Investigation of Growth Factor and Tenocyte Proliferation Induced by Platelet Rich Plasma (PRP) in a 3-Chamber Co-Culture Device
The platelet-rich plasma (PRP) has become an attractive topic for soft tissue healing therapy recently. While some clinical reports revealed the effective treatments for knee osteoarthritis, lateral epicondylitis, and rotator cuff tears, other case studies showed that there was no statistically sign...
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MDPI AG
2018-09-01
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Online Access: | http://www.mdpi.com/2072-666X/9/9/446 |
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author | Chih-Hao Chiu Rei Higashikawa Wen-Ling Yeh Kin Fong Lei Alvin Chao-Yu Chen |
author_facet | Chih-Hao Chiu Rei Higashikawa Wen-Ling Yeh Kin Fong Lei Alvin Chao-Yu Chen |
author_sort | Chih-Hao Chiu |
collection | DOAJ |
description | The platelet-rich plasma (PRP) has become an attractive topic for soft tissue healing therapy recently. While some clinical reports revealed the effective treatments for knee osteoarthritis, lateral epicondylitis, and rotator cuff tears, other case studies showed that there was no statistically significant healing improvement. The efficacy of the PRP therapy is still unclear clinically. Thus, a significant amount of basic studies should be conducted to optimize the preparation procedure and the platelet concentration of the PRP. In this work, a 3-chamber co-culture device was developed for the PRP study in order to reduce the usage of primary cells and to avoid the PRP gelation effect. The device was a culture, well partitioning into 3 sub-chambers. Tenocytes and PRP could be respectively loaded into the sub-chambers and co-cultured under the interlinked medium. The results showed that a higher platelet number in the PRP could diffuse higher concentration of the growth factors in the medium and induce higher tenocyte proliferation. The 3-chamber co-culture device provides a simple and practical tool for the PRP study. It is potentially applied for optimizing the preparation procedure and platelet concentration of the PRP therapy. |
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issn | 2072-666X |
language | English |
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spelling | doaj.art-16142d1ec3144a8d9180498fd7ed8d4c2022-12-22T03:18:26ZengMDPI AGMicromachines2072-666X2018-09-019944610.3390/mi9090446mi9090446Investigation of Growth Factor and Tenocyte Proliferation Induced by Platelet Rich Plasma (PRP) in a 3-Chamber Co-Culture DeviceChih-Hao Chiu0Rei Higashikawa1Wen-Ling Yeh2Kin Fong Lei3Alvin Chao-Yu Chen4Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Taoyuan 333, TaiwanDepartment of Orthopedic Surgery, Chang Gung Memorial Hospital, Taoyuan 333, TaiwanBone and Joint Research Center, Chang Gung Memorial Hospital, Linkou 333, TaiwanGraduate Institute of Biomedical Engineering, Chang Gung University, Taoyuan 333, TaiwanBone and Joint Research Center, Chang Gung Memorial Hospital, Linkou 333, TaiwanThe platelet-rich plasma (PRP) has become an attractive topic for soft tissue healing therapy recently. While some clinical reports revealed the effective treatments for knee osteoarthritis, lateral epicondylitis, and rotator cuff tears, other case studies showed that there was no statistically significant healing improvement. The efficacy of the PRP therapy is still unclear clinically. Thus, a significant amount of basic studies should be conducted to optimize the preparation procedure and the platelet concentration of the PRP. In this work, a 3-chamber co-culture device was developed for the PRP study in order to reduce the usage of primary cells and to avoid the PRP gelation effect. The device was a culture, well partitioning into 3 sub-chambers. Tenocytes and PRP could be respectively loaded into the sub-chambers and co-cultured under the interlinked medium. The results showed that a higher platelet number in the PRP could diffuse higher concentration of the growth factors in the medium and induce higher tenocyte proliferation. The 3-chamber co-culture device provides a simple and practical tool for the PRP study. It is potentially applied for optimizing the preparation procedure and platelet concentration of the PRP therapy.http://www.mdpi.com/2072-666X/9/9/446platelet-rich plasmaPRPtenocytesco-culturemicrodevices |
spellingShingle | Chih-Hao Chiu Rei Higashikawa Wen-Ling Yeh Kin Fong Lei Alvin Chao-Yu Chen Investigation of Growth Factor and Tenocyte Proliferation Induced by Platelet Rich Plasma (PRP) in a 3-Chamber Co-Culture Device Micromachines platelet-rich plasma PRP tenocytes co-culture microdevices |
title | Investigation of Growth Factor and Tenocyte Proliferation Induced by Platelet Rich Plasma (PRP) in a 3-Chamber Co-Culture Device |
title_full | Investigation of Growth Factor and Tenocyte Proliferation Induced by Platelet Rich Plasma (PRP) in a 3-Chamber Co-Culture Device |
title_fullStr | Investigation of Growth Factor and Tenocyte Proliferation Induced by Platelet Rich Plasma (PRP) in a 3-Chamber Co-Culture Device |
title_full_unstemmed | Investigation of Growth Factor and Tenocyte Proliferation Induced by Platelet Rich Plasma (PRP) in a 3-Chamber Co-Culture Device |
title_short | Investigation of Growth Factor and Tenocyte Proliferation Induced by Platelet Rich Plasma (PRP) in a 3-Chamber Co-Culture Device |
title_sort | investigation of growth factor and tenocyte proliferation induced by platelet rich plasma prp in a 3 chamber co culture device |
topic | platelet-rich plasma PRP tenocytes co-culture microdevices |
url | http://www.mdpi.com/2072-666X/9/9/446 |
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