The Antibacterial Effect of Polypropylenimine-G2 Dendrimer on Escherichia coli, Enterobacter cloacae , Bacillus subtilis, and Staphylococcus aureus
Backgrounds and Objectives:Dendrimers are a new class of synthetic macromolecules, which have many applications in medical sciences.This study was carried out with the purpose ofinvestigating the antibacterial effect of polypropylenimine-G2 (PPI-G2) dendrimer on some bacterial species. Methods:In...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Qom University of Medical Sciences
2014-09-01
|
| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| Subjects: | |
| Online Access: | http://journal.muq.ac.ir/article-1-406-en.html |
| _version_ | 1818715583196168192 |
|---|---|
| author | Hassan Izanlo Mohammad Ahmadi Jebelli Gharib Mjidi Mohammad Khazaei Hamid Reza Tashayoe Vahid Vazirirad Hossein Aghababaei Behnam Vakili |
| author_facet | Hassan Izanlo Mohammad Ahmadi Jebelli Gharib Mjidi Mohammad Khazaei Hamid Reza Tashayoe Vahid Vazirirad Hossein Aghababaei Behnam Vakili |
| author_sort | Hassan Izanlo |
| collection | DOAJ |
| description | Backgrounds and Objectives:Dendrimers are a new class of synthetic macromolecules, which have many applications in medical sciences.This study was carried out with the purpose ofinvestigating the antibacterial effect of polypropylenimine-G2 (PPI-G2) dendrimer on some bacterial species.
Methods:In this study, the antibacterial effects of PPI-G2 dendrimer were studied by disk diffusion and microdilution method. PPI-G2 dendrimer in concentrations of 0.5, 5, 50, and 500µg/ml were inoculated onto blank disks and placed in Mueller-Hinton agar media. Zone of inhibition was investigated by bacterial inoculation according to the McFarland standard 0.5. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of PPI-G2 dendrimer in the concentrations of 0.05, 0.5, 5, 50, and 500µg/ml, were determined using microdilution method in nutrient broth media.
Results: In this study, antibacterial activity of dendrimer increased with increasing their concentration in the disk. Zone of inhibition in the concentration of 500μg/ml for E. coli,Enterobacter cloacae, Bacillus subtilis, and Staphylococcus aurouswere 19, 20, 25, and 21mm, respectively. The MIC for Enterobacter cloacae, Bacillus subtilis, and Staphylococcus aurous was 5μg/ml and for E. coli was 500μg/ml. In addition, the MBC for Bacillus subtilis and Staphylococcus aurous was 50μg/ml and for E. coli and Enterobacter cloacae was 500μg/ml.
Conclusion: The findings of this study showed that PPI-G2 dendrimer has antibacterial effects. However, use of the dendrimer for drinking water disinfection requires further and wider studies. |
| first_indexed | 2024-12-17T19:05:40Z |
| format | Article |
| id | doaj.art-161ad994b9a949df9232938d3dfbbb3f |
| institution | Directory Open Access Journal |
| issn | 1735-7799 2008-1375 |
| language | fas |
| last_indexed | 2024-12-17T19:05:40Z |
| publishDate | 2014-09-01 |
| publisher | Qom University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| spelling | doaj.art-161ad994b9a949df9232938d3dfbbb3f2022-12-21T21:35:59ZfasQom University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum1735-77992008-13752014-09-01843443The Antibacterial Effect of Polypropylenimine-G2 Dendrimer on Escherichia coli, Enterobacter cloacae , Bacillus subtilis, and Staphylococcus aureusHassan Izanlo0Mohammad Ahmadi Jebelli1Gharib Mjidi2Mohammad Khazaei3Hamid Reza Tashayoe4Vahid Vazirirad5Hossein Aghababaei6Behnam Vakili7 Qom University of Medical Sciences Kurdistan University of Medical Sciences Qom University of Medical Sciences Tehran University of Medical Sciences Islamic Azad University, Tehran Medical Sciences Branch Islamic Azad University, Qom Branch Islamic Azad University, Sciences & Research Branch National Water & Wastewater Engineering Company Backgrounds and Objectives:Dendrimers are a new class of synthetic macromolecules, which have many applications in medical sciences.This study was carried out with the purpose ofinvestigating the antibacterial effect of polypropylenimine-G2 (PPI-G2) dendrimer on some bacterial species. Methods:In this study, the antibacterial effects of PPI-G2 dendrimer were studied by disk diffusion and microdilution method. PPI-G2 dendrimer in concentrations of 0.5, 5, 50, and 500µg/ml were inoculated onto blank disks and placed in Mueller-Hinton agar media. Zone of inhibition was investigated by bacterial inoculation according to the McFarland standard 0.5. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of PPI-G2 dendrimer in the concentrations of 0.05, 0.5, 5, 50, and 500µg/ml, were determined using microdilution method in nutrient broth media. Results: In this study, antibacterial activity of dendrimer increased with increasing their concentration in the disk. Zone of inhibition in the concentration of 500μg/ml for E. coli,Enterobacter cloacae, Bacillus subtilis, and Staphylococcus aurouswere 19, 20, 25, and 21mm, respectively. The MIC for Enterobacter cloacae, Bacillus subtilis, and Staphylococcus aurous was 5μg/ml and for E. coli was 500μg/ml. In addition, the MBC for Bacillus subtilis and Staphylococcus aurous was 50μg/ml and for E. coli and Enterobacter cloacae was 500μg/ml. Conclusion: The findings of this study showed that PPI-G2 dendrimer has antibacterial effects. However, use of the dendrimer for drinking water disinfection requires further and wider studies.http://journal.muq.ac.ir/article-1-406-en.htmlpoly(propyleneimine)anti-bacterial agentsmicrobial sensitivity testsminimum bactericidal concentrationzone of inhibition. |
| spellingShingle | Hassan Izanlo Mohammad Ahmadi Jebelli Gharib Mjidi Mohammad Khazaei Hamid Reza Tashayoe Vahid Vazirirad Hossein Aghababaei Behnam Vakili The Antibacterial Effect of Polypropylenimine-G2 Dendrimer on Escherichia coli, Enterobacter cloacae , Bacillus subtilis, and Staphylococcus aureus Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum poly(propyleneimine) anti-bacterial agents microbial sensitivity tests minimum bactericidal concentration zone of inhibition. |
| title | The Antibacterial Effect of Polypropylenimine-G2 Dendrimer on Escherichia coli, Enterobacter cloacae , Bacillus subtilis, and Staphylococcus aureus |
| title_full | The Antibacterial Effect of Polypropylenimine-G2 Dendrimer on Escherichia coli, Enterobacter cloacae , Bacillus subtilis, and Staphylococcus aureus |
| title_fullStr | The Antibacterial Effect of Polypropylenimine-G2 Dendrimer on Escherichia coli, Enterobacter cloacae , Bacillus subtilis, and Staphylococcus aureus |
| title_full_unstemmed | The Antibacterial Effect of Polypropylenimine-G2 Dendrimer on Escherichia coli, Enterobacter cloacae , Bacillus subtilis, and Staphylococcus aureus |
| title_short | The Antibacterial Effect of Polypropylenimine-G2 Dendrimer on Escherichia coli, Enterobacter cloacae , Bacillus subtilis, and Staphylococcus aureus |
| title_sort | antibacterial effect of polypropylenimine g2 dendrimer on escherichia coli enterobacter cloacae bacillus subtilis and staphylococcus aureus |
| topic | poly(propyleneimine) anti-bacterial agents microbial sensitivity tests minimum bactericidal concentration zone of inhibition. |
| url | http://journal.muq.ac.ir/article-1-406-en.html |
| work_keys_str_mv | AT hassanizanlo theantibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT mohammadahmadijebelli theantibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT gharibmjidi theantibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT mohammadkhazaei theantibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT hamidrezatashayoe theantibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT vahidvazirirad theantibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT hosseinaghababaei theantibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT behnamvakili theantibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT hassanizanlo antibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT mohammadahmadijebelli antibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT gharibmjidi antibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT mohammadkhazaei antibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT hamidrezatashayoe antibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT vahidvazirirad antibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT hosseinaghababaei antibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus AT behnamvakili antibacterialeffectofpolypropylenimineg2dendrimeronescherichiacolienterobactercloacaebacillussubtilisandstaphylococcusaureus |