Potent Anti-Inflammatory Effects of a Helix-to-Helix Peptide against <i>Pseudomonas aeruginosa</i> Endotoxin-Mediated Sepsis
Although considerable scientific research data is available for sepsis and cytokine storm syndrome, there is a need to develop new treatments or drugs for sepsis management. Antimicrobial peptides (AMPs) possess anti-bacterial and anti-inflammatory activity, neutralizing toxins such as lipopolysacch...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/11/1675 |
| _version_ | 1797466069369094144 |
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| author | Hyosuk Son Seong-Cheol Park Young-Min Kim Jong-Kook Lee Soyoung Park Taeuk Guk A-Mi Yoon Hye Song Lim Mi-Kyeong Jang Jung Ro Lee |
| author_facet | Hyosuk Son Seong-Cheol Park Young-Min Kim Jong-Kook Lee Soyoung Park Taeuk Guk A-Mi Yoon Hye Song Lim Mi-Kyeong Jang Jung Ro Lee |
| author_sort | Hyosuk Son |
| collection | DOAJ |
| description | Although considerable scientific research data is available for sepsis and cytokine storm syndrome, there is a need to develop new treatments or drugs for sepsis management. Antimicrobial peptides (AMPs) possess anti-bacterial and anti-inflammatory activity, neutralizing toxins such as lipopolysaccharides (LPS, endotoxin). Most AMPs have been designed as a substitute for conventional antibiotics, which kill drug-resistant pathogens. The present study aimed to determine the anti-inflammatory potential of 10 designed XIW (X: lysine, arginine, or glutamic acid) α-helical peptides in macrophages and a mouse model in the presence of LPS. Among them, WIKE-14, a peptide with a helix-to-helix structure, having the 12th amino acid substituted with glutamic acid, suppressed pro-inflammatory cytokines in RAW 264.7 macrophages. This reaction was mediated by the inhibition of the binding between LPS and macrophages. In addition, the WIKE-14 peptide exhibited a potent anti-inflammatory activity in mice ears and lungs inflamed using LPS. Thus, our results may provide useful insights for the development of anti-sepsis agents via the sequence and structure information of the WIKE-14 peptide. |
| first_indexed | 2024-03-09T18:30:56Z |
| format | Article |
| id | doaj.art-1610b8d358ce4a338c21966e6c226c46 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T18:30:56Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-1610b8d358ce4a338c21966e6c226c462023-11-24T07:31:40ZengMDPI AGAntibiotics2079-63822022-11-011111167510.3390/antibiotics11111675Potent Anti-Inflammatory Effects of a Helix-to-Helix Peptide against <i>Pseudomonas aeruginosa</i> Endotoxin-Mediated SepsisHyosuk Son0Seong-Cheol Park1Young-Min Kim2Jong-Kook Lee3Soyoung Park4Taeuk Guk5A-Mi Yoon6Hye Song Lim7Mi-Kyeong Jang8Jung Ro Lee9Department of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaLMO Team, National Institute of Ecology (NIE), Seocheon 33657, Republic of KoreaLMO Team, National Institute of Ecology (NIE), Seocheon 33657, Republic of KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaLMO Team, National Institute of Ecology (NIE), Seocheon 33657, Republic of KoreaAlthough considerable scientific research data is available for sepsis and cytokine storm syndrome, there is a need to develop new treatments or drugs for sepsis management. Antimicrobial peptides (AMPs) possess anti-bacterial and anti-inflammatory activity, neutralizing toxins such as lipopolysaccharides (LPS, endotoxin). Most AMPs have been designed as a substitute for conventional antibiotics, which kill drug-resistant pathogens. The present study aimed to determine the anti-inflammatory potential of 10 designed XIW (X: lysine, arginine, or glutamic acid) α-helical peptides in macrophages and a mouse model in the presence of LPS. Among them, WIKE-14, a peptide with a helix-to-helix structure, having the 12th amino acid substituted with glutamic acid, suppressed pro-inflammatory cytokines in RAW 264.7 macrophages. This reaction was mediated by the inhibition of the binding between LPS and macrophages. In addition, the WIKE-14 peptide exhibited a potent anti-inflammatory activity in mice ears and lungs inflamed using LPS. Thus, our results may provide useful insights for the development of anti-sepsis agents via the sequence and structure information of the WIKE-14 peptide.https://www.mdpi.com/2079-6382/11/11/1675antimicrobial peptideseptic shocklipopolysaccharideanti-inflammatory activitycytokines |
| spellingShingle | Hyosuk Son Seong-Cheol Park Young-Min Kim Jong-Kook Lee Soyoung Park Taeuk Guk A-Mi Yoon Hye Song Lim Mi-Kyeong Jang Jung Ro Lee Potent Anti-Inflammatory Effects of a Helix-to-Helix Peptide against <i>Pseudomonas aeruginosa</i> Endotoxin-Mediated Sepsis Antibiotics antimicrobial peptide septic shock lipopolysaccharide anti-inflammatory activity cytokines |
| title | Potent Anti-Inflammatory Effects of a Helix-to-Helix Peptide against <i>Pseudomonas aeruginosa</i> Endotoxin-Mediated Sepsis |
| title_full | Potent Anti-Inflammatory Effects of a Helix-to-Helix Peptide against <i>Pseudomonas aeruginosa</i> Endotoxin-Mediated Sepsis |
| title_fullStr | Potent Anti-Inflammatory Effects of a Helix-to-Helix Peptide against <i>Pseudomonas aeruginosa</i> Endotoxin-Mediated Sepsis |
| title_full_unstemmed | Potent Anti-Inflammatory Effects of a Helix-to-Helix Peptide against <i>Pseudomonas aeruginosa</i> Endotoxin-Mediated Sepsis |
| title_short | Potent Anti-Inflammatory Effects of a Helix-to-Helix Peptide against <i>Pseudomonas aeruginosa</i> Endotoxin-Mediated Sepsis |
| title_sort | potent anti inflammatory effects of a helix to helix peptide against i pseudomonas aeruginosa i endotoxin mediated sepsis |
| topic | antimicrobial peptide septic shock lipopolysaccharide anti-inflammatory activity cytokines |
| url | https://www.mdpi.com/2079-6382/11/11/1675 |
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