Detection of Cutaneous Leishmaniasis by PCR in Fasa district in 2012
Background & Objective: Leishmaniasis is a common disease between humans and animals caused by protozoa of the genus Leishmania spp. It is transmitted by the female species of Phlebotomus.The most common form of leishmaniasis is the cutaneous form seen as either dry (urban) or wet (Rural). Detec...
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| Format: | Article |
| Language: | fas |
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Fasa University of Medical Sciences
2013-09-01
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| Series: | Journal of Fasa University of Medical Sciences |
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| Online Access: | http://journal.fums.ac.ir/browse.php?a_code=A-10-201-1&slc_lang=en&sid=1 |
| _version_ | 1818833379841277952 |
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| author | Mehdi Sharafi Babak Pezeshki Abdollah Reisi Mohsen Kalantari Mohammad Mehdi Naghizadeh Soulmaz Dast Manesh |
| author_facet | Mehdi Sharafi Babak Pezeshki Abdollah Reisi Mohsen Kalantari Mohammad Mehdi Naghizadeh Soulmaz Dast Manesh |
| author_sort | Mehdi Sharafi |
| collection | DOAJ |
| description | Background & Objective: Leishmaniasis is a common disease between humans and animals caused by protozoa of the genus Leishmania spp. It is transmitted by the female species of Phlebotomus.The most common form of leishmaniasis is the cutaneous form seen as either dry (urban) or wet (Rural). Detection of cutaneous leishmaniasis species is not reliable by microscopy. Among the methods, PCR is useful for this purpose. In this study, we aimed to investigate the cutaneous leishmaniasis species by PCR method in Fasa district.
Material & Methods: Slit biopsies were collected from 138 suspected cases of cutaneous leishmaniasis who were presented, consecutively, in Fasa district from April 2011 to February 2012. After the microscopy, the entire smear was then scraped off the slide surface for DNA extraction and PCR assay.
Results: All 138 investigated smears were reported positive by microscopy and nested PCR assay. In PCR, One of the smears had the 750-bp band, indicative of L. tropica and the rest had the 560-bp band indicative of L. major.
Conclusion: In conclusion, the cutaneous leishmaniasis found in Fasa district is predominantly Rural Cutaneous Leishmaniasis. The PCR-based assay used in the present study appears to be a suitable and powerful tool for the characterization of Leishmanial species. |
| first_indexed | 2024-12-19T02:18:00Z |
| format | Article |
| id | doaj.art-1606f29bb16548a9a817edc8c41ed75c |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2228-7329 |
| language | fas |
| last_indexed | 2024-12-19T02:18:00Z |
| publishDate | 2013-09-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Fasa University of Medical Sciences |
| spelling | doaj.art-1606f29bb16548a9a817edc8c41ed75c2022-12-21T20:40:21ZfasFasa University of Medical SciencesJournal of Fasa University of Medical Sciences2228-51052228-73292013-09-0133266270Detection of Cutaneous Leishmaniasis by PCR in Fasa district in 2012Mehdi Sharafi0Babak Pezeshki1Abdollah Reisi2Mohsen Kalantari3Mohammad Mehdi Naghizadeh4Soulmaz Dast Manesh5 Fasa University of Medical Sciences, Fasa, Iran. Fasa University of Medical Sciences, Fasa, Iran. Fasa University of Medical Sciences, Fasa, Iran. Mamasani Paramedical School, Shiraz University of Medical Sciences, Shiraz, Iran. Fasa University of Medical Sciences, Fasa, Iran. Hormozgan University of Medical Sciences, Iran. Background & Objective: Leishmaniasis is a common disease between humans and animals caused by protozoa of the genus Leishmania spp. It is transmitted by the female species of Phlebotomus.The most common form of leishmaniasis is the cutaneous form seen as either dry (urban) or wet (Rural). Detection of cutaneous leishmaniasis species is not reliable by microscopy. Among the methods, PCR is useful for this purpose. In this study, we aimed to investigate the cutaneous leishmaniasis species by PCR method in Fasa district. Material & Methods: Slit biopsies were collected from 138 suspected cases of cutaneous leishmaniasis who were presented, consecutively, in Fasa district from April 2011 to February 2012. After the microscopy, the entire smear was then scraped off the slide surface for DNA extraction and PCR assay. Results: All 138 investigated smears were reported positive by microscopy and nested PCR assay. In PCR, One of the smears had the 750-bp band, indicative of L. tropica and the rest had the 560-bp band indicative of L. major. Conclusion: In conclusion, the cutaneous leishmaniasis found in Fasa district is predominantly Rural Cutaneous Leishmaniasis. The PCR-based assay used in the present study appears to be a suitable and powerful tool for the characterization of Leishmanial species.http://journal.fums.ac.ir/browse.php?a_code=A-10-201-1&slc_lang=en&sid=1Cutaneous Leishmaniasis detection Iran. |
| spellingShingle | Mehdi Sharafi Babak Pezeshki Abdollah Reisi Mohsen Kalantari Mohammad Mehdi Naghizadeh Soulmaz Dast Manesh Detection of Cutaneous Leishmaniasis by PCR in Fasa district in 2012 Journal of Fasa University of Medical Sciences Cutaneous Leishmaniasis detection Iran. |
| title | Detection of Cutaneous Leishmaniasis by PCR in Fasa district in 2012 |
| title_full | Detection of Cutaneous Leishmaniasis by PCR in Fasa district in 2012 |
| title_fullStr | Detection of Cutaneous Leishmaniasis by PCR in Fasa district in 2012 |
| title_full_unstemmed | Detection of Cutaneous Leishmaniasis by PCR in Fasa district in 2012 |
| title_short | Detection of Cutaneous Leishmaniasis by PCR in Fasa district in 2012 |
| title_sort | detection of cutaneous leishmaniasis by pcr in fasa district in 2012 |
| topic | Cutaneous Leishmaniasis detection Iran. |
| url | http://journal.fums.ac.ir/browse.php?a_code=A-10-201-1&slc_lang=en&sid=1 |
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