The V617I Substitution in Avian Coronavirus IBV Spike Protein Plays a Crucial Role in Adaptation to Primary Chicken Kidney Cells
The naturally isolated avian coronavirus infectious bronchitis virus (IBV) generally cannot replicate in chicken kidney (CK) cells. To explore the molecular mechanism of IBV adapting to CK cells, a series of recombinant viruses were constructed by chimerizing the S genes of CK cell-adapted strain H1...
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| Format: | Article |
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.604335/full |
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| author | Yi Jiang Yi Jiang Yi Jiang Mingyan Gao Mingyan Gao Xu Cheng Xu Cheng Yan Yu Yan Yu Xinyue Shen Xinyue Shen Xinyue Shen Jianmei Li Jianmei Li Sheng Zhou Sheng Zhou |
| author_facet | Yi Jiang Yi Jiang Yi Jiang Mingyan Gao Mingyan Gao Xu Cheng Xu Cheng Yan Yu Yan Yu Xinyue Shen Xinyue Shen Xinyue Shen Jianmei Li Jianmei Li Sheng Zhou Sheng Zhou |
| author_sort | Yi Jiang |
| collection | DOAJ |
| description | The naturally isolated avian coronavirus infectious bronchitis virus (IBV) generally cannot replicate in chicken kidney (CK) cells. To explore the molecular mechanism of IBV adapting to CK cells, a series of recombinant viruses were constructed by chimerizing the S genes of CK cell-adapted strain H120 and non-adapted strain IBYZ. The results showed that the S2 subunit determines the difference in cell tropism of the two strains. After comparing the amino acid sequences of S protein of CK cell-adapted strain YZ120, with its parental strain IBYZ, three amino acid substitutions, A138V, L581F, and V617I, were identified. Using YZ120 as the backbone, one or more of the above-mentioned substitutions were eliminated to verify the correlation between these sites and CK cell tropism. The results showed that the CK cell tropism of the YZ120 strain depends on the V617I substitution, the change of L581F promoted the adaptation in CK cells, and the change at 138 position was not directly related to the CK cell tropism. Further validation experiments also showed that V617I had a decisive role in the adaptation of IBV to CK cells, but other areas of the virus genome also affected the replication efficiency of the virus in CK cells. |
| first_indexed | 2024-12-14T03:15:49Z |
| format | Article |
| id | doaj.art-160e8b0ca4b9432398df42bb85d92322 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T03:15:49Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-160e8b0ca4b9432398df42bb85d923222022-12-21T23:19:08ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-12-011110.3389/fmicb.2020.604335604335The V617I Substitution in Avian Coronavirus IBV Spike Protein Plays a Crucial Role in Adaptation to Primary Chicken Kidney CellsYi Jiang0Yi Jiang1Yi Jiang2Mingyan Gao3Mingyan Gao4Xu Cheng5Xu Cheng6Yan Yu7Yan Yu8Xinyue Shen9Xinyue Shen10Xinyue Shen11Jianmei Li12Jianmei Li13Sheng Zhou14Sheng Zhou15Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, ChinaJiangsu Institute of Poultry Science (CAAS), Yangzhou, ChinaLaboratory of Animal Infectious Disease, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaPoultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, ChinaJiangsu Institute of Poultry Science (CAAS), Yangzhou, ChinaPoultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, ChinaJiangsu Institute of Poultry Science (CAAS), Yangzhou, ChinaPoultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, ChinaJiangsu Institute of Poultry Science (CAAS), Yangzhou, ChinaPoultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, ChinaJiangsu Institute of Poultry Science (CAAS), Yangzhou, ChinaLaboratory of Animal Infectious Disease, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaPoultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, ChinaJiangsu Institute of Poultry Science (CAAS), Yangzhou, ChinaPoultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, ChinaJiangsu Institute of Poultry Science (CAAS), Yangzhou, ChinaThe naturally isolated avian coronavirus infectious bronchitis virus (IBV) generally cannot replicate in chicken kidney (CK) cells. To explore the molecular mechanism of IBV adapting to CK cells, a series of recombinant viruses were constructed by chimerizing the S genes of CK cell-adapted strain H120 and non-adapted strain IBYZ. The results showed that the S2 subunit determines the difference in cell tropism of the two strains. After comparing the amino acid sequences of S protein of CK cell-adapted strain YZ120, with its parental strain IBYZ, three amino acid substitutions, A138V, L581F, and V617I, were identified. Using YZ120 as the backbone, one or more of the above-mentioned substitutions were eliminated to verify the correlation between these sites and CK cell tropism. The results showed that the CK cell tropism of the YZ120 strain depends on the V617I substitution, the change of L581F promoted the adaptation in CK cells, and the change at 138 position was not directly related to the CK cell tropism. Further validation experiments also showed that V617I had a decisive role in the adaptation of IBV to CK cells, but other areas of the virus genome also affected the replication efficiency of the virus in CK cells.https://www.frontiersin.org/articles/10.3389/fmicb.2020.604335/fullinfectious bronchitis virusCK cellstropismamino acid mutationspike |
| spellingShingle | Yi Jiang Yi Jiang Yi Jiang Mingyan Gao Mingyan Gao Xu Cheng Xu Cheng Yan Yu Yan Yu Xinyue Shen Xinyue Shen Xinyue Shen Jianmei Li Jianmei Li Sheng Zhou Sheng Zhou The V617I Substitution in Avian Coronavirus IBV Spike Protein Plays a Crucial Role in Adaptation to Primary Chicken Kidney Cells Frontiers in Microbiology infectious bronchitis virus CK cells tropism amino acid mutation spike |
| title | The V617I Substitution in Avian Coronavirus IBV Spike Protein Plays a Crucial Role in Adaptation to Primary Chicken Kidney Cells |
| title_full | The V617I Substitution in Avian Coronavirus IBV Spike Protein Plays a Crucial Role in Adaptation to Primary Chicken Kidney Cells |
| title_fullStr | The V617I Substitution in Avian Coronavirus IBV Spike Protein Plays a Crucial Role in Adaptation to Primary Chicken Kidney Cells |
| title_full_unstemmed | The V617I Substitution in Avian Coronavirus IBV Spike Protein Plays a Crucial Role in Adaptation to Primary Chicken Kidney Cells |
| title_short | The V617I Substitution in Avian Coronavirus IBV Spike Protein Plays a Crucial Role in Adaptation to Primary Chicken Kidney Cells |
| title_sort | v617i substitution in avian coronavirus ibv spike protein plays a crucial role in adaptation to primary chicken kidney cells |
| topic | infectious bronchitis virus CK cells tropism amino acid mutation spike |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.604335/full |
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