Elastography of multicellular aggregates submitted to osmo-mechanical stress
Tumors are subjected to mechanical stress generated by their own growth in a confined environment, and by their surrounding tissues. Recent works have focused on the study of the growth of spherical aggregates of cells, spheroids, under controlled confinement or stress. In this study we demonstrate...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2015-01-01
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| Series: | New Journal of Physics |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/1367-2630/17/7/073035 |
| _version_ | 1797751144788787200 |
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| author | Charles-Edouard Leroux Joelle Palmier Albert Claude Boccara Giovanni Cappello Sylvain Monnier |
| author_facet | Charles-Edouard Leroux Joelle Palmier Albert Claude Boccara Giovanni Cappello Sylvain Monnier |
| author_sort | Charles-Edouard Leroux |
| collection | DOAJ |
| description | Tumors are subjected to mechanical stress generated by their own growth in a confined environment, and by their surrounding tissues. Recent works have focused on the study of the growth of spherical aggregates of cells, spheroids, under controlled confinement or stress. In this study we demonstrate the measurement of spatially and temporally resolved deformation maps inside spheroids while applying an osmo-mechanical stress. We use full field optical coherence tomography, a high resolution imaging technique well suited for real-time measurements of deformation in living tissues under stress. Using the spherical symmetry of the experiment, we compare our data to a mechanical modeling of the spheroid as a continuous medium. We estimate the viscoelastic parameters of spheroids and discuss the apparent tissue anisotropy after the osmo-mechanical stress. |
| first_indexed | 2024-03-12T16:43:11Z |
| format | Article |
| id | doaj.art-1616cf975f8940288335e5a879157ffb |
| institution | Directory Open Access Journal |
| issn | 1367-2630 |
| language | English |
| last_indexed | 2024-03-12T16:43:11Z |
| publishDate | 2015-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | New Journal of Physics |
| spelling | doaj.art-1616cf975f8940288335e5a879157ffb2023-08-08T14:20:40ZengIOP PublishingNew Journal of Physics1367-26302015-01-0117707303510.1088/1367-2630/17/7/073035Elastography of multicellular aggregates submitted to osmo-mechanical stressCharles-Edouard Leroux0Joelle Palmier1Albert Claude Boccara2Giovanni Cappello3Sylvain Monnier4ESPCI ParisTech, PSL Research University , CNRS, Institut Langevin, 1 rue Jussieu, F-75005 Paris, FranceESPCI ParisTech, PSL Research University , CNRS, Institut Langevin, 1 rue Jussieu, F-75005 Paris, FranceESPCI ParisTech, PSL Research University , CNRS, Institut Langevin, 1 rue Jussieu, F-75005 Paris, FranceUniv. Grenoble Alpes , LIPHY, F-38000 Grenoble, FrancePhysicochimie Curie (Institut Curie/CNRS-UMR168/UPMC), Institut Curie, Centre de Recherche , 26 rue dUlm, F-75248 Paris Cedex 05, FranceTumors are subjected to mechanical stress generated by their own growth in a confined environment, and by their surrounding tissues. Recent works have focused on the study of the growth of spherical aggregates of cells, spheroids, under controlled confinement or stress. In this study we demonstrate the measurement of spatially and temporally resolved deformation maps inside spheroids while applying an osmo-mechanical stress. We use full field optical coherence tomography, a high resolution imaging technique well suited for real-time measurements of deformation in living tissues under stress. Using the spherical symmetry of the experiment, we compare our data to a mechanical modeling of the spheroid as a continuous medium. We estimate the viscoelastic parameters of spheroids and discuss the apparent tissue anisotropy after the osmo-mechanical stress.https://doi.org/10.1088/1367-2630/17/7/073035mechanical properties of tissuesoptical coherence tomographycell dynamics00.0020.0042.10 |
| spellingShingle | Charles-Edouard Leroux Joelle Palmier Albert Claude Boccara Giovanni Cappello Sylvain Monnier Elastography of multicellular aggregates submitted to osmo-mechanical stress New Journal of Physics mechanical properties of tissues optical coherence tomography cell dynamics 00.00 20.00 42.10 |
| title | Elastography of multicellular aggregates submitted to osmo-mechanical stress |
| title_full | Elastography of multicellular aggregates submitted to osmo-mechanical stress |
| title_fullStr | Elastography of multicellular aggregates submitted to osmo-mechanical stress |
| title_full_unstemmed | Elastography of multicellular aggregates submitted to osmo-mechanical stress |
| title_short | Elastography of multicellular aggregates submitted to osmo-mechanical stress |
| title_sort | elastography of multicellular aggregates submitted to osmo mechanical stress |
| topic | mechanical properties of tissues optical coherence tomography cell dynamics 00.00 20.00 42.10 |
| url | https://doi.org/10.1088/1367-2630/17/7/073035 |
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