Association of PRDM16 rs12409277 and CtBP2 rs1561589 gene polymorphisms with lipid profile of adolescents
Introduction Positive regulatory domain containing 16 (PRDM16) protein represents the key regulator of brown adipose tissue (BAT) development. It induces brown fat phenotype and represses white adipose tissue specific genes through the association with C-terminal binding co-repressor proteins (CtBP1...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2021-01-01
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| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | https://www.archivesofmedicalscience.com/Association-of-PRDM16-rs12409277-and-CtBP2-rs1561589-gene-polymorphisms-with-lipid,113174,0,2.html |
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| author | Nela Maksimovic Vanja Vidovic Tatjana Damnjanovic Biljana Jekic Nada Majkic Singh Slavko Simeunovic Dara Savic Bozovic Stojko Vidovic Ivana Novakovic |
| author_facet | Nela Maksimovic Vanja Vidovic Tatjana Damnjanovic Biljana Jekic Nada Majkic Singh Slavko Simeunovic Dara Savic Bozovic Stojko Vidovic Ivana Novakovic |
| author_sort | Nela Maksimovic |
| collection | DOAJ |
| description | Introduction
Positive regulatory domain containing 16 (PRDM16) protein represents the key regulator of brown adipose tissue (BAT) development. It induces brown fat phenotype and represses white adipose tissue specific genes through the association with C-terminal binding co-repressor proteins (CtBP1 and CtBP2). In healthy adults presence of BAT has been associated with lower glucose, total cholesterol and low-density lipoprotein (LDL) cholesterol levels. Our aim was to analyze the association of PRDM16 gene (rs12409277) and CtBP2 gene (rs1561589) polymorphisms with body mass index (BMI), fasting glucose level and lipid profile of adolescents.
Material and methods
Our study included 295 healthy school children, 145 boys (49.2%) and 150 girls (50.8%), 15 years of age. Genotypes for the selected polymorphisms were detected by the real-time PCR method. Age, gender, height, weight, lipid profile (total cholesterol, high-density lipoprotein (HDL) cholesterol, LDL cholesterol, triglycerides) and fasting glucose levels were recorded.
Results
We did not find a statistically significant association of rs12409277 and rs1561589 polymorphisms with BMI, fasting glucose and lipid profile of adolescents. We further analyzed the combined effect of the two SNPs and the statistical analysis showed that carriers of CT genotype of rs12409277 polymorphism and GG genotype of rs1561589 polymorphism had significantly lower total cholesterol (p = 0.001) and LDL cholesterol (p = 0.008) levels compared to all other groups of genotypes.
Conclusions
Our study suggests that rs12409277 and rs1561589 polymorphism might have an influence on total and LDL cholesterol levels in adolescents. Larger studies should be performed in order to confirm our results. |
| first_indexed | 2024-03-09T10:47:05Z |
| format | Article |
| id | doaj.art-160cb88bd27740d6b110cd35dd79238c |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-03-09T10:47:05Z |
| publishDate | 2021-01-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-160cb88bd27740d6b110cd35dd79238c2023-12-01T09:51:47ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512021-01-0119359359910.5114/aoms/113174113174Association of PRDM16 rs12409277 and CtBP2 rs1561589 gene polymorphisms with lipid profile of adolescentsNela Maksimovic0Vanja Vidovic1Tatjana Damnjanovic2Biljana Jekic3Nada Majkic Singh4Slavko Simeunovic5Dara Savic Bozovic6Stojko Vidovic7Ivana Novakovic8Institute of Human Genetics, Faculty of Medicine, University of BelgradeFaculty of Medicine, University of Banja Luka, Banja Luka, Bosnia and HerzegovinaInstitute of Human Genetics, Faculty of Medicine, University of BelgradeInstitute of Human Genetics, Faculty of Medicine, University of BelgradeSociety of Medical Biochemists, Belgrade, SerbiaUniversity Children’s Hospital, Belgrade, SerbiaUzice General Hospital, Uzice, SerbiaFaculty of Medicine, University of Banja Luka, Banja Luka, Bosnia and HerzegovinaInstitute of Human Genetics, Faculty of Medicine, University of BelgradeIntroduction Positive regulatory domain containing 16 (PRDM16) protein represents the key regulator of brown adipose tissue (BAT) development. It induces brown fat phenotype and represses white adipose tissue specific genes through the association with C-terminal binding co-repressor proteins (CtBP1 and CtBP2). In healthy adults presence of BAT has been associated with lower glucose, total cholesterol and low-density lipoprotein (LDL) cholesterol levels. Our aim was to analyze the association of PRDM16 gene (rs12409277) and CtBP2 gene (rs1561589) polymorphisms with body mass index (BMI), fasting glucose level and lipid profile of adolescents. Material and methods Our study included 295 healthy school children, 145 boys (49.2%) and 150 girls (50.8%), 15 years of age. Genotypes for the selected polymorphisms were detected by the real-time PCR method. Age, gender, height, weight, lipid profile (total cholesterol, high-density lipoprotein (HDL) cholesterol, LDL cholesterol, triglycerides) and fasting glucose levels were recorded. Results We did not find a statistically significant association of rs12409277 and rs1561589 polymorphisms with BMI, fasting glucose and lipid profile of adolescents. We further analyzed the combined effect of the two SNPs and the statistical analysis showed that carriers of CT genotype of rs12409277 polymorphism and GG genotype of rs1561589 polymorphism had significantly lower total cholesterol (p = 0.001) and LDL cholesterol (p = 0.008) levels compared to all other groups of genotypes. Conclusions Our study suggests that rs12409277 and rs1561589 polymorphism might have an influence on total and LDL cholesterol levels in adolescents. Larger studies should be performed in order to confirm our results.https://www.archivesofmedicalscience.com/Association-of-PRDM16-rs12409277-and-CtBP2-rs1561589-gene-polymorphisms-with-lipid,113174,0,2.htmlpolymorphismsbody mass indexlipidsadolescentsbrown adipose tissue |
| spellingShingle | Nela Maksimovic Vanja Vidovic Tatjana Damnjanovic Biljana Jekic Nada Majkic Singh Slavko Simeunovic Dara Savic Bozovic Stojko Vidovic Ivana Novakovic Association of PRDM16 rs12409277 and CtBP2 rs1561589 gene polymorphisms with lipid profile of adolescents Archives of Medical Science polymorphisms body mass index lipids adolescents brown adipose tissue |
| title | Association of PRDM16 rs12409277 and CtBP2 rs1561589 gene polymorphisms with lipid profile of adolescents |
| title_full | Association of PRDM16 rs12409277 and CtBP2 rs1561589 gene polymorphisms with lipid profile of adolescents |
| title_fullStr | Association of PRDM16 rs12409277 and CtBP2 rs1561589 gene polymorphisms with lipid profile of adolescents |
| title_full_unstemmed | Association of PRDM16 rs12409277 and CtBP2 rs1561589 gene polymorphisms with lipid profile of adolescents |
| title_short | Association of PRDM16 rs12409277 and CtBP2 rs1561589 gene polymorphisms with lipid profile of adolescents |
| title_sort | association of prdm16 rs12409277 and ctbp2 rs1561589 gene polymorphisms with lipid profile of adolescents |
| topic | polymorphisms body mass index lipids adolescents brown adipose tissue |
| url | https://www.archivesofmedicalscience.com/Association-of-PRDM16-rs12409277-and-CtBP2-rs1561589-gene-polymorphisms-with-lipid,113174,0,2.html |
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