Theaflavin-3,3′-digallate inhibits ovarian cancer stem cells via suppressing Wnt/β-Catenin signaling pathway
Recent evidence indicates that ovarian cancer stem cells (CSCs) are responsible for ovarian cancer recurrence and drug resistance, resulting in the low long-term survival rate of patients with advanced ovarian cancer. We aimed to study the inhibitory effect of theaflavin-3,3′-digallate (TF3), a blac...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-11-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464618304961 |
| _version_ | 1819292697665470464 |
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| author | Haibo Pan Eunhye Kim Gary O. Rankin Yon Rojanasakul Youying Tu Yi Charlie Chen |
| author_facet | Haibo Pan Eunhye Kim Gary O. Rankin Yon Rojanasakul Youying Tu Yi Charlie Chen |
| author_sort | Haibo Pan |
| collection | DOAJ |
| description | Recent evidence indicates that ovarian cancer stem cells (CSCs) are responsible for ovarian cancer recurrence and drug resistance, resulting in the low long-term survival rate of patients with advanced ovarian cancer. We aimed to study the inhibitory effect of theaflavin-3,3′-digallate (TF3), a black tea polyphenol on ovarian CSCs. Here, we showed that TF3 inhibited the proliferation of A2780/CP70 and OVCAR3 tumorshpere cells by suppressing their cell viability and colony formation capacity. TF3 inhibited the tumorsphere formation capacity of A2780/CP70 and OVCAR3 CSCs in serum-free and non-adherent conditions. TF3 inhibited A2780/CP70 and OVCAR3 CSCs isolated from tumorspheres by decreasing their cell viability and upregulating the protein expression of caspase-3 and -7 in the cells. We also revealed that TF3 inhibited ovarian CSCs through Wnt/β-catenin signaling pathway. Our results suggested that TF3 could inhibit ovarian CSCs and might be a potential agent for eradicating ovarian cancer. |
| first_indexed | 2024-12-24T03:58:39Z |
| format | Article |
| id | doaj.art-160a15be00e548718e3334e6f77e96bc |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T03:58:39Z |
| publishDate | 2018-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-160a15be00e548718e3334e6f77e96bc2022-12-21T17:16:22ZengElsevierJournal of Functional Foods1756-46462018-11-015017Theaflavin-3,3′-digallate inhibits ovarian cancer stem cells via suppressing Wnt/β-Catenin signaling pathwayHaibo Pan0Eunhye Kim1Gary O. Rankin2Yon Rojanasakul3Youying Tu4Yi Charlie Chen5Department of Tea Science, Zhejiang University, Hangzhou, Zhejiang, PR China; College of Science, Technology and Mathematics, Alderson Broaddus University, Philippi, WV, USADepartment of Tea Science, Zhejiang University, Hangzhou, Zhejiang, PR ChinaDepartment of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV, USADepartment of Pharmaceutical Sciences, West Virginia University, Morgantown, WV, USADepartment of Tea Science, Zhejiang University, Hangzhou, Zhejiang, PR China; Corresponding authors at: College of Science, Technology and Mathematics, Alderson Broaddus University, 101 College Hill Drive, Philippi, WV 26416, USA. (Y.C. Chen). Department of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China (Y. Tu).College of Science, Technology and Mathematics, Alderson Broaddus University, Philippi, WV, USA; Corresponding authors at: College of Science, Technology and Mathematics, Alderson Broaddus University, 101 College Hill Drive, Philippi, WV 26416, USA. (Y.C. Chen). Department of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China (Y. Tu).Recent evidence indicates that ovarian cancer stem cells (CSCs) are responsible for ovarian cancer recurrence and drug resistance, resulting in the low long-term survival rate of patients with advanced ovarian cancer. We aimed to study the inhibitory effect of theaflavin-3,3′-digallate (TF3), a black tea polyphenol on ovarian CSCs. Here, we showed that TF3 inhibited the proliferation of A2780/CP70 and OVCAR3 tumorshpere cells by suppressing their cell viability and colony formation capacity. TF3 inhibited the tumorsphere formation capacity of A2780/CP70 and OVCAR3 CSCs in serum-free and non-adherent conditions. TF3 inhibited A2780/CP70 and OVCAR3 CSCs isolated from tumorspheres by decreasing their cell viability and upregulating the protein expression of caspase-3 and -7 in the cells. We also revealed that TF3 inhibited ovarian CSCs through Wnt/β-catenin signaling pathway. Our results suggested that TF3 could inhibit ovarian CSCs and might be a potential agent for eradicating ovarian cancer.http://www.sciencedirect.com/science/article/pii/S1756464618304961Theaflavin-33′-digallateOvarian cancer stem cellAnti-proliferationALDHβ-Catenin |
| spellingShingle | Haibo Pan Eunhye Kim Gary O. Rankin Yon Rojanasakul Youying Tu Yi Charlie Chen Theaflavin-3,3′-digallate inhibits ovarian cancer stem cells via suppressing Wnt/β-Catenin signaling pathway Journal of Functional Foods Theaflavin-3 3′-digallate Ovarian cancer stem cell Anti-proliferation ALDH β-Catenin |
| title | Theaflavin-3,3′-digallate inhibits ovarian cancer stem cells via suppressing Wnt/β-Catenin signaling pathway |
| title_full | Theaflavin-3,3′-digallate inhibits ovarian cancer stem cells via suppressing Wnt/β-Catenin signaling pathway |
| title_fullStr | Theaflavin-3,3′-digallate inhibits ovarian cancer stem cells via suppressing Wnt/β-Catenin signaling pathway |
| title_full_unstemmed | Theaflavin-3,3′-digallate inhibits ovarian cancer stem cells via suppressing Wnt/β-Catenin signaling pathway |
| title_short | Theaflavin-3,3′-digallate inhibits ovarian cancer stem cells via suppressing Wnt/β-Catenin signaling pathway |
| title_sort | theaflavin 3 3 digallate inhibits ovarian cancer stem cells via suppressing wnt β catenin signaling pathway |
| topic | Theaflavin-3 3′-digallate Ovarian cancer stem cell Anti-proliferation ALDH β-Catenin |
| url | http://www.sciencedirect.com/science/article/pii/S1756464618304961 |
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