeng Guan Shaoxin Huang |
| author_sort | Lei Yang |
| collection | DOAJ |
| description | Purpose: This study investigated the role of natriuretic peptide receptor 2 (NPR2) on cell proliferation and testosterone secre-tion in mouse Leydig cells.Materials and Methods: Mouse testis of different postnatal stages was isolated to detect the expression C-type natriuretic peptide (CNP) and its receptor NPR2 by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Leydig cells isolated from mouse testis were cultured and treated with shNPR2 lentiviruses or CNP. And then the cyclic guanosine mono-phosphate production, testosterone secretion, cell proliferation, cell cycle and cell apoptosis in mouse Leydig cells were ana-lyzed by ELISA, RT-qPCR, Cell Counting Kit-8, and flow cytometry. Moreover, the expression of NPR2, cell cycle, apoptosis proliferation and cell cycle related gene were detected by RT-qPCR and Western blot. Results: Knockdown of NPR2 by RNAi resulted in S phase cell cycle arrest, cell apoptosis, and decreased testosterone secre-tion in mouse Leydig cells.Conclusions: Our study provides more evidences to better understand the function of CNP/NPR2 pathway in male reproduc-tion, which may help us to treat male infertility. |
| first_indexed | 2024-12-13T06:30:15Z |
| format | Article |
| id | doaj.art-1604851177244100a121f96164a63880 |
| institution | Directory Open Access Journal |
| issn | 2287-4208 2287-4690 |
| language | English |
| last_indexed | 2024-12-13T06:30:15Z |
| publishDate | 2019-05-01 |
| publisher | Korean Society for Sexual Medicine and Andrology |
| record_format | Article |
| series | The World Journal of Men's Health |
| spelling | doaj.art-1604851177244100a121f96164a638802022-12-21T23:56:37ZengKorean Society for Sexual Medicine and AndrologyThe World Journal of Men's Health2287-42082287-46902019-05-0137218619810.5534/wjmh.180041C-Type Natriuretic Peptide/Natriuretic Peptide Receptor 2 Is Involved in Cell Proliferation and Testosterone Production in Mouse Leydig CellsLei Yang0Lanjie Lei1Qihan Zhao2Ying Gong3Gaopeng Guan4Shaoxin Huang5Jiujiang University, Jiujiang, ChinaJiujiang University, Jiujiang, ChinaJiujiang University, Jiujiang, ChinaJiujiang University, Jiujiang, ChinaJiujiang University, Jiujiang, ChinaJiujiang University, Jiujiang, ChinaPurpose: This study investigated the role of natriuretic peptide receptor 2 (NPR2) on cell proliferation and testosterone secre-tion in mouse Leydig cells.Materials and Methods: Mouse testis of different postnatal stages was isolated to detect the expression C-type natriuretic peptide (CNP) and its receptor NPR2 by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Leydig cells isolated from mouse testis were cultured and treated with shNPR2 lentiviruses or CNP. And then the cyclic guanosine mono-phosphate production, testosterone secretion, cell proliferation, cell cycle and cell apoptosis in mouse Leydig cells were ana-lyzed by ELISA, RT-qPCR, Cell Counting Kit-8, and flow cytometry. Moreover, the expression of NPR2, cell cycle, apoptosis proliferation and cell cycle related gene were detected by RT-qPCR and Western blot. Results: Knockdown of NPR2 by RNAi resulted in S phase cell cycle arrest, cell apoptosis, and decreased testosterone secre-tion in mouse Leydig cells.Conclusions: Our study provides more evidences to better understand the function of CNP/NPR2 pathway in male reproduc-tion, which may help us to treat male infertility.Germ cellsLeydig cellsTesticular diseasesTestosterone |
| spellingShingle | Lei Yang Lanjie Lei Qihan Zhao Ying Gong Gaopeng Guan Shaoxin Huang C-Type Natriuretic Peptide/Natriuretic Peptide Receptor 2 Is Involved in Cell Proliferation and Testosterone Production in Mouse Leydig Cells The World Journal of Men's Health Germ cells Leydig cells Testicular diseases Testosterone |
| title | C-Type Natriuretic Peptide/Natriuretic Peptide Receptor 2 Is Involved in Cell Proliferation and Testosterone Production in Mouse Leydig Cells |
| title_full | C-Type Natriuretic Peptide/Natriuretic Peptide Receptor 2 Is Involved in Cell Proliferation and Testosterone Production in Mouse Leydig Cells |
| title_fullStr | C-Type Natriuretic Peptide/Natriuretic Peptide Receptor 2 Is Involved in Cell Proliferation and Testosterone Production in Mouse Leydig Cells |
| title_full_unstemmed | C-Type Natriuretic Peptide/Natriuretic Peptide Receptor 2 Is Involved in Cell Proliferation and Testosterone Production in Mouse Leydig Cells |
| title_short | C-Type Natriuretic Peptide/Natriuretic Peptide Receptor 2 Is Involved in Cell Proliferation and Testosterone Production in Mouse Leydig Cells |
| title_sort | c type natriuretic peptide natriuretic peptide receptor 2 is involved in cell proliferation and testosterone production in mouse leydig cells |
| topic | Germ cells Leydig cells Testicular diseases Testosterone |
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