Reference Interval of Serum Cystatin C in Dravidian Population Subset of South Asian Ethnic Groups: An Observational Cross-sectional Study
Introduction: Serum Cystatin C (Cys C) facilitates to detect mild renal dysfunction and overall risk of death in older patients with chronic renal diseases. The requirement for creating a particular reference interval is mandatory for such renal diseases in South Asian population since the value of...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2022-10-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/16965/58894_F[SH]_PF1(SC_SS)_PFA(SS)_PB(SC_SS)_PN(SS).pdf |
| Summary: | Introduction: Serum Cystatin C (Cys C) facilitates to detect mild renal dysfunction and overall risk of death in older patients with chronic renal diseases. The requirement for creating a particular reference interval is mandatory for such renal diseases in South Asian population since the value of serum creatinine varies largely with age, gender and population/ethnicity and data of same is short for South Asian population.
Aim: To determine the reference interval of serum Cystatin C amongst the Dravidian population subset of South Asian ethnic groups.
Materials and Methods: This observational cross-sectional study was conducted in Department of Biochemistry and Transfusion Medicine at Government Medical College, Thrissur, Kerala, India, from July 2020 to January 2021 among on 235 healthy adults (20- 60 years). A 4 mL of blood was drawn by aseptic precautions and serum was separated within three hours of blood collection. Serum Cys C was assayed by latex enhanced immunoturbidimetric cystatin C assay using a calibrator traceable to international standard European Specific Protein Reference Material (ERM
DA-471) developed by the International Federation of Clinical Chemistry (IFCC) and Laboratory Medicine. Serum Creatinine (sCr) by Jaffe method in a fully automatic clinical chemistry analyser was assessed. Categorical variables are presented using frequency (percentage) while continuous variables are summarised with an interpercentile range. The Kolmogorov-Smirnov test was used to assess the normality of the distribution of continuous variables.
Results: Total of 235 subjects (20-60 years of age with 17 males
and 218 females) were included in the study. The non parametric reference intervals for Cys C were found to be from 0.39 to 0.79 mg% and that of sCr ranged from 0.79 to 1.2 mg%. The relationship between Cystatin C with age showed an increase in Cystatin C levels as age advances; Spearman’s rank correlation coefficient (rho=0.197). The weak correlation between Cystatin C and sCr was also observed (rho=0.37; p-value <0.0001).
Conclusion: The level of serum cystatin C (0.39 to 0.79 mg%) can be used as a diagnostic concentration reference interval of the protein that helps to recognise, standardise and establish it as a potential biomarker to detect renal disorders in South Asian ethnic population. |
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| ISSN: | 2249-782X 0973-709X |