B Lymphocyte Subsets in Children With Steroid-Sensitive Nephrotic Syndrome: A Longitudinal Study
Background: B-cell subsets may be involved in the pathogenesis of childhood steroid-sensitive nephrotic syndrome (SSNS). Horizontal control studies have shown that homeostasis of B-cell subsets changes at different stages of the SSNS. However, there is a lack of longitudinal studies that have invest...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-10-01
|
| Series: | Frontiers in Pediatrics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fped.2021.736341/full |
| _version_ | 1818394416348397568 |
|---|---|
| author | Chen Ling Chen Ling Zhi Chen Zhi Chen Xiaolin Wang Xiaolin Wang Lin Hua Jingang Gui Jingang Gui Xiaorong Liu Xiaorong Liu |
| author_facet | Chen Ling Chen Ling Zhi Chen Zhi Chen Xiaolin Wang Xiaolin Wang Lin Hua Jingang Gui Jingang Gui Xiaorong Liu Xiaorong Liu |
| author_sort | Chen Ling |
| collection | DOAJ |
| description | Background: B-cell subsets may be involved in the pathogenesis of childhood steroid-sensitive nephrotic syndrome (SSNS). Horizontal control studies have shown that homeostasis of B-cell subsets changes at different stages of the SSNS. However, there is a lack of longitudinal studies that have investigated dynamic changes in B cell subpopulations.Methods: Blood samples were collected at the following time points from 15 children with SSNS treated at our hospital: before administration of steroid and after 3 days, 1 week, and 3, 6, 9, and 12 months. The proportions of circulating total B cells (CD19+), transitional B cells (CD19+CD24highCD38high), mature B cells (CD19+CD24lowCD38intermediate), and memory B cells (CD19+CD24highCD38−) were monitored by flow cytometry.Results: The proportion of CD19+ B cells before steroid administration was significantly higher than that observed at any other time point or in the healthy control (HC) group (p < 0.001). However, this proportion was significantly lower than that in the HC group at 12 months (p = 0.031). Transitional B cells before (%BL 9.5 ± 4.4) and 3 days after steroid administration (%BL 10.6 ± 5.1) were significantly higher than at any other time point or in the HC group (p < 0.001). Although these cells declined after the 3rd day the percentage was still significantly lower than that of the HC group at 12 months (p = 0.029). Memory B cells increased gradually after steroid administration and decreased to the normal range after 9 months.Conclusions: B cell subpopulations show dynamic changes in children with SSNS, suggesting that they are involved in the pathogenesis of the disorder. Further studies are required to determine whether this change can guide individualized treatment. |
| first_indexed | 2024-12-14T06:00:51Z |
| format | Article |
| id | doaj.art-1615b8dcc3364e3f8ee322afede39add |
| institution | Directory Open Access Journal |
| issn | 2296-2360 |
| language | English |
| last_indexed | 2024-12-14T06:00:51Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pediatrics |
| spelling | doaj.art-1615b8dcc3364e3f8ee322afede39add2022-12-21T23:14:26ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602021-10-01910.3389/fped.2021.736341736341B Lymphocyte Subsets in Children With Steroid-Sensitive Nephrotic Syndrome: A Longitudinal StudyChen Ling0Chen Ling1Zhi Chen2Zhi Chen3Xiaolin Wang4Xiaolin Wang5Lin Hua6Jingang Gui7Jingang Gui8Xiaorong Liu9Xiaorong Liu10Department of Nephrology, Beijing Children's Hospital, Capital Medical University, Beijing, ChinaNational Center for Children's Health, Beijing, ChinaDepartment of Nephrology, Beijing Children's Hospital, Capital Medical University, Beijing, ChinaNational Center for Children's Health, Beijing, ChinaKey Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital, Capital Medical University, Beijing, ChinaLaboratory of Immunology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, ChinaSchool of Biomedical Engineering, Capital Medical University, Beijing, ChinaKey Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital, Capital Medical University, Beijing, ChinaLaboratory of Immunology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, ChinaDepartment of Nephrology, Beijing Children's Hospital, Capital Medical University, Beijing, ChinaNational Center for Children's Health, Beijing, ChinaBackground: B-cell subsets may be involved in the pathogenesis of childhood steroid-sensitive nephrotic syndrome (SSNS). Horizontal control studies have shown that homeostasis of B-cell subsets changes at different stages of the SSNS. However, there is a lack of longitudinal studies that have investigated dynamic changes in B cell subpopulations.Methods: Blood samples were collected at the following time points from 15 children with SSNS treated at our hospital: before administration of steroid and after 3 days, 1 week, and 3, 6, 9, and 12 months. The proportions of circulating total B cells (CD19+), transitional B cells (CD19+CD24highCD38high), mature B cells (CD19+CD24lowCD38intermediate), and memory B cells (CD19+CD24highCD38−) were monitored by flow cytometry.Results: The proportion of CD19+ B cells before steroid administration was significantly higher than that observed at any other time point or in the healthy control (HC) group (p < 0.001). However, this proportion was significantly lower than that in the HC group at 12 months (p = 0.031). Transitional B cells before (%BL 9.5 ± 4.4) and 3 days after steroid administration (%BL 10.6 ± 5.1) were significantly higher than at any other time point or in the HC group (p < 0.001). Although these cells declined after the 3rd day the percentage was still significantly lower than that of the HC group at 12 months (p = 0.029). Memory B cells increased gradually after steroid administration and decreased to the normal range after 9 months.Conclusions: B cell subpopulations show dynamic changes in children with SSNS, suggesting that they are involved in the pathogenesis of the disorder. Further studies are required to determine whether this change can guide individualized treatment.https://www.frontiersin.org/articles/10.3389/fped.2021.736341/fullsteroid-sensitive nephrotic syndromeB cellschildrenflow cytometrya longitudinal study |
| spellingShingle | Chen Ling Chen Ling Zhi Chen Zhi Chen Xiaolin Wang Xiaolin Wang Lin Hua Jingang Gui Jingang Gui Xiaorong Liu Xiaorong Liu B Lymphocyte Subsets in Children With Steroid-Sensitive Nephrotic Syndrome: A Longitudinal Study Frontiers in Pediatrics steroid-sensitive nephrotic syndrome B cells children flow cytometry a longitudinal study |
| title | B Lymphocyte Subsets in Children With Steroid-Sensitive Nephrotic Syndrome: A Longitudinal Study |
| title_full | B Lymphocyte Subsets in Children With Steroid-Sensitive Nephrotic Syndrome: A Longitudinal Study |
| title_fullStr | B Lymphocyte Subsets in Children With Steroid-Sensitive Nephrotic Syndrome: A Longitudinal Study |
| title_full_unstemmed | B Lymphocyte Subsets in Children With Steroid-Sensitive Nephrotic Syndrome: A Longitudinal Study |
| title_short | B Lymphocyte Subsets in Children With Steroid-Sensitive Nephrotic Syndrome: A Longitudinal Study |
| title_sort | b lymphocyte subsets in children with steroid sensitive nephrotic syndrome a longitudinal study |
| topic | steroid-sensitive nephrotic syndrome B cells children flow cytometry a longitudinal study |
| url | https://www.frontiersin.org/articles/10.3389/fped.2021.736341/full |
| work_keys_str_mv | AT chenling blymphocytesubsetsinchildrenwithsteroidsensitivenephroticsyndromealongitudinalstudy AT chenling blymphocytesubsetsinchildrenwithsteroidsensitivenephroticsyndromealongitudinalstudy AT zhichen blymphocytesubsetsinchildrenwithsteroidsensitivenephroticsyndromealongitudinalstudy AT zhichen blymphocytesubsetsinchildrenwithsteroidsensitivenephroticsyndromealongitudinalstudy AT xiaolinwang blymphocytesubsetsinchildrenwithsteroidsensitivenephroticsyndromealongitudinalstudy AT xiaolinwang blymphocytesubsetsinchildrenwithsteroidsensitivenephroticsyndromealongitudinalstudy AT linhua blymphocytesubsetsinchildrenwithsteroidsensitivenephroticsyndromealongitudinalstudy AT jinganggui blymphocytesubsetsinchildrenwithsteroidsensitivenephroticsyndromealongitudinalstudy AT jinganggui blymphocytesubsetsinchildrenwithsteroidsensitivenephroticsyndromealongitudinalstudy AT xiaorongliu blymphocytesubsetsinchildrenwithsteroidsensitivenephroticsyndromealongitudinalstudy AT xiaorongliu blymphocytesubsetsinchildrenwithsteroidsensitivenephroticsyndromealongitudinalstudy |