Revisiting bistability in the lysis/lysogeny circuit of bacteriophage lambda.
The lysis/lysogeny switch of bacteriophage lambda serves as a paradigm for binary cell fate decision, long-term maintenance of cellular state and stimulus-triggered switching between states. In the literature, the system is often referred to as "bistable." However, it remains unclear wheth...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4070997?pdf=render |
| _version_ | 1818584490743693312 |
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| author | Michael Bednarz Jennifer A Halliday Christophe Herman Ido Golding |
| author_facet | Michael Bednarz Jennifer A Halliday Christophe Herman Ido Golding |
| author_sort | Michael Bednarz |
| collection | DOAJ |
| description | The lysis/lysogeny switch of bacteriophage lambda serves as a paradigm for binary cell fate decision, long-term maintenance of cellular state and stimulus-triggered switching between states. In the literature, the system is often referred to as "bistable." However, it remains unclear whether this term provides an accurate description or is instead a misnomer. Here we address this question directly. We first quantify transcriptional regulation governing lysogenic maintenance using a single-cell fluorescence reporter. We then use the single-cell data to derive a stochastic theoretical model for the underlying regulatory network. We use the model to predict the steady states of the system and then validate these predictions experimentally. Specifically, a regime of bistability, and the resulting hysteretic behavior, are observed. Beyond the steady states, the theoretical model successfully predicts the kinetics of switching from lysogeny to lysis. Our results show how the physics-inspired concept of bistability can be reliably used to describe cellular phenotype, and how an experimentally-calibrated theoretical model can have accurate predictive power for cell-state switching. |
| first_indexed | 2024-12-16T08:22:01Z |
| format | Article |
| id | doaj.art-1612d6f456a04b4283717a2bec5ee178 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T08:22:01Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1612d6f456a04b4283717a2bec5ee1782022-12-21T22:38:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e10087610.1371/journal.pone.0100876Revisiting bistability in the lysis/lysogeny circuit of bacteriophage lambda.Michael BednarzJennifer A HallidayChristophe HermanIdo GoldingThe lysis/lysogeny switch of bacteriophage lambda serves as a paradigm for binary cell fate decision, long-term maintenance of cellular state and stimulus-triggered switching between states. In the literature, the system is often referred to as "bistable." However, it remains unclear whether this term provides an accurate description or is instead a misnomer. Here we address this question directly. We first quantify transcriptional regulation governing lysogenic maintenance using a single-cell fluorescence reporter. We then use the single-cell data to derive a stochastic theoretical model for the underlying regulatory network. We use the model to predict the steady states of the system and then validate these predictions experimentally. Specifically, a regime of bistability, and the resulting hysteretic behavior, are observed. Beyond the steady states, the theoretical model successfully predicts the kinetics of switching from lysogeny to lysis. Our results show how the physics-inspired concept of bistability can be reliably used to describe cellular phenotype, and how an experimentally-calibrated theoretical model can have accurate predictive power for cell-state switching.http://europepmc.org/articles/PMC4070997?pdf=render |
| spellingShingle | Michael Bednarz Jennifer A Halliday Christophe Herman Ido Golding Revisiting bistability in the lysis/lysogeny circuit of bacteriophage lambda. PLoS ONE |
| title | Revisiting bistability in the lysis/lysogeny circuit of bacteriophage lambda. |
| title_full | Revisiting bistability in the lysis/lysogeny circuit of bacteriophage lambda. |
| title_fullStr | Revisiting bistability in the lysis/lysogeny circuit of bacteriophage lambda. |
| title_full_unstemmed | Revisiting bistability in the lysis/lysogeny circuit of bacteriophage lambda. |
| title_short | Revisiting bistability in the lysis/lysogeny circuit of bacteriophage lambda. |
| title_sort | revisiting bistability in the lysis lysogeny circuit of bacteriophage lambda |
| url | http://europepmc.org/articles/PMC4070997?pdf=render |
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