Differential conservation and divergence of fertility genes boule and dazl in the rainbow trout.
BACKGROUND: The genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3017096?pdf=render |
| _version_ | 1811264620035309568 |
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| author | Mingyou Li Qian Shen Hongyan Xu Foong Mei Wong Jianzhou Cui Zhendong Li Ni Hong Li Wang Haobin Zhao Bo Ma Yunhan Hong |
| author_facet | Mingyou Li Qian Shen Hongyan Xu Foong Mei Wong Jianzhou Cui Zhendong Li Ni Hong Li Wang Haobin Zhao Bo Ma Yunhan Hong |
| author_sort | Mingyou Li |
| collection | DOAJ |
| description | BACKGROUND: The genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in invertebrates and mammals but a bisexual mitotic and meiotic expression in medaka. How boule and dazl have evolved different expression patterns in diverse organisms has remained unknown. METHODOLOGY AND PRINCIPAL FINDINGS: Here we chose the fish rainbow trout (Oncorhynchus mykiss) as a second lower vertebrate model to investigate the expression of boule and dazl. By molecular cloning and sequence comparison, we identified cDNAs encoding the trout Boule and Dazl proteins, which have a conserved RNA-recognition motif and a maximal similarity to their homologs. By RT-PCR analysis, adult RNA expression of trout boule and dazl is restricted to the gonads of both sexes. By chromogenic and two-color fluorescence in situ hybridization, we revealed bisexual and germline-specific expression of boule and dazl. We found that dazl displays conserved expression throughout gametogenesis and concentrates in the Balbinani's body of early oocytes and the chromatoid body of sperm. Surprisingly, boule exhibits mitotic and meiotic expression in the male but meiosis-specific expression in the female. CONCLUSIONS: Our data underscores differential conservation and divergence of DAZ family genes during vertebrate evolution. We propose a model in which the diversity of boule expression in sex and stage specificity might have resulted from selective loss or gain of its expression in one sex and mitotic germ cells. |
| first_indexed | 2024-04-12T20:07:36Z |
| format | Article |
| id | doaj.art-161eb46656c24b5a943ac6507276f074 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T20:07:36Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-161eb46656c24b5a943ac6507276f0742022-12-22T03:18:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0161e1591010.1371/journal.pone.0015910Differential conservation and divergence of fertility genes boule and dazl in the rainbow trout.Mingyou LiQian ShenHongyan XuFoong Mei WongJianzhou CuiZhendong LiNi HongLi WangHaobin ZhaoBo MaYunhan HongBACKGROUND: The genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in invertebrates and mammals but a bisexual mitotic and meiotic expression in medaka. How boule and dazl have evolved different expression patterns in diverse organisms has remained unknown. METHODOLOGY AND PRINCIPAL FINDINGS: Here we chose the fish rainbow trout (Oncorhynchus mykiss) as a second lower vertebrate model to investigate the expression of boule and dazl. By molecular cloning and sequence comparison, we identified cDNAs encoding the trout Boule and Dazl proteins, which have a conserved RNA-recognition motif and a maximal similarity to their homologs. By RT-PCR analysis, adult RNA expression of trout boule and dazl is restricted to the gonads of both sexes. By chromogenic and two-color fluorescence in situ hybridization, we revealed bisexual and germline-specific expression of boule and dazl. We found that dazl displays conserved expression throughout gametogenesis and concentrates in the Balbinani's body of early oocytes and the chromatoid body of sperm. Surprisingly, boule exhibits mitotic and meiotic expression in the male but meiosis-specific expression in the female. CONCLUSIONS: Our data underscores differential conservation and divergence of DAZ family genes during vertebrate evolution. We propose a model in which the diversity of boule expression in sex and stage specificity might have resulted from selective loss or gain of its expression in one sex and mitotic germ cells.http://europepmc.org/articles/PMC3017096?pdf=render |
| spellingShingle | Mingyou Li Qian Shen Hongyan Xu Foong Mei Wong Jianzhou Cui Zhendong Li Ni Hong Li Wang Haobin Zhao Bo Ma Yunhan Hong Differential conservation and divergence of fertility genes boule and dazl in the rainbow trout. PLoS ONE |
| title | Differential conservation and divergence of fertility genes boule and dazl in the rainbow trout. |
| title_full | Differential conservation and divergence of fertility genes boule and dazl in the rainbow trout. |
| title_fullStr | Differential conservation and divergence of fertility genes boule and dazl in the rainbow trout. |
| title_full_unstemmed | Differential conservation and divergence of fertility genes boule and dazl in the rainbow trout. |
| title_short | Differential conservation and divergence of fertility genes boule and dazl in the rainbow trout. |
| title_sort | differential conservation and divergence of fertility genes boule and dazl in the rainbow trout |
| url | http://europepmc.org/articles/PMC3017096?pdf=render |
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