Isoorientin ameliorates lipid accumulation by regulating fat browning in palmitate-exposed 3T3-L1 adipocytes
Stimulation of fat browning using natural bioactive products is regarded as one of the promising approaches to treat obesity and insulin resistance. Here, we investigated the physiological effects of isoorientin on glucose uptake and lipid accumulation in insulin resistant 3T3-L1 adipocytes. To achi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Metabolism Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589936820300177 |
| _version_ | 1818870768217358336 |
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| author | Sithandiwe E. Mazibuko-Mbeje Khanyisani Ziqubu Phiwayinkosi V. Dludla Luca Tiano Sonia Silvestri Patrick Orlando Thembeka A. Nyawo Johan Louw Abidemi P. Kappo Christo J.F. Muller |
| author_facet | Sithandiwe E. Mazibuko-Mbeje Khanyisani Ziqubu Phiwayinkosi V. Dludla Luca Tiano Sonia Silvestri Patrick Orlando Thembeka A. Nyawo Johan Louw Abidemi P. Kappo Christo J.F. Muller |
| author_sort | Sithandiwe E. Mazibuko-Mbeje |
| collection | DOAJ |
| description | Stimulation of fat browning using natural bioactive products is regarded as one of the promising approaches to treat obesity and insulin resistance. Here, we investigated the physiological effects of isoorientin on glucose uptake and lipid accumulation in insulin resistant 3T3-L1 adipocytes. To achieve this, 3T3-L1 adipocytes were exposed to 0.75 mM palmitate for 24 h, to induce insulin resistance, before treatment with 10 μM isoorientin or the comparative controls such as CL-316,243 (10 μM), pioglitazone (10 μM) and compound C (1 μM) for 4 h. Relevant bioassays and Western blot analysis were conducted on these insulin resistant cells. Our results showed that palmitate exposure could induce insulin resistance and mitochondrial dysfunction as measured by reduction in glucose uptake and impaired mitochondrial bioenergetics parameters. However, treatment with isoorientin reversed these effects by improving glucose uptake, blocking lipid accumulation, and modulating the process of mitochondrial respiration. Mechanistically, isoorientin could mediate lipid metabolism by activating 5′ AMP-activated protein kinase (AMPK), while also effectively modulating the expression of genes involved in fat browning such as peroxisome proliferator-activated receptor gamma (PPAR)γ/α and uncoupling protein 1 (UCP1). In conclusion, isoorientin impacts insulin resistance in vitro by improving glucose uptake and mitochondrial function, consistent to modulating the expression of genes involved in energy metabolism and fat browning. |
| first_indexed | 2024-12-19T12:12:16Z |
| format | Article |
| id | doaj.art-16153e8cdfdf4f459f9bf3abce29c3e7 |
| institution | Directory Open Access Journal |
| issn | 2589-9368 |
| language | English |
| last_indexed | 2024-12-19T12:12:16Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolism Open |
| spelling | doaj.art-16153e8cdfdf4f459f9bf3abce29c3e72022-12-21T20:22:09ZengElsevierMetabolism Open2589-93682020-06-016100037Isoorientin ameliorates lipid accumulation by regulating fat browning in palmitate-exposed 3T3-L1 adipocytesSithandiwe E. Mazibuko-Mbeje0Khanyisani Ziqubu1Phiwayinkosi V. Dludla2Luca Tiano3Sonia Silvestri4Patrick Orlando5Thembeka A. Nyawo6Johan Louw7Abidemi P. Kappo8Christo J.F. Muller9Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, 7505, South Africa; Division of Medical Physiology, Faculty of Health Sciences, Stellenbosch University, Tygerberg, 7505, South Africa; Department of Biochemistry, Faculty of Natural and Agricultural Sciences, North West University, Mafikeng Campus, Mmabatho, 2735, South Africa; Corresponding author. Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, 7505, South Africa.Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, 7505, South Africa; Department of Biochemistry and Microbiology, University of Zululand, KwaDlangezwa, 3886, South AfricaBiomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, 7505, South Africa; Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona 60131, ItalyDepartment of Life and Environmental Sciences, Polytechnic University of Marche, Ancona 60131, ItalyDepartment of Life and Environmental Sciences, Polytechnic University of Marche, Ancona 60131, ItalyDepartment of Life and Environmental Sciences, Polytechnic University of Marche, Ancona 60131, ItalyBiomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, 7505, South Africa; Division of Medical Physiology, Faculty of Health Sciences, Stellenbosch University, Tygerberg, 7505, South AfricaBiomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, 7505, South Africa; Department of Biochemistry and Microbiology, University of Zululand, KwaDlangezwa, 3886, South AfricaDepartment of Biochemistry and Microbiology, University of Zululand, KwaDlangezwa, 3886, South Africa; Department of Biochemistry, Faculty of Science, University of Johannesburg, Kingsway Campus, Auckland Park, 2006, South AfricaBiomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, 7505, South Africa; Division of Medical Physiology, Faculty of Health Sciences, Stellenbosch University, Tygerberg, 7505, South Africa; Department of Biochemistry and Microbiology, University of Zululand, KwaDlangezwa, 3886, South AfricaStimulation of fat browning using natural bioactive products is regarded as one of the promising approaches to treat obesity and insulin resistance. Here, we investigated the physiological effects of isoorientin on glucose uptake and lipid accumulation in insulin resistant 3T3-L1 adipocytes. To achieve this, 3T3-L1 adipocytes were exposed to 0.75 mM palmitate for 24 h, to induce insulin resistance, before treatment with 10 μM isoorientin or the comparative controls such as CL-316,243 (10 μM), pioglitazone (10 μM) and compound C (1 μM) for 4 h. Relevant bioassays and Western blot analysis were conducted on these insulin resistant cells. Our results showed that palmitate exposure could induce insulin resistance and mitochondrial dysfunction as measured by reduction in glucose uptake and impaired mitochondrial bioenergetics parameters. However, treatment with isoorientin reversed these effects by improving glucose uptake, blocking lipid accumulation, and modulating the process of mitochondrial respiration. Mechanistically, isoorientin could mediate lipid metabolism by activating 5′ AMP-activated protein kinase (AMPK), while also effectively modulating the expression of genes involved in fat browning such as peroxisome proliferator-activated receptor gamma (PPAR)γ/α and uncoupling protein 1 (UCP1). In conclusion, isoorientin impacts insulin resistance in vitro by improving glucose uptake and mitochondrial function, consistent to modulating the expression of genes involved in energy metabolism and fat browning.http://www.sciencedirect.com/science/article/pii/S2589936820300177IsoorientinInsulin resistanceLipid accumulationFat browning3T3-L1 adipocytes |
| spellingShingle | Sithandiwe E. Mazibuko-Mbeje Khanyisani Ziqubu Phiwayinkosi V. Dludla Luca Tiano Sonia Silvestri Patrick Orlando Thembeka A. Nyawo Johan Louw Abidemi P. Kappo Christo J.F. Muller Isoorientin ameliorates lipid accumulation by regulating fat browning in palmitate-exposed 3T3-L1 adipocytes Metabolism Open Isoorientin Insulin resistance Lipid accumulation Fat browning 3T3-L1 adipocytes |
| title | Isoorientin ameliorates lipid accumulation by regulating fat browning in palmitate-exposed 3T3-L1 adipocytes |
| title_full | Isoorientin ameliorates lipid accumulation by regulating fat browning in palmitate-exposed 3T3-L1 adipocytes |
| title_fullStr | Isoorientin ameliorates lipid accumulation by regulating fat browning in palmitate-exposed 3T3-L1 adipocytes |
| title_full_unstemmed | Isoorientin ameliorates lipid accumulation by regulating fat browning in palmitate-exposed 3T3-L1 adipocytes |
| title_short | Isoorientin ameliorates lipid accumulation by regulating fat browning in palmitate-exposed 3T3-L1 adipocytes |
| title_sort | isoorientin ameliorates lipid accumulation by regulating fat browning in palmitate exposed 3t3 l1 adipocytes |
| topic | Isoorientin Insulin resistance Lipid accumulation Fat browning 3T3-L1 adipocytes |
| url | http://www.sciencedirect.com/science/article/pii/S2589936820300177 |
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